AB19170

FITC Anti-alpha 1 Antitrypsin 抗体

FITC Anti-alpha 1 Antitrypsin antibody

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(4 Publications)

Goat Polyclonal alpha 1 Antitrypsin antibody - conjugated to FITC. Suitable for ICC, Flow Cyt, ELISA, ICC/IF and reacts with Human samples. Cited in 4 publications.

查看别名

AAT, PI, PRO0684, PRO2209, SERPINA1, Alpha-1-antitrypsin, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

反应种属

Human

应用

ICC, ELISA, ICC/IF, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

By immunoelectrophoresis and ELISA this antibody reacts specifically with alpha 1 antitrypsin. This antibody may cross react with alpha 1 antitrypsin light chain from other species.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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AB118971

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab19170 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).

存储溶液

pH: 6.8 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.

Biological function summary

A1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.

Pathways

Alpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.

Alpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.. Short peptide from AAT. Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

See full target information SERPINA1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Thorax 75:321-330 PubMed31959730

2020

Activation of complement component 3 is associated with airways disease and pulmonary emphysema in alpha-1 antitrypsin deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Michael E O'Brien,Laura Fee,Niall Browne,Tomás P Carroll,Paula Meleady,Michael Henry,Karen McQuillan,Mark P Murphy,Mark Logan,Cormac McCarthy,Oliver J McElvaney,Emer P Reeves,Noel G McElvaney

Science translational medicine 6:217ra1 PubMed24382893

2014

The circulating proteinase inhibitor α-1 antitrypsin regulates neutrophil degranulation and autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

David A Bergin,Emer P Reeves,Killian Hurley,Rebecca Wolfe,Ramia Jameel,Sean Fitzgerald,Noel G McElvaney

The Journal of clinical investigation 120:4236-50 PubMed21060150

2010

α-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8.

Applications

ICC/IF, Flow Cyt

Species

Human, Human

David A Bergin,Emer P Reeves,Paula Meleady,Michael Henry,Oliver J McElvaney,Tomás P Carroll,Claire Condron,Sanjay H Chotirmall,Martin Clynes,Shane J O'Neill,Noel G McElvaney

Journal of immunology (Baltimore, Md. : 1950) 184:4538-46 PubMed20228200

2010

Evidence for unfolded protein response activation in monocytes from individuals with alpha-1 antitrypsin deficiency.

Applications

ICC/IF

Species

Human

Tomás P Carroll,Catherine M Greene,Catherine A O'Connor,Aine M Nolan,Shane J O'Neill,Noel G McElvaney

View all publications

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alexa-fluor-647-alpha-1-antitrypsin-antibody-epr9090-ab206735

(0 reviews)

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For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-alpha 1 Antitrypsin 抗体

FITC Anti-alpha 1 Antitrypsin antibody

关键信息

宿主种属

克隆

亚型

偶联物

激发波长/发射波长

不含载体蛋白

反应种属

应用

免疫原

特异性

反应性数据

您可能对以下产品感兴趣:

Neutrophil Elastase Inhibitor Assay Kit

为什么推荐这个？

性能和储存信息

形式

纯化工艺

纯化说明

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (4)

Abcam Product Promise

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