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Immunology Innate Immunity Complement Classical Pathway

FITC荧光Anti-C3c抗体(ab4212)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (7)

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Immunocytochemistry/ Immunofluorescence - FITC Anti-C3c antibody (ab4212)

    Key features and details

    • FITC Rabbit polyclonal to C3c
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG

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    概述

    • 产品名称

      FITC荧光Anti-C3c抗体
      参阅全部 C3c 一抗
    • 描述

      FITC荧光兔多克隆抗体to C3c
    • 宿主

      Rabbit
    • 偶联物

      FITC. Ex: 493nm, Em: 528nm
    • 特异性

      This antibody reacts with human C3c complement and with the C3c part of C3 and C3b.
    • 经测试应用

      适用于: ICC/IFmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat, Sheep, Goat, Guinea pig, Cow, Cat, Dog, Pig, Kangaroo, Mink
    • 免疫原

      Full length native protein (purified) corresponding to Human C3c. C3c complement isolated from complement activated human serum.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C.
    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituent: 1% BSA
    • Concentration information loading...
    • 纯度

      IgG fraction
    • 纯化说明

      Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Immunology
      • Innate Immunity
      • Complement
      • Classical Pathway
      • Immunology
      • Innate Immunity
      • Complement
      • Alternative Pathway

    相关产品

    • Isotype control

      • FITC-Rabbit IgG - Isotype Control (ab37406)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4212于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    Use a concentration of 1 µg/ml.
    说明
    ICC/IF
    Use a concentration of 1 µg/ml.

    靶标

    • 功能

      C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
      Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • 组织特异性

      Plasma.
    • 疾病相关

      Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
      Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
      Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
    • 序列相似性

      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • 翻译后修饰

      C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
      Phosphorylation sites are present in the extracelllular medium.
    • 细胞定位

      Secreted.
    • Target information above from: UniProt accession P01024 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 476728 Dog
      • Entrez Gene: 718 Human
      • Entrez Gene: 12266 Mouse
      • Entrez Gene: 397072 Pig
      • Entrez Gene: 24232 Rat
      • Omim: 120700 Human
      • SwissProt: P01024 Human
      • SwissProt: P01027 Mouse
      • SwissProt: P01025 Pig
      • SwissProt: P01026 Rat
      • Unigene: 529053 Human
      • Unigene: 19131 Mouse
      • Unigene: 11378 Rat
      see all
    • 形式

      Cleaved into the following 10 chains: 1) Complement C3 beta chain 2) Complement C3 alpha chain 3) C3a anaphylatoxin 4) Complement C3b alpha' chain 5) Complement C3c alpha' chain fragment 1 6) Complement C3dg fragment 7) Complement C3g fragment 8) Complement C3d fragment 9) Complement C3f fragment 10) Complement C3c alpha' chain fragment 2
    • 别名

      • acylation-stimulating protein cleavage product antibody
      • AHUS5 antibody
      • ARMD9 antibody
      • ASP antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
      • C3 antibody
      • c3 complement antibody
      • C3a anaphylatoxin antibody
      • C3a antibody
      • C3b antibody
      • CO3_HUMAN antibody
      • Complement C3 antibody
      • Complement C3c alpha' chain fragment 2 antibody
      • Complement C3c antibody
      • Complement component 3 antibody
      • Complement component C3 antibody
      • Complement component C3a antibody
      • Complement component C3b antibody
      • Complement factor 3 antibody
      • CPAMD1 antibody
      • Prepro C3 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - FITC Anti-C3c antibody (ab4212)
      Immunocytochemistry/ Immunofluorescence - FITC Anti-C3c antibody (ab4212)

      ICC/IF image of ab4212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4212, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (7)

    发表研究结果有使用 ab4212?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4212 被引用在 7 文献中.

