FITC荧光Anti-c-Fos抗体[H15-S] (ab175647)
![Flow Cytometry - FITC Anti-c-Fos antibody [H15-S] (ab175647)](https://www.abcam.cn/ps/products/175/ab175647/Images/ab175647-202347-anti-c-fos-antibody-h15-s-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-c-Fos antibody [H15-S] (ab175647)")
Key features and details
- FITC Rabbit monoclonal [H15-S] to c-Fos
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
概述
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产品名称
FITC荧光Anti-c-Fos抗体[H15-S]
参阅全部 c-Fos 一抗 -
描述
FITC荧光兔单克隆抗体[H15-S] to c-Fos -
宿主
Rabbit -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human c-Fos (C terminal).
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表位
Antibody detects the linear epitope at the C-terminus of human c-FOS -
阳性对照
- Human peripheral blood lymphocytes.
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常规说明
The monospecific product was conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.33% Sodium phosphate
Aqueous buffer -
Concentration information loading... -
纯化说明
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a propriety technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability. -
克隆
单克隆 -
克隆编号
H15-S -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175647于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
(in a 100 µl experimental sample). |
| 说明 |
|---|
|
Flow Cyt
Use 10µl for 106 cells. (in a 100 µl experimental sample). |
靶标
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功能
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. -
序列相似性
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
翻译后修饰
Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2353 Human
- Omim: 164810 Human
- SwissProt: P01100 Human
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别名
- Activator protein 1 antibody
- AP 1 antibody
- C FOS antibody
see all
图片
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Flow Cytometry - FITC Anti-c-Fos antibody [H15-S] (ab175647)
Flow cytometric analysis of permeabilized Human peripheral blood lymphocytes labeling c-Fos with ab175647 at 10μl/1 x 106 cells (blue) compared with an isotype control (black).
数据表及文件
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Datasheet download
文献 (1)
ab175647 被引用在 1 文献中.
- Fast EM et al. External signals regulate continuous transcriptional states in hematopoietic stem cells. Elife 10:N/A (2021). PubMed: 34939923
