AB21176

Anti-Firefly Luciferase 抗体

Name: Firefly Luciferase抗体 (ab21176)| Abcam中文官网
Brand: Abcam Limited
SKU: AB21176
Price: 7407 CNY
Availability: InStock
Rating: 4 (19 reviews)

Anti-Firefly Luciferase antibody

(19 Reviews)

(91 Publications)

Anti-Firefly Luciferase antibody (ab21176) is a rabbit polyclonal antibody detecting Firefly Luciferase in Western Blot, ICC/IF. Suitable for Firefly.

- Over 80 publications
- Trusted since 2005

查看别名

Luciferin 4-monooxygenase, Luciferase

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

ICC/IF

AbReview10285****

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

ab21176 staining mouse mammary carcinoma cells by ICC/IF.

Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking with a commercial blocking agent. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. An Alexa-Fluor^® 555 conjugated goat anti-rabbit antibody was used as the secondary.

The image shows firefly luciferase (red) in mouse tumour cells and cell nuclei counterstained with Hoechst (blue).

This image is courtesy of an anonymous Abreview

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

ab21176 at 10 μg/mL staining Luciferase in transfected HEK-293 (Human epithelial cell line from embryonic kidney) cells by ICC/IF.

The cells were fixed with methanol and acetone. An FITC conjugated anti-Rabbit IgG was used as the secondary antibody.

Left panel : Un-transfected cells.

Right panel : Transfected cells.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

Immunocytochemical analysis analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells overexpressing luciferase labeling Luciferase with ab21176 at a concentration of 10 μg/mL. The secondary antibody was a Goat anti-Rabbit IgG, FITC conjugate.

ICC/IF

AbReview5843****

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

ab21176 at 1/200 dilution staining engineered adult rat stromal stem cells by ICC/IF.

The cells were fixed in 2% paraformaldehyde and 0.1% Triton X-100 was used for cell permeabilization (15 minutes incubation time). The cells were incubated with the antibody overnight at 4°C. The image shows Luciferase (green-upper right panel), counterstained cell nuclei (DAPI-blue-upper left panel), overlay (lower left panel) and a phase contrast image (lower right panel).

The image was taken with a confocal laser scanning microscope equipped with an additional laser differential Interference Contrast (DIC) mode.

This image is courtesy of an Abreview submitted by Dr Mal Niladri

Supplier Data

Western blot - Anti-Firefly Luciferase antibody (AB21176)

All lanes:

Western blot - Anti-Firefly Luciferase antibody (ab21176) at 1/1000 dilution

Lane 1:

Lysates from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells overexpressing luciferase

Lane 2:

Lysates from HEK-293T cells overexpressing luciferase with Luciferase Immunizing Peptide

Secondary

All lanes:

Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Firefly

应用

ICC/IF, WB

applications

免疫原

Native Full Length Protein corresponding to Firefly Luciferin 4-monooxygenase.

P08659

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Firefly": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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产品详情

Anti-Firefly Luciferase antibody (ab21176) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in human, mouse samples.

Anti-Firefly Luciferase antibody (ab21176) has been cited over 80 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Firefly Luciferase antibody (ab21176) has high sensitivity and specificity.

Anti-Firefly Luciferase antibody (ab21176) has 18 independent reviews from customers.

Anti-Firefly Luciferase antibody (ab21176) specifically detects Firefly Luciferase (UniProt ID: P08659; Molecular weight: 60kDa) and is sold in 100 µL selling sizes.

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性能和储存信息

形式

Liquid

纯度

IgG fraction

存储溶液

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Firefly luciferase also known as luc assay or luciferase protein is an enzyme that catalyzes the oxidation of luciferin resulting in bioluminescence. This protein has a molecular mass of approximately 61 kDa and is responsible for the characteristic glow of fireflies. The luciferase expression occurs mainly in the light-producing organs of fireflies such as in the insect Photinus pyralis where it plays an important role in light emission.

Biological function summary

Firefly luciferase converts chemical energy into light making it valuable for various biological applications as a reporter gene. It participates in the formation of a complex with its substrates luciferin and ATP resulting in the production of a photon. This enzyme is widely used in molecular and cellular biology experiments to monitor gene expression and study protein-protein interactions due to its luminescent properties.

Pathways

Firefly luciferase functions in the bioluminescent pathway involving the conversion of luciferin to oxyluciferin and light emission. It interfaces with ATP a universal energy carrier which highlights its integration into the cellular energy systems. Firefly luciferase is similar to other oxidative enzymes though it is not directly involved with metabolic pathways like the TCA cycle or glycolysis.

Researchers often utilize firefly luciferase as a tool in tumor studies or infectious disease models. Its luminescence aids in imaging and tracking biological processes in real-time. While firefly luciferase itself is not directly associated with specific diseases the use of firefly agents allows researchers to study disease outcomes assess therapeutic efficacy and understand gene expression profiles related to cancer and viral infections often in connection with proteins such as p53 in cancer research.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Produces green light with a wavelength of 562 nm.

