AB8211

FITC荧光Anti-alpha smooth muscle Actin抗体[1A4]

Name: FITC Anti-alpha smooth muscle Actin抗体[1A4] (ab8211)| Abcam中文官网
Brand: Abcam Limited
SKU: AB8211
Rating: 5 (9 reviews)

FITC Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated
了解详情

(9 Reviews)

(71 Publications)

Anti-alpha smooth muscle Actin antibody [1A4] - FITC conjugated (ab8211) is a mouse monoclonal antibody detecting alpha smooth muscle Actin in Western Blot, ICC/IF. Suitable for Human.

- KO validated for confirmed specificity
- Over 70 publications
- Trusted since 2001

查看别名

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

2 Images

Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (AB8211)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (AB8211)

ab8211 staining alpha smooth muscle Actin in wild-type HeLa cells (top panel) and ACTA2 knockout HeLa cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8211 at 5μg/ml concentration and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor^® 647) at 1/100 dilution overnight at 4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (AB8211)

ab8211 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8211 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 4% formaldehyde (10 min).

不同偶联物与剂型 (6)

Unconjugated

Anti-alpha smooth muscle Actin antibody [1A4]
Carrier free

Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4]
Biotin

Biotin Anti-alpha smooth muscle Actin antibody [1A4]
HRP

HRP Anti-alpha smooth muscle Actin antibody [1A4]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

1A4

亚型

IgG2a

轻链类型

kappa

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/131.58", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Snake": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Avoid freeze / thaw cycle|Store in the dark

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.

Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2

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文献 (71)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 72:3787-3802 PubMed37668710

2023

PD-L1-expressing cancer-associated fibroblasts induce tumor immunosuppression and contribute to poor clinical outcome in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kento Kawasaki,Kazuhiro Noma,Takuya Kato,Toshiaki Ohara,Shunsuke Tanabe,Yasushige Takeda,Hijiri Matsumoto,Seitaro Nishimura,Tomoyoshi Kunitomo,Masaaki Akai,Teruki Kobayashi,Noriyuki Nishiwaki,Hajime Kashima,Naoaki Maeda,Satoru Kikuchi,Hiroshi Tazawa,Yasuhiro Shirakawa,Toshiyoshi Fujiwara

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 41:2186-2194 PubMed37316467

2023

Aging and injury affect nuclear shape heterogeneity in tendon.

Applications

Unspecified application

Species

Unspecified reactive species

Yann Tinguely,Vivian Shi,Franka Klatte-Schulz,Georg N Duda,Benjamin R Freedman,David J Mooney

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23028 PubMed37310356

2023

LRRC8A anion channels modulate vascular reactivity via association with myosin phosphatase rho interacting protein.

Applications

Unspecified application

Species

Unspecified reactive species

Hyehun Choi,Michael R Miller,Hong-Ngan Nguyen,Jeffrey C Rohrbough,Stephen R Koch,Naoko Boatwright,Michael T Yarboro,Rajan Sah,W Hayes McDonald,J Jeffrey Reese,Ryan J Stark,Fred S Lamb

Cancers 15: PubMed37296933

2023

Conventional Cancer Therapies Can Accelerate Malignant Potential of Cancer Cells by Activating Cancer-Associated Fibroblasts in Esophageal Cancer Models.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Komoto,Kazuhiro Noma,Takuya Kato,Teruki Kobayashi,Noriyuki Nishiwaki,Toru Narusaka,Hiroaki Sato,Yuki Katsura,Hajime Kashima,Satoru Kikuchi,Toshiaki Ohara,Hiroshi Tazawa,Toshiyoshi Fujiwara

Communications biology 6:130 PubMed36721040

2023

A vein wall cell atlas of murine venous thrombosis determined by single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Elise DeRoo,Ting Zhou,Huan Yang,Amelia Stranz,Peter Henke,Bo Liu

Nature communications 13:6752 PubMed36347862

2022

GZMK CD8 T effector memory cells are associated with CD15 neutrophil abundance in non-metastatic colorectal tumors and predict poor clinical outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Tiberti,Carlotta Catozzi,Ottavio Croci,Mattia Ballerini,Danilo Cagnina,Chiara Soriani,Caterina Scirgolea,Zheng Gong,Jiatai He,Angeli D Macandog,Amir Nabinejad,Carina B Nava Lauson,Arianna Quinte',Giovanni Bertalot,Wanda L Petz,Simona P Ravenda,Valerio Licursi,Paola Paci,Marco Rasponi,Luca Rotta,Nicola Fazio,Guangwen Ren,Uberto Fumagalli-Romario,Martin H Schaefer,Stefano Campaner,Enrico Lugli,Luigi Nezi,Teresa Manzo

Genes 13: PubMed36292593

2022

Single Cell Sequencing Reveals Mechanisms of Persistent Truncus Arteriosus Formation after and Double Knockout in Cardiac Neural Crest Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyun Chen,Shen Song,Haobin Jiang,Hong Lian,Shengshou Hu

The Journal of experimental medicine 219: PubMed35980387

2022

HER2 drives lung fibrosis by activating a metastatic cancer signature in invasive lung fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Liu,Yan Geng,Jiurong Liang,Ana Lucia Coelho,Changfu Yao,Nan Deng,Yizhou Wang,Kristy Dai,Guanling Huang,Ting Xie,Ningshan Liu,Simon C Rowan,Forough Taghavifar,Vrishika Kulur,Zhenqiu Liu,Barry R Stripp,Cory M Hogaboam,Dianhua Jiang,Paul W Noble

Development (Cambridge, England) 149: PubMed35831953

2022

Understanding the development of oral epithelial organs through single cell transcriptomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Qianlin Ye,Arshia Bhojwani,Jimmy K Hu

Frontiers in bioengineering and biotechnology 10:902894 PubMed35832407

2022

Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ying Teng,Ming-Li Zou,Si-Yu Liu,Yuan Jia,Kai-Wen Zhang,Zheng-Dong Yuan,Jun-Jie Wu,Jun-Xing Ye,Shun Yu,Xia Li,Xiao-Jin Zhou,Feng-Lai Yuan

View all publications

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Abcam Product Promise

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alexa-fluor-594-alpha-smooth-muscle-actin-antibody-1a4-ab202368

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FITC荧光Anti-alpha smooth muscle Actin抗体[1A4]

FITC Anti-alpha smooth muscle Actin antibody [1A4]

Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (AB8211)

Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (AB8211)

不同偶联物与剂型 (6)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用

免疫原

反应性数据

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产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (71)

Abcam Product Promise

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Biotin Anti-alpha smooth muscle Actin antibody [1A4]

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