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Anti-FITC抗体[2A3] (ab10257)

Submit a review Q&A (3)References (2)

Key features and details

  • Mouse monoclonal [2A3] to FITC
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Species independent
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-FITC抗体[2A3]
    参阅全部 FITC 一抗

  • 描述

    小鼠单克隆抗体[2A3] to FITC荧光

  • 宿主

    Mouse

  • 特异性

    ab10257 reacts with both free and conjugated FITC.

  • 经测试应用

    适用于: IHC-P, IHC-Frmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    FITC-BSA conjugate.

  • 常规说明

    Concentration varies from lot to lot and can be provided on request.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Purity tested by electrophoresis.

  • 克隆

    单克隆

  • 克隆编号

    2A3

  • 骨髓瘤

    Sp2/0

  • 同种型

    IgG2a

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab10257于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

靶标

  • 相关性

    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

  • 别名

    • Fluorescein Isothiocyanate antibody

文献 (2)

发表研究结果有使用 ab10257？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab10257 被引用在 2 文献中.

  • Ortenlöf N  et al. Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3. Fluids Barriers CNS 20:59 (2023). PubMed: 37582792
  • Sobolewska B  et al. Human Platelets Take up Anti-VEGF Agents. J Ophthalmol 2021:8811672 (2021). PubMed: 34221496

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Hi, can you tell me how much you have left of the following antibody lot:   Mouse anti-fluorescein antibody: cat # ab10257, lot # GR4859-5  

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Answer

Thank you for contacting us. We have no vials of GR4859-5 but we do have six vials of GR4859-6, which is the same stock as GR4859-5, but aliquotted on a different date. Please do not hesitate to contact us if you need any more advice or information.

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Question

Can this antibody also recognize and bind other fluorescein derivatives, e.g. FAM?

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Answer

Unfortunately ab10257 has not been tested to react with FAM. I am sorry for not being more helpful on this occasion.

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Question

Has this antibody been tested in IHC on frozen or paraffin-embedded sections?

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Answer

Thank you for your enquiry. Ab10257 can be used in IHC in two ways: 1. You can use FITC-labeled antibody against antigen of your interest as first antibody and then apply peroxidase-labeled ab10257 as developing reagent. 2. You can add the excess of native anti-FITC antibody and then FITC-labeled anti-peroxodase antibody mixed with peroxidase (FITC labeled PAP-complex). In both cases paraffin or frozen sections may be used. The detection of antigen of interest will depend on the ability of the first antibody to recognize the antigen and the sensitivity of antigen to procedures necessary for embedding the sample in paraffin.

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