See full target information Luciferin 4-monooxygenase

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文献 (91)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013487 PubMed40920817

2025

TRIM28 is a target for paramyxovirus V proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Gauthier Lieber,Florence Kwaschik,Marie Lork,Nora Schmidt,Benjamin G Hale

Nature communications 16:2069 PubMed40021653

2025

Translational error in mice increases with ageing in an organ-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Erik C Böttger,Harshitha Santhosh Kumar,Adrian Steiner,Emmanuel Sotirakis,Kader Thiam,Patricia Isnard Petit,Petra Seebeck,David P Wolfer,Dimitri Shcherbakov,Rashid Akbergenov

Cancer research 85:942-955 PubMed39700395

2024

Selenoprotein O Promotes Melanoma Metastasis and Regulates Mitochondrial Complex II Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Luiza Martins Nascentes Melo,Marie Sabatier,Vijayashree Ramesh,Krystina J Szylo,Cameron S Fraser,Alex Pon,Evann C Mitchell,Kelly A Servage,Gabriele Allies,Isa V Westedt,Feyza Cansiz,Jonathan Krystkiewicz,Andrea Kutritz,Dirk Schadendorf,Sean J Morrison,Jessalyn M Ubellacker,Anju Sreelatha,Alpaslan Tasdogan

International journal of biological sciences 20:6207-6221 PubMed39664574

2024

MicroRNA-495 Modulates Neuronal Layer Fate Determination by Targeting .

Applications

Unspecified application

Species

Unspecified reactive species

Yunli Pang,Xiangbin Ruan,Wei Liu,Lin Hou,Bin Yin,Pengcheng Shu,Xiaozhong Peng

Molecular therapy : the journal of the American Society of Gene Therapy 32:3558-3579 PubMed39033322

2024

Extracellular vesicle surface display enhances the therapeutic efficacy and safety profile of cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Migara Kavishka Jayasinghe,Yock Sin Lay,Dawn Xiao Tian Liu,Chang Yu Lee,Chang Gao,Brendon Zhijie Yeo,Faith Yuan Xin How,Rebecca Carissa Prajogo,Dong Van Hoang,Hong Anh Le,Thach Tuan Pham,Boya Peng,Cao Dai Phung,Daniel G Tenen,Minh T N Le

Journal of extracellular biology 3:e145 PubMed38939412

2024

Dysregulation of intercellular communication in vitro and in vivo via extracellular vesicles secreted by pancreatic duct adenocarcinoma cells and generated under the influence of the AG9 elastin peptide-conditioned microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Lise Nannan,Salomé Decombis,Christine Terryn,Sandra Audonnet,Jean Michel,Sylvie Brassart-Pasco,Willy Gsell,Uwe Himmelreich,Bertrand Brassart

The Journal of biological chemistry 300:107391 PubMed38777144

2024

Hierarchical and scaffolded phosphorylation of two degrons controls PER2 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Joel Celio Francisco,David M Virshup

Cell 187:2465-2484.e22 PubMed38701782

2024

Small-molecule-induced epigenetic rejuvenation promotes SREBP condensation and overcomes barriers to CNS myelin regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xuezhao Liu,Dazhuan Eric Xin,Xiaowen Zhong,Chuntao Zhao,Zhidan Li,Liguo Zhang,Adam J Dourson,Lindsay Lee,Shreya Mishra,Arman E Bayat,Eva Nicholson,William L Seibel,Bingfang Yan,Joel Mason,Bradley J Turner,David G Gonsalvez,William Ong,Sing Yian Chew,Balaram Ghosh,Sung Ok Yoon,Mei Xin,Zhigang He,Jason Tchieu,Michael Wegner,Klaus-Armin Nave,Robin J M Franklin,Ranjan Dutta,Bruce D Trapp,Ming Hu,Matthew A Smith,Michael P Jankowski,Samantha K Barton,Xuelian He,Q Richard Lu

Scientific reports 14:2616 PubMed38297007

2024

Craniofacial and olfactory sensory changes after long-term unilateral nasal obstruction-an animal study using MMP-3-LUC transgenic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Fang Hsu,Nutthakarn Ratanasereeprasert,Shih-Kai Wang,Jung-Tsu Chen,Yi-Jane Chen,Te-Huei Yeh,Hsiang-Hsuan Sung,Chung-Chen Jane Yao

Cells 12: PubMed36611917

2022

iNOS Deletion in Alveolar Epithelium Cannot Reverse the Elastase-Induced Emphysema in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marija Gredic,Vinita Sharma,Stefan Hadzic,Cheng-Yu Wu,Oleg Pak,Baktybek Kojonazarov,Julia Duerr,Marcus A Mall,Andreas Guenther,Ralph T Schermuly,Friedrich Grimminger,Werner Seeger,Simone Kraut,Natascha Sommer,Norbert Weissmann

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

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Anti-Firefly Luciferase 抗体

Anti-Firefly Luciferase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody (AB21176)

Western blot - Anti-Firefly Luciferase antibody (AB21176)