重组FITC荧光Anti-6X His tag® [EPR20547] (ab318311)
![Flow Cytometry (Intracellular) - FITC Anti-6X His tag® [EPR20547] (ab318311)](https://www.abcam.cn/ps/products/318/ab318311/Images/ab318311-544798-ab318311anti6xhistagepr20547fitchek293tmychishsnai1flowcytometrynew.jpg "Flow Cytometry (Intracellular) - FITC Anti-6X His tag® [EPR20547] (ab318311)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- FITC Rabbit monoclonal [EPR20547] to 6X His tag® - ChIP Grade
- Suitable for: Flow Cyt (Intra)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-6X His tag® [EPR20547]
参阅全部 6X His tag® 一抗 -
描述
FITC荧光兔单克隆抗体[EPR20547] to 6X His tag® - ChIP Grade -
宿主
Rabbit -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Flow cyt (Intra): HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HEK293T-Myc-His-hSNAI1 vector
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20547 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)
- Anti-6X His tag® antibody [EPR20547] - BSA and Azide free (ab232492)
- Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547] (ab237336)
- Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (ab237337)
- PE Anti-6X His tag® antibody [EPR20547] (ab237338)
- HRP Anti-6X His tag® antibody [EPR20547] (ab237339)
- Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab316173)
- APC Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab317659)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318311于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/500. |
靶标
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相关性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
细胞定位
Depends upon the localization of the parent protein tagged with hexahistidine. -
别名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
图片
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Flow Cytometry (Intracellular) - FITC Anti-6X His tag® [EPR20547] (ab318311)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with HEK293T-Myc-His-hSNAI1 vector labeling 6X His tag® with ab318311 (middle panel) at 1.0μg/ml (1/500) compared with a Rabbit IgG [EPR25A] FITC (left panel)
Gate is set between transfected and untransfected HEK-293T cells.
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![FITC Anti-6X His tag® [EPR20547] (ab318311)](https://www.abcam.cn/ps/products/318/ab318311/Images/ab318311-2-benefitsofrecombinantantibodies.jpg)
FITC Anti-6X His tag® [EPR20547] (ab318311)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab318311 尚未被引用在任何文献中。