FITC荧光Anti-C1q抗体(ab4223)

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My colleague has confirmed that we do not currently OEM any of our products for resale. If you are interested in using our products to develop other assays such as kits, my colleagues at mailto:sales@abcam.com can help you with bulk discount information. Unfortunately, we would not be able to provide a quote for reselling the products directly.

The products fitting your description are:

ab4223: C1q antibody (FITC)
ab4212: C3c antibody (FITC)
ab79118: Human Kappa Chain antibody [SB81a] (FITC)
ab4208: Kappa light chain antibody (FITC)
ab9006: Lambda light chain antibody (FITC)
ab99809: Mouse monoclonal [JDC-12] Secondary Antibody to Human lambda - light chain (FITC)
ab99799: Mouse monoclonal [A9604D2] Secondary Antibody to Human IgA2 - Fc (FITC)

The datasheets with the most current information and pricing are linked below. I hope this helps, please let me know if you need any additional information or assistance.

Gracias por contactarnos de nuevo, ¡no es ninguna molestia!

He revisado los artículos referenciados en la datasheet del producto, y para ICC/IF la dilución recomendada es de 1/200. Te copio el párrafo correspondiente al protocolo usado, por si pudiera resultar de utilidad a la clienta:

C1q and C9neo Immunofluorescence

CHO-K1 cells were plated onto coverslips in 24-well plates and grown at 37 °C/5% CO2 for 18–24 h. For C1q immunofluorescence, cells were washed with Hanks' BSS and incubated at room temperature for 30 min with 400 μl of Hanks' BSS containing 40 μg/ml rAb-58 and 120 μg/ml human C1q protein (Abcam, Cambridge, MA). Cells were washed with PBS, fixed in 4% paraformaldehyde for 15 min, and permeabilized with 0.1% Triton X-100. Cells were blocked with 1% BSA for 30 min and incubated for 60 min with 1:200 dilution of FITC-conjugated rabbit polyclonal anti-C1q antibody (Abcam, Cambridge, MA) and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (Santa Cruz Biotechnology). Cells were incubated for 30 min with 5 μg/ml goat anti-rabbit IgG-conjugated Alexa Fluor 555 (http://www.jbc.org/cgi/redirect-inline?ad=Invitrogen) in 1% BSA. For C9neo immunofluorescence, cells were incubated with rAbs as above, together with 2% pooled normal human complement serum. After fixation and permeabilization, cells were incubated for 60 min with 0.1 μg/ml mouse monoclonal anti-C5b-9 (C9neo) antibody and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (both from Santa Cruz Biotechnology). Cells were then incubated for 30 min with 5 μg/ml donkey anti-mouse IgG-conjugated Alexa Fluor 488 and goat anti-rabbit IgG-conjugated Alexa Fluor 555 (http://www.jbc.org/cgi/redirect-inline?ad=Invitrogen) in 1% BSA.

Para Citometría no se menciona en el artículo la dilución empleada, pero nosotros siempre recomendamos usar, para anticuerpos purificados, como es este caso, una concentración de 1ug/ml.

El párrafo al respecto del artículo mencionado se adjunta a continuación:

C1q Deposition on Infected Erythrocytes.

Binding of complement component C1q IEs was measured as described previously (17). In brief, purified late-stage IEs were incubated in PBS (30 min at 4 °C) with or without either IgM or IgG (300 μg/mL), washed three times, incubated (30 min at 4 °C) in Veronal buffer (http://www.oxoid.com) supplemented with either purified human C1q (10 μg/mL; http://www.sigma-aldrich.com) or nonimmune plasma (10%) as the C1q source, washed three times, and labeled (30 min at 4 °C) with FITC-conjugated rabbit anti-human C1q (https://www.abcam.com), anti-human IgG, or anti-human IgM. After two washes in Veronal buffer, the labeled IEs were analyzed by flow cytometry as above.

Espero haber resuelto la duda de la clienta. En caso contrario, por favor no dudes en volverme a contactar, y estaré encantada de poder ayudar.

¡Que pases un día estupendo!

Approximately 1 mg/ml