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Immunology Innate Immunity Complement Classical Pathway

FITC荧光Anti-C1q抗体(ab4223)

  • Datasheet
Reviews (2)Q&A (3)References (6)

Product price, shipping and contact information

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Immunocytochemistry - FITC Anti-C1q antibody (ab4223)
  • Immunocytochemistry - FITC Anti-C1q antibody (ab4223)

Key features and details

  • FITC Rabbit polyclonal to C1q
  • Suitable for: IHC-P, Flow Cyt, ICC
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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概述

  • 产品名称

    FITC荧光Anti-C1q抗体
    参阅全部 C1q 一抗
  • 描述

    FITC荧光兔多克隆抗体to C1q
  • 宿主

    Rabbit
  • 偶联物

    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用

    适用于: IHC-P, Flow Cyt, ICCmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human C1q. C1q isolated from the normal human serum.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 纯化说明

    Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Processes

相关产品

  • Isotype control

    • FITC-Rabbit IgG - Isotype Control (ab37406)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4223于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 21746929

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 21746929

ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC
Use at an assay dependent concentration.

靶标

  • 功能

    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • 疾病相关

    Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • 序列相似性

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • 翻译后修饰

    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • 细胞定位

    Secreted.
  • Target information above from: UniProt accession P02745 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 712 Human
    • Entrez Gene: 713 Human
    • Entrez Gene: 714 Human
    • Omim: 120550 Human
    • Omim: 120570 Human
    • Omim: 120575 Human
    • SwissProt: P02745 Human
    • SwissProt: P02746 Human
    • SwissProt: P02747 Human
    • Unigene: 632379 Human
    see all
  • 别名

    • C1qa antibody
    • C1QA_HUMAN antibody
    • C1QB antibody
    • C1QC antibody
    • Complement C1q subcomponent subunit A antibody
    • Complement C1q subcomponent subunit B antibody
    • Complement C1q subcomponent subunit C antibody
    • Complement component 1 q subcomponent alpha polypeptide antibody
    • Complement component C1q A chain antibody
    • Complement component C1q B chain antibody
    • Complement component C1q C chain antibody
    see all

图片

  • Immunocytochemistry - FITC Anti-C1q antibody (ab4223)
    Immunocytochemistry - FITC Anti-C1q antibody (ab4223)

    C1q isolated from the NHS. This antibody reacts with the Human C1q complement. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may also be used for other applications.

  • Immunocytochemistry - FITC Anti-C1q antibody (ab4223)
    Immunocytochemistry - FITC Anti-C1q antibody (ab4223)Image from Phuan PW et al., J Biol Chem. 2012 Apr 20;287(17):13829-39. Epub 2012 Mar 5. Fig 7.; doi: 10.1074/jbc.M112.344325; April 20, 2012, The Journal of Biological Chemistry, 287, 13829-13839.
    ab4223, staining C1q in CHO-K1 cells transfected with M1-AQP4 or M23-AQP4 by Immunocytochemistry/ Immunofluorescence.

    Cells were incubated at room temperature with recombinant monoclonal NMO antibody and 120 µg/ml human C1q protein (ab96363). Cells were washed with PBS, fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were blocked with 1% BSA for 30 min and incubated for 60 min with primary antibody against C1q (ab4223) at 1/200 dilution.

实验方案

  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (6)

发表研究结果有使用 ab4223?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab4223 被引用在 6 文献中.

  • Aoyama M  et al. Effects of terminal galactose residues in mannose a1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies. MAbs 11:826-836 (2019). PubMed: 30990348
  • Burbano C  et al. Potential Involvement of Platelet-Derived Microparticles and Microparticles Forming Immune Complexes during Monocyte Activation in Patients with Systemic Lupus Erythematosus. Front Immunol 9:322 (2018). PubMed: 29545790
  • Burbano C  et al. Extracellular vesicles are associated with the systemic inflammation of patients with seropositive rheumatoid arthritis. Sci Rep 8:17917 (2018). PubMed: 30559453
  • Fox S  et al. The role of C1q in recognition of apoptotic epithelial cells and inflammatory cytokine production by phagocytes during Helicobacter pylori infection. J Inflamm (Lond) 12:51 (2015). PubMed: 26357509
  • Phuan PW  et al. Complement-dependent Cytotoxicity in Neuromyelitis Optica Requires Aquaporin-4 Protein Assembly in Orthogonal Arrays. J Biol Chem 287:13829-39 (2012). ICC/IF . PubMed: 22393049
  • Barfod L  et al. Evasion of immunity to Plasmodium falciparum malaria by IgM masking of protective IgG epitopes in infected erythrocyte surface-exposed PfEMP1. Proc Natl Acad Sci U S A 108:12485-90 (2011). Flow Cyt ; Human . PubMed: 21746929

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-C1q antibody (FITC)

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
0.06 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Serum (Serum)
Specification
Serum
Blocking step
Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 15 2014

Immunohistochemistry (Frozen sections) abreview for Anti-C1q antibody (FITC)

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
(agent) for 10 minute(s) · Concentration: 4% · Temperature: 25°C
Sample
Dog Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 01 2014

Question

We are interested in buying the following FITC conjugated antibodies on OEM basis. Could you please let us know the price and other technical details?



