AB4212

FITC荧光Anti-C3c抗体

Name: FITC Anti-C3c抗体 (ab4212)| Abcam中文官网
Brand: Abcam Limited
SKU: AB4212
Price: 4052 CNY
Availability: InStock
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FITC Anti-C3c antibody

(1 Review)

(16 Publications)

Rabbit Polyclonal C3 antibody - conjugated to FITC. Suitable for ICC/IF and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human C3.

查看别名

CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

1 Images

Immunocytochemistry/ Immunofluorescence - FITC Anti-C3c antibody (AB4212)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - FITC Anti-C3c antibody (AB4212)

ICC/IF image of ab4212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4212, 1μg/ml) overnight at +4°C. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human

应用

ICC/IF

applications

免疫原

Native Full Length Protein corresponding to Human C3.

P01024

特异性

This antibody reacts with human C3c complement and with the C3c part of C3 and C3b.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Goat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Kangaroo": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mink": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯度

IgG fraction

纯化说明

Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins.

存储溶液

Preservative: 0.05% Sodium azide Constituents: 1% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The C3c fragment is part of the complement system which plays an important role in the innate immune system. C3c also known as complement component 3c forms when C3 undergoes cleavage. The molecular mass of C3c ranges from approximately 135 to 186 kDa depending on the level of cleavage. It presents primarily in plasma and serum being part of the larger C3 molecule which is produced by the liver. Within tissues C3c can also appear in inflammatory sites where complement activation occurs.

Biological function summary

C3c functions as a by-product of complement activation and is associated with immune defense processes. It does not independently participate in the formation of the complement complex but results from the breakdown of C3 into C3b and other fragments. This fragment may play roles in modulating immune responses through its interaction with complement receptors. The presence of C3c marks the location and intensity of complement activation in tissue or circulation indirectly indicating the body's response to pathogens or damaged cells.

Pathways

The C3c fragment serves as an indirect marker within the complement cascade particularly signaling the involvement of the classical and alternative complement pathways. The generation of C3c follows the activation of these pathways highlighting the initial activation of C3 into active fragments such as C3b. Proteins related to these pathways include factors H and I which regulate C3b breakdown ensuring controlled progression of this immune response.

C3c levels offer insights into pathological conditions like systemic lupus erythematosus (SLE) and glomerulonephritis. Elevated levels of C3c in serum may indicate excessive complement activation and consumption often observed in these diseases. The association of C3c with disease is often mediated through its precursor C3 with abnormal C3 activation linked to tissue damage in autoimmune conditions. Proteins like C4 and complement receptor (CR1) may also connect to these disorders as they interact in pathways leading to inflammatory responses characterized by complement activity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed : 10432298, PubMed : 15833747, PubMed : 16333141, PubMed : 19615750, PubMed : 2909530, PubMed : 8376604, PubMed : 9059512).

See full target information C3

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Haematologica : PubMed40820810

2025

KLF4 overexpression protects against complement-mediated endothelial injury in transplant-associated thrombotic microangiopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhui Jiang,Jiaqian Qi,Tingting Pan,Zhenzhen Yao,Siyi Lu,Yifei Han,Depei Wu,Yue Han

Nature communications 16:2666 PubMed40102474

2025

Complement-mediated enhancement of SARS-CoV-2 antibody neutralisation potency in vaccinated individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Jack Mellors,Raman Dhaliwal,Stephanie Longet,Tom Tipton,Eleanor Barnes,Susanna J Dunachie,Paul Klenerman,Julian Hiscox,Miles Carroll

Cell death & disease 15:592 PubMed39147758

2024

Astrocytes and the tumor microenvironment inflammatory state dictate the killing of glioblastoma cells by Smac mimetic compounds.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle Malone,Melanie Dugas,Nathalie Earl,Tommy Alain,Eric C LaCasse,Shawn T Beug

