FITC荧光Anti-5-methylcytosine (5-mC)抗体[5MC-CD] (ab179898)
![Immunocytochemistry/ Immunofluorescence - FITC Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (ab179898)](https://www.abcam.cn/ps/products/179/ab179898/Images/ab179898-208432-anti-5-methylcytosine-5-mc-antibody-5mc-cd-fitc-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - FITC Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (ab179898)")
Key features and details
- FITC Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC)
- Suitable for: ICC/IF
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgM
Related conjugates and formulations
概述
-
产品名称
FITC荧光Anti-5-methylcytosine (5-mC)抗体[5MC-CD]
参阅全部 5-methylcytosine (5-mC) 一抗 -
描述
FITC荧光小鼠单克隆抗体[5MC-CD] to 5-methylcytosine (5-mC) -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine Serum Albumin (BSA).
-
阳性对照
- Mouse embryonic stem cells; Chlamidomonas me-1 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading... -
纯度
Purified IgM -
纯化说明
ab179898 is filter-sterilised. -
克隆
单克隆 -
克隆编号
5MC-CD -
同种型
IgM -
研究领域
相关产品
-
Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179898于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/100.
|
| 说明 |
|---|
|
ICC/IF
1/50 - 1/100. |
靶标
-
形式
The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application. -
别名
- 5 m C antibody
- 5 mC antibody
- 5 me C antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - FITC Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (ab179898)
Immunofluorescent analysis of Chlamidomonas me-1 cells, labeling 5-Methyl Cytosine with ab179898 at 1/50 dilution, followed by a FITC-conjugated secondary antibody (middle panel). Left: DAPI-stained cells. Right: Merged image. Chloroplast DNA is exclusively methylated in gamete cells (bottom row), as opposed to vegetative cells (top row).
-
![Immunocytochemistry/ Immunofluorescence - FITC Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (ab179898)](https://www.abcam.cn/ps/products/179/ab179898/Images/ab179898-208433-anti-5-methylcytosine-5-mc-antibody-5mc-cd-fitc-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - FITC Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (ab179898)Immunofluorescent analysis of mouse embryonic stem cells labeling 5-Methyl Cytosine with ab179898 at 1/50 dilution.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab179898 尚未被引用在任何文献中。