· C1q antibody (FITC)

· C3c antibody (FITC)

· Human Kappa Chain antibody [SB81a] (FITC)

· Kappa light chain antibody (FITC)

· Lambda Light chain antibody (FITC)

· Mouse monoclonal [JDC-12] Secondary Antibody to Human lambda - light chain (FITC)

· Mouse monoclonal [A9604D2] Secondary Antibody to Human IgA2 - Fc (FITC)

Read More

Abcam community

Verified customer

Asked on Jul 30 2012

Answer

Thank you for contacting us.

My colleague has confirmed that we do not currently OEM any of our products for resale. If you are interested in using our products to develop other assays such as kits, my colleagues at mailto:sales@abcam.com can help you with bulk discount information. Unfortunately, we would not be able to provide a quote for reselling the products directly.

The products fitting your description are:

ab4223: C1q antibody (FITC)
ab4212: C3c antibody (FITC)
ab79118: Human Kappa Chain antibody [SB81a] (FITC)
ab4208: Kappa light chain antibody (FITC)
ab9006: Lambda light chain antibody (FITC)
ab99809: Mouse monoclonal [JDC-12] Secondary Antibody to Human lambda - light chain (FITC)
ab99799: Mouse monoclonal [A9604D2] Secondary Antibody to Human IgA2 - Fc (FITC)

The datasheets with the most current information and pricing are linked below. I hope this helps, please let me know if you need any additional information or assistance.

Read More

Abcam Scientific Support

回复于 Jul 30 2012

Question

Buen día , como estas?

Te molesto porque tengo una clienta que compró el anticuerpo de Abcam ab4223, y quiere utilizarlo para IF e IHC-P, y quiere saber la dilución aconsejada para comenzar a utilizarlo. Yo traté de bajar las publicaciones que están en la página web pero no son versiones free y no pude acceder.
Espero tu respuesta, millones de gracias como siempre
Saludos,

Read More

Abcam community

Verified customer

Asked on Jul 17 2012

Answer

Gracias por contactarnos de nuevo, ¡no es ninguna molestia!

He revisado los artículos referenciados en la datasheet del producto, y para ICC/IF la dilución recomendada es de 1/200. Te copio el párrafo correspondiente al protocolo usado, por si pudiera resultar de utilidad a la clienta:

C1q and C9neo Immunofluorescence

CHO-K1 cells were plated onto coverslips in 24-well plates and grown at 37 °C/5% CO2 for 18–24 h. For C1q immunofluorescence, cells were washed with Hanks' BSS and incubated at room temperature for 30 min with 400 μl of Hanks' BSS containing 40 μg/ml rAb-58 and 120 μg/ml human C1q protein (Abcam, Cambridge, MA). Cells were washed with PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100. Cells were blocked with 1% BSA for 30 min and incubated for 60 min with 1:200 dilution of FITC-conjugated rabbit polyclonal anti-C1q antibody (Abcam, Cambridge, MA) and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (Santa Cruz Biotechnology). Cells were incubated for 30 min with 5 μg/ml goat anti-rabbit IgG-conjugated Alexa Fluor 555 (http://www.jbc.org/cgi/redirect-inline?ad=Invitrogen) in 1% BSA. For C9neo immunofluorescence, cells were incubated with rAbs as above, together with 2% pooled normal human complement serum. After fixation and permeabilization, cells were incubated for 60 min with 0.1 μg/ml mouse monoclonal anti-C5b-9 (C9neo) antibody and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (both from Santa Cruz Biotechnology). Cells were then incubated for 30 min with 5 μg/ml donkey anti-mouse IgG-conjugated Alexa Fluor 488 and goat anti-rabbit IgG-conjugated Alexa Fluor 555 (http://www.jbc.org/cgi/redirect-inline?ad=Invitrogen) in 1% BSA.

Para Citometría no se menciona en el artículo la dilución empleada, pero nosotros siempre recomendamos usar, para anticuerpos purificados, como es este caso, una concentración de 1ug/ml.

El párrafo al respecto del artículo mencionado se adjunta a continuación:

C1q Deposition on Infected Erythrocytes.

Binding of complement component C1q IEs was measured as described previously (17). In brief, purified late-stage IEs were incubated in PBS (30 min at 4 °C) with or without either IgM or IgG (300 μg/mL), washed three times, incubated (30 min at 4 °C) in Veronal buffer (http://www.oxoid.com) supplemented with either purified human C1q (10 μg/mL; http://www.sigma-aldrich.com) or nonimmune plasma (10%) as the C1q source, washed three times, and labeled (30 min at 4 °C) with FITC-conjugated rabbit anti-human C1q (https://www.abcam.com), anti-human IgG, or anti-human IgM. After two washes in Veronal buffer, the labeled IEs were analyzed by flow cytometry as above.

Espero haber resuelto la duda de la clienta. En caso contrario, por favor no dudes en volverme a contactar, y estaré encantada de poder ayudar.

¡Que pases un día estupendo!

Read More

Abcam Scientific Support

回复于 Jul 17 2012

Question

Customer would like to know the concentration of ab4223.

Read More

Abcam community

Verified customer

Asked on Feb 20 2004

Answer

Approximately 1 mg/ml

Read More

Abcam Scientific Support

回复于 Feb 20 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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