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FITC荧光Anti-Biotin抗体(ab6650)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (5)

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Key features and details

  • FITC Goat polyclonal to Biotin
  • Suitable for: Flow Cyt, ICC/IF, Dot blot
  • Reacts with: Species independent
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG

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概述

  • 产品名称

    FITC荧光Anti-Biotin抗体
    参阅全部 Biotin 一抗
  • 描述

    FITC荧光山羊多克隆抗体to生物素
  • 宿主

    Goat
  • 偶联物

    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用

    适用于: Flow Cyt, ICC/IF, Dot blotmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Chemical/ Small Molecule conjugated to KLH

  • 常规说明

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Isotype control

    • FITC Goat IgG - Isotype control (ab37374)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6650于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/500 - 1/2500.
Dot blot
Use at an assay dependent concentration.
说明
Flow Cyt
Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/500 - 1/2500.
Dot blot
Use at an assay dependent concentration.

靶标

  • 相关性

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • 别名

    • Vitamin B4 antibody

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (5)

发表研究结果有使用 ab6650?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6650 被引用在 5 文献中.

  • Siebert A  et al. ALS-linked loss of Cyclin-F function affects HSP90. Life Sci Alliance 5:N/A (2022). PubMed: 36114006
  • Trivedi S  et al. PARG and BRCA1-BARD1 cooperative function regulates DNA repair pathway choice during gametogenesis. Nucleic Acids Res 50:12291-12308 (2022). PubMed: 36478097
  • Dello Stritto MR  et al. DNA topoisomerase 3 is required for efficient germ cell quality control. J Cell Biol 220:N/A (2021). PubMed: 33798260
  • Link J  et al. Transient and Partial Nuclear Lamina Disruption Promotes Chromosome Movement in Early Meiotic Prophase. Dev Cell 45:212-225.e7 (2018). PubMed: 29689196
  • Baratta JL  et al. Cellular organization of normal mouse liver: a histological, quantitative immunocytochemical, and fine structural analysis. Histochem Cell Biol 131:713-26 (2009). IHC-FoFr . PubMed: 19255771

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Hello, Can you tell me how many dyes (fluorescein) are attached per molecule of anti-biotin antibody? Waiting for your reply and thanking you in advance. Cheers.

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Abcam community

Verified customer

Asked on Feb 18 2002

Answer

The molar ratios of dye-to-protein are varied batch to batch. The general acceptance criteria for this product is 2-5:1

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XXXXX XXXXX

Abcam Scientific Support

回复于 Feb 19 2002

Question

Have you used AB6650 (Goat anti-biotin Fluorescein conjugate) for membrane-based detection of Biotinylated nucleic acids on charged nylon membranes (specifically Gene-screen plus)? If so, what membrane blocking reagents have been tested & how did they work? (i.e best, 2nd best etc.)

Read More

Abcam community

Verified customer

Asked on Aug 24 2001

Answer

To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product.

Read More

Abcam Scientific Support

回复于 Aug 29 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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