FITC荧光Anti-Bcl-2抗体[Bcl-2/100] (ab272337)
![Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (ab272337)](https://www.abcam.cn/ps/products/272/ab272337/Images/ab272337-387901-Mouse-monoclonal-to-Bcl2-FITC-flow-cytometry-human-peripheral-blood-lymphocytes.jpg "Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (ab272337)")
Key features and details
- FITC Mouse monoclonal [Bcl-2/100] to Bcl-2
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
概述
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产品名称
FITC荧光Anti-Bcl-2抗体[Bcl-2/100]
参阅全部 Bcl-2 一抗 -
描述
FITC荧光小鼠单克隆抗体[Bcl-2/100] to Bcl-2 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Bcl-2 aa 1-100.
Database link: P10415 -
阳性对照
- Flow Cyt (Intra): Human peripheral whole blood. Bcl-2 positive lymphocytes.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Size exclusion -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
Bcl-2/100 -
同种型
IgG1 -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272337于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. |
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. |
靶标
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功能
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). -
组织特异性
Expressed in a variety of tissues. -
疾病相关
A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. -
序列相似性
Belongs to the Bcl-2 family. -
结构域
BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
The loop between motifs BH4 and BH3 is required for the interaction with NLRP1. -
翻译后修饰
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. -
细胞定位
Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 596 Human
- Omim: 151430 Human
- SwissProt: P10415 Human
- Unigene: 150749 Human
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别名
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
see all
图片
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Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (ab272337)
Flow cytometry intracellular staining pattern of human peripheral whole blood stained usingab272337 at 4 µl reagent / 100 µl of peripheral whole blood.
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![Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (ab272337)](https://www.abcam.cn/ps/products/272/ab272337/Images/ab272337-387902-Mouse-monoclonal-to-Bcl2-FITC-flow-cytometry-bcl2-positive-lymphocytes.jpg)
Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (ab272337)Separation of human Bcl-2 positive lymphocytes (red-filled) from blood debris (black-dashed) in Intracellular Flow Cytometry analysis (intracellular staining) of human peripheral whole blood stained using ab272337 at 4 µl reagent / 100 µl of peripheral whole blood.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272337 尚未被引用在任何文献中。