Scientific reports 14:11020 PubMed38745067

2024

The STING inhibitor (ISD-017) reduces glomerulonephritis in 129.B6.Fcgr2b-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Isara Alee,Papasara Chantawichitwong,Asada Leelahavanichkul,Søren R Paludan,Trairak Pisitkun,Prapaporn Pisitkun

Journal of dental sciences 18:1008-1015 PubMed37404619

2023

Clinical, histological and direct immunofluorescence features in oral mucosal patches striae diseases with malignant potential.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Lei Li,Xiao-Meng Ren,Xin Fang,Hai-Yan Luo,Hong Hua

Frontiers in medicine 10:891513 PubMed36860338

2023

The loss of glycocalyx integrity impairs complement factor H binding and contributes to cyclosporine-induced endothelial cell injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chia Wei Teoh,Magdalena Riedl Khursigara,Carolina G Ortiz-Sandoval,Jee Woo Park,Jun Li,Arlette Bohorquez-Hernandez,Valentina Bruno,Emily E Bowen,Spencer A Freeman,Lisa A Robinson,Christoph Licht

British journal of haematology 199:603-615 PubMed35864790

2022

Upregulation of HIF-1α contributes to complement activation in transplantation-associated thrombotic microangiopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqian Qi,Tingting Pan,Tao You,Yaqiong Tang,Tiantian Chu,Jia Chen,Yi Fan,Shuhong Hu,Fei Yang,Changgeng Ruan,Depei Wu,Yue Han

Journal of cellular physiology 236:5012-5021 PubMed33400289

2021

Uromodulin aggravates renal tubulointerstitial injury through activation of the complement pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yu,Fei Pei,Qiaoling Sun,Fei Shen,Xiangdong Yang,Zhao Hu,Maojing Liu

Nature medicine 27:279-288 PubMed33335322

2020

Phase 1/2 trial of SARS-CoV-2 vaccine ChAdOx1 nCoV-19 with a booster dose induces multifunctional antibody responses.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan R Barrett,Sandra Belij-Rammerstorfer,Christina Dold,Katie J Ewer,Pedro M Folegatti,Ciaran Gilbride,Rachel Halkerston,Jennifer Hill,Daniel Jenkin,Lisa Stockdale,Marije K Verheul,Parvinder K Aley,Brian Angus,Duncan Bellamy,Eleanor Berrie,Sagida Bibi,Mustapha Bittaye,Miles W Carroll,Breeze Cavell,Elizabeth A Clutterbuck,Nick Edwards,Amy Flaxman,Michelle Fuskova,Andrew Gorringe,Bassam Hallis,Simon Kerridge,Alison M Lawrie,Aline Linder,Xinxue Liu,Meera Madhavan,Rebecca Makinson,Jack Mellors,Angela Minassian,Maria Moore,Yama Mujadidi,Emma Plested,Ian Poulton,Maheshi N Ramasamy,Hannah Robinson,Christine S Rollier,Rinn Song,Matthew D Snape,Richard Tarrant,Stephen Taylor,Kelly M Thomas,Merryn Voysey,Marion E E Watson,Daniel Wright,Alexander D Douglas,Catherine M Green,Adrian V S Hill,Teresa Lambe,Sarah Gilbert,Andrew J Pollard

Neuroscience bulletin 35:661-672 PubMed30900142

2019

Antidepressant-Like Action of Single Facial Injection of Botulinum Neurotoxin A is Associated with Augmented 5-HT Levels and BDNF/ERK/CREB Pathways in Mouse Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Jing Liu,Xu Liu,Cun-Jin Su,Qi-Lin Zhang,Zhi-Hong Wang,Lei-Fang Cao,Xue-Yan Guo,Ya Huang,Weifeng Luo,Tong Liu

View all publications

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FITC荧光Anti-C3c抗体

FITC Anti-C3c antibody

Immunocytochemistry/ Immunofluorescence - FITC Anti-C3c antibody (AB4212)

关键信息

宿主种属

克隆

亚型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用

免疫原

特异性