    • Pan T  et al. Circulating Heme Oxygenase-1 and Complement Activation in Transplant-Associated Thrombotic Microangiopathy. Biol Blood Marrow Transplant N/A:N/A (2019). PubMed: 30871975
    • Li Y  et al. Antidepressant-Like Action of Single Facial Injection of Botulinum Neurotoxin A is Associated with Augmented 5-HT Levels and BDNF/ERK/CREB Pathways in Mouse Brain. Neurosci Bull N/A:N/A (2019). PubMed: 30900142
    • Ispasanie E  et al. Spontaneous point mutations in the capsule synthesis locus leading to structural and functional changes of the capsule in serogroup A meningococcal populations. Virulence 9:1138-1149 (2018). PubMed: 30067453
    • Noone DG  et al. Von Willebrand factor regulates complement on endothelial cells. Kidney Int 90:123-34 (2016). PubMed: 27236750
    • Bahia El Idrissi N  et al. Complement activation at the motor end-plates in amyotrophic lateral sclerosis. J Neuroinflammation 13:72 (2016). PubMed: 27056040
    • Zhang Y  et al. Effect of the glycosyltransferases on the capsular polysaccharide synthesis of Streptococcus suis serotype 2. Microbiol Res 185:45-54 (2016). PubMed: 26946377
    • Kim SJ  et al. Intravitreal human complement factor H in a rat model of laser-induced choroidal neovascularisation. Br J Ophthalmol 97:367-70 (2013). Rat . PubMed: 23258212

    客户评价及客户问答

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    1-6 of 6 Abreviews or Q&A

    Flow Cytometry abreview for Anti-C3c antibody (FITC)

    Good
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Sample
    Human Cell (ovary)
    Specification
    ovary
    Preparation
    Cell harvesting/tissue preparation method: trypsinisation
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    live cells
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 05 2007

    Question

    Good Morning,

    I have purchased the following product and I was wondering if you would be able to tell me the concentration of antibody:

    Anti-C3c antibody (FITC) (ab4212)

    Thanking you in advance

    Read More

    Abcam community

    Verified customer

    Asked on Aug 20 2012

    Answer

    Thank you for your enquiry and your interest.

    I can confirm that the Ig concentration for this antibody is 1.4g/l.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Aug 20 2012

    Question

    We are interested in buying the following FITC conjugated antibodies on OEM basis. Could you please let us know the price and other technical details?



    · C1q antibody (FITC)

    · C3c antibody (FITC)

    · Human Kappa Chain antibody [SB81a] (FITC)

    · Kappa light chain antibody (FITC)

    · Lambda Light chain antibody (FITC)

    · Mouse monoclonal [JDC-12] Secondary Antibody to Human lambda - light chain (FITC)

    · Mouse monoclonal [A9604D2] Secondary Antibody to Human IgA2 - Fc (FITC)

    Read More

    Abcam community

    Verified customer

    Asked on Jul 30 2012

    Answer

    Thank you for contacting us.

    My colleague has confirmed that we do not currently OEM any of our products for resale. If you are interested in using our products to develop other assays such as kits, my colleagues at mailto:sales@abcam.com can help you with bulk discount information. Unfortunately, we would not be able to provide a quote for reselling the products directly.

    The products fitting your description are:

    ab4223: C1q antibody (FITC)
    ab4212: C3c antibody (FITC)
    ab79118: Human Kappa Chain antibody [SB81a] (FITC)
    ab4208: Kappa light chain antibody (FITC)
    ab9006: Lambda light chain antibody (FITC)
    ab99809: Mouse monoclonal [JDC-12] Secondary Antibody to Human lambda - light chain (FITC)
    ab99799: Mouse monoclonal [A9604D2] Secondary Antibody to Human IgA2 - Fc (FITC)

    The datasheets with the most current information and pricing are linked below. I hope this helps, please let me know if you need any additional information or assistance.

    Read More

    Abcam Scientific Support

    回复于 Jul 30 2012

    Question

    Product wrongly suggested for IHC - on frozen tissue that has been perfusion fixed. I would like to request a different antibody, ab11887

    Read More

    Abcam community

    Verified customer

    Asked on Feb 08 2012

    Answer

    Thank you for contacting Abcam.


