AB1206

FITC荧光Anti-6X His tag®抗体

Name: FITC Anti-6X His tag®抗体 (ab1206)| Abcam中文官网
Brand: Abcam Limited
SKU: AB1206
Price: 5162 CNY
Availability: InStock
Rating: 5 (7 reviews)

FITC Anti-6X His tag® antibody

(7 Reviews)

(36 Publications)

Anti-6X His tag® antibody - FITC conjugated (ab1206) is a rabbit polyclonal antibody detecting 6X His tag® in Flow Cytometry, ELISA.

- Over 20 publications
- Trusted since 2002

查看别名

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

2 Images

Flow Cytometry - FITC Anti-6X His tag® antibody (AB1206)

Flow Cyt

AbReview15560****

Flow Cytometry - FITC Anti-6X His tag® antibody (AB1206)

ab1206 staining 6X His tag^® in human endometrial carcinoma cells by Flow Cytometry.

Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with a 6X His tag^® primary antibody for 1 hour at 4°C. ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag^®. The small peak to the right represents cells that bound the tested sample.

This image is courtesy of an anonymous Abreview

Flow Cyt

PubMed

Flow Cytometry - FITC Anti-6X His tag® antibody (AB1206)

Muc1-Bi-1 and Muc1-Bi-2 can recognize Muc1 positive tumor cells.

a). Flow cytometry analysis of Muc1-Bi-1 and Muc1-Bi-2 on Muc1-negative cell lines HePG2 and CHO and Muc1-positive cell lines, HT29. Red line indicates cells with no staining; green line indicates cells with only anti-His-FITC staining (ab1206); blue line indicates cells with positive control anti-Muc1 antibody; purple line indicates cells with Muc1-Bi-1 protein; orange line indicates cells with Muc1-Bi-2 protein.

Li et al PLoS One. 2018 Jan 22;13(1):e0191024. doi: 10.1371/journal.pone.0191024. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

偶联物

FITC

激发波长/发射波长

Ex: 495nm, Em: 519nm

不含载体蛋白

应用

ELISA, Flow Cyt

applications

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反应性数据

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产品详情

What is this antibody validated in?
FITC Anti-6X His tag® antibody (ab1206) is a rabbit polyclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), ELISA. Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trusted by the scientific community
Anti-6X His tag® FITC (ab1206) was first used in a scientific publication in 2002 and has been cited over 20 times in peer-reviewed journals.

Reviewed by scientists
Anti-6X His tag® FITC (ab1206) has over 5 independent reviews from customers.

HIS-TAG® is a trademark of EMD Biosciences, Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.

存储溶液

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information 6X His tag®

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文献 (36)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112519 PubMed40546950

2025

Programmable cell aggregation by a synthetic biosilicification approach.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wang,Jing Sun,Lei Zang,Huaxiong Yao,Lin Wang,Runtao Zhu,Jie Li,Simin Zeng,Hongting Tang,Teng Wang,Ji Liu,Bo Wang,Bo Li,Zhiyuan Liu,Zhuojun Dai

Frontiers in immunology 16:1448438 PubMed40181964

2025

Developing CAR-T/NK cells that target EphA2 for non-small cell lung cancer treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Seok Min Kim,Soo Yun Lee,Seo In Kim,Ji Yeong Bae,Jin Tae Hong,Seona Jo,Ji Hyun Kim,Hyo-Young Chung,Tae-Don Kim

ACS synthetic biology 12:3608-3622 PubMed38010428

2023

Hyperstable Synthetic Mini-Proteins as Effective Ligand Scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Paul L Blanchard,Brandon J Knick,Sarah A Whelan,Benjamin J Hackel

Frontiers in medicine 10:1167452 PubMed37425304

2023

Analysis between ABO blood group and clinical outcomes in COVID-19 patients and the potential mediating role of ACE2.

Applications

Unspecified application

Species

Unspecified reactive species

Xianfei Zeng,Hongyan Fan,Jinxin Kou,Dongxue Lu,Fang Huang,Xi Meng,Haiying Liu,Zhuo Li,Mei Tang,Jing Zhang,Nannan Liu,Xingbin Hu

Vaccines 10: PubMed36146579

2022

Human Fc-Conjugated Receptor Binding Domain-Based Recombinant Subunit Vaccines with Short Linker Induce Potent Neutralizing Antibodies against Multiple SARS-CoV-2 Variants.

Applications

Unspecified application

Species

Unspecified reactive species

Liqing Chen,Xiaoxiao Qi,Dan Liang,Guiqi Li,Xiaofang Peng,Xiaohui Li,Bixia Ke,Huanying Zheng,Zhongqiu Liu,Changwen Ke,Guochao Liao,Liang Liu,Qian Feng

Translational oncology 21:101424 PubMed35477065

2022

A novel multifunctional anti-PD-L1-CD16a-IL15 induces potent cancer cell killing in PD-L1-positive tumour cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yumei Li,Lingjun Wu,Yueying Liu,Siwen Ma,Biyi Huang,Xianjing Feng,Hui Wang

mAbs 14:2021601 PubMed35030983

2022

Computational design of a neutralizing antibody with picomolar binding affinity for all concerning SARS-CoV-2 variants.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Seong Jeong,Jeong Seok Cha,Insu Hwang,Uijin Kim,Jared Adolf-Bryfogle,Brian Coventry,Hyun-Soo Cho,Kyun-Do Kim,Byung-Ha Oh

International journal of molecular sciences 23: PubMed35054983

2022

Syndecan-4 Is a Key Facilitator of the SARS-CoV-2 Delta Variant's Superior Transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Anett Hudák,Gábor Veres,Annamária Letoha,László Szilák,Tamás Letoha

The Journal of experimental medicine 218: PubMed34709351

2021

HVEM structures and mutants reveal distinct functions of binding to LIGHT and BTLA/CD160.

Applications

Unspecified application

Species

Unspecified reactive species

Weifeng Liu,Ting-Fang Chou,Sarah C Garrett-Thomson,Goo-Young Seo,Elena Fedorov,Udupi A Ramagopal,Jeffrey B Bonanno,Qingyang Wang,Kenneth Kim,Scott J Garforth,Kiyokazu Kakugawa,Hilde Cheroutre,Mitchell Kronenberg,Steven C Almo

Molecular oncology 16:485-507 PubMed34694686

2021

Recombinant nanobody against MUC1 tandem repeats inhibits growth, invasion, metastasis, and vascularization of spontaneous mouse mammary tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Parnaz Merikhian,Behrad Darvishi,Neda Jalili,Mohammad Reza Esmailinejad,Azadeh Sharif Khatibi,Shima Moradi Kalbolandi,Malihe Salehi,Marjan Mosayebzadeh,Mahdieh Shokrollahi Barough,Keivan Majidzadeh-A,Fatemeh Yadegari,Fatemeh Rahbarizadeh,Leila Farahmand

View all publications

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FITC荧光Anti-6X His tag®抗体

FITC Anti-6X His tag® antibody

Flow Cytometry - FITC Anti-6X His tag® antibody (AB1206)

Flow Cytometry - FITC Anti-6X His tag® antibody (AB1206)