    I do apologize for the error in recommending a suitable product for your experiments. I have placed another order, free of charge. I wish you the best of luck with your experiments!

    Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:

    - Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
    - Return all documents (or copies of) that arrived with the product (if they are available – should be invoice, delivery note and datasheet)
    - Free shipping: Please return via FEDEX PRIORITY OVERNIGHT SHIPPING and use the Fedex account number provided by Abcam
    - Ship to the following address:

    Attn: Returns ORDER REF: (_____)
    Abcam Inc
    One Kendall Square
    Bldg 200, Suite 341
    Cambridge, MA 02139

    There will be no restock fee on this order due to the error being on our end. The most up-to-date information regarding your order, invoice, and credit note is available online in your Abcam account information. If your order was placed by credit card, your credit card will be issued the refund amount. Please be advised that if your order was placed by Purchase Order, all customers have to submit a credit note with their invoice to their Accounts Payable department to be handled.

    We apologize for this inconvenience you may have been caused by this error. If you have any other questions please do not hesitate to contact us.

    Email: mailto:US.orders@Abcam.com
    Phone: 888-77-ABCAM(22226)
    Fax: 866 739 9884
    Customer Service hours of operation: Monday-Friday 8:30 AM-9:00PM EST

    Read More

    Abcam Scientific Support

    回复于 Feb 08 2012

    Question

    BATCH NUMBER 63378 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No staining SAMPLE Rat Kidney Tissue PRIMARY ANTIBODY 100 or 25 fold dilution Reaction was performed at room tempature for 1 hour SECONDARY ANTIBODY Direct observation of primary antibody POSITIVE AND NEGATIVE CONTROLS USED They did not use the possitive control, however they don't know which is best as possitive control. If you possible, please let me know which sample is recomended. They use sample without primary antibody as negative control. ANTIBODY STORAGE CONDITIONS On ice, without freezing FIXATION OF SAMPLE Without fixation ANTIGEN RETRIEVAL No information PERMEABILIZATION STEP No information BLOCKING CONDITIONS Without blocking HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES Customer hope you to give him some advice on protcol or better possitive control. Please let me know.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 28 2005

    Answer

    I can confirm that the recommended positive control must be human kidney and that the tissue should be fixed in acetone. Unfixed tissue may not be suitable for staining. I hope this information helps, please do not hesitate to contact us if you need further advice,

    Read More

    Abcam Scientific Support

    回复于 Mar 02 2005

    Question

    BATCH NUMBER 63378 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No staining SAMPLE Rat Kidney Tissue PRIMARY ANTIBODY 100 or 25 fold dilution Reaction was performed at room tempature for 1 hour SECONDARY ANTIBODY Direct observation of primary antibody POSITIVE AND NEGATIVE CONTROLS USED They did not use the possitive control, however they don't know which is best as possitive control. If you possible, please let me know which sample is recomended. They use sample without primary antibody as negative control. ANTIBODY STORAGE CONDITIONS On ice, without freezing FIXATION OF SAMPLE Without fixation ANTIGEN RETRIEVAL No information PERMEABILIZATION STEP No information BLOCKING CONDITIONS Without blocking HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES Customer hope you to give him some advice on protcol or better possitive control. Please let me know.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 28 2005

    Answer

    I'm sorry to hear you are having a problem with ab4212. I would like to suggest the following modifications to your protocol: -make sure the antibody is protected from the light -fix the frozen tissue sections with ice cold acetone or methanol for 5-10min -make sure you dilute both primary and secondary antibodies in PBST (triton 0.3%v/v in PBS) -incubate the antibody overnight at 4C in the dark and make sure the sections are protected from the light at all times. I have contacted the source of ab4212 to find out the recommended positive control, my apologies for the delay. Please let me know if the above suggestions help and do not hesitate to contact us for further advice,

    Read More

    Abcam Scientific Support

    回复于 Feb 28 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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