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AB313621

重组Anti-FIP200抗体[EPR26076-87] - BSA and Azide free

Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free

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Rabbit Recombinant Monoclonal FIP200 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

查看别名

KIAA0203, RBICC, RB1CC1, RB1-inducible coiled-coil protein 1, FAK family kinase-interacting protein of 200 kDa, FIP200

5 Images
Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)
  • WB

Supplier Data

Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)

This data was developed using ab313620, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 37 seconds

All lanes:

Western blot - Anti-FIP200 antibody [EPR26076-87] (<a href='/products/primary-antibodies/fip200-antibody-epr26076-87-ab313620'>ab313620</a>) at 1/1000 dilution

All lanes:

Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 200 kDa

false

Exposure time: 37s

Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)
  • WB

Supplier Data

Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)

This data was developed using ab313620, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-FIP200 antibody [EPR26076-87] (<a href='/products/primary-antibodies/fip200-antibody-epr26076-87-ab313620'>ab313620</a>) at 1/1000 dilution

All lanes:

HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 200 kDa

true

Exposure time: 180s

Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)
  • WB

Supplier Data

Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)

This data was developed using ab313620, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-FIP200 antibody [EPR26076-87] (<a href='/products/primary-antibodies/fip200-antibody-epr26076-87-ab313620'>ab313620</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

HeLa treated with 2uM bafilomycin A1 for 18h whole cell lysate at 50 µg

Lane 3:

Untreated MEF (mouse embryo fibroblast) whole cell lysate at 50 µg

Lane 4:

MEF treated with 2uM bafilomycin A1 for 18h whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 200 kDa

false

Exposure time: 180s

Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)
  • WB

Supplier Data

Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)

This data was developed using ab313620, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

The identity of the lower MW bands at approximately 100 kDa are unknown.

In Western blot, anti- Vinculin antibody (ab129002) loading control staining at 1/10000 dilution.

All lanes:

Western blot - Anti-FIP200 antibody [EPR26076-87] (<a href='/products/primary-antibodies/fip200-antibody-epr26076-87-ab313620'>ab313620</a>) at 1/1000 dilution

Lane 1:

Untreated A-172 (human brain glioblastoma ) whole cell lysate at 50 µg

Lane 2:

A-172 treated with 50uM chloroquine for 8h whole cell lysate at 50 µg

Lane 3:

Untreated C2C12 (mouse myoblast) whole cell lysate at 50 µg

Lane 4:

C2C12 treated with 50uM chloroquine for 24h whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 200 kDa

true

Exposure time: 180s

Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)
  • WB

Supplier Data

Western blot - Anti-FIP200 antibody [EPR26076-87] - BSA and Azide free (AB313621)

This data was developed using ab313620, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 10 seconds

All lanes:

Western blot - Anti-FIP200 antibody [EPR26076-87] (<a href='/products/primary-antibodies/fip200-antibody-epr26076-87-ab313620'>ab313620</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 50 µg

Lane 2:

Rat testis tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 200 kDa

false

Exposure time: 10s

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR26076-87

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse, Rat

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

FIP200 also known as RB1CC1 is a large protein with a mass of approximately 200 kDa. It functions in several cellular processes acting as a scaffold protein that assists in the assembly of different protein complexes. FIP200 is widely expressed in human tissues including the brain heart and skeletal muscle. This suggests it has important roles in diverse biological functions within the body.
Biological function summary

FIP200 is an important component of the ULK1 complex which is a critical player in the regulation of autophagy. Autophagy is a cellular degradation pathway that removes damaged organelles and proteins helping maintain cellular homeostasis. FIP200 interacts with other proteins such as ULK1 and ATG13 to facilitate the initiation of autophagy. Its role in this complex ensures proper cellular response to nutrient deprivation and stress conditions.

Pathways

The mechanistic functions of FIP200 integrate into the autophagy and mTOR signaling pathways. In the mTOR pathway FIP200 acts upstream interacting with proteins like mTOR kinase to coordinate cell growth and autophagy. This interaction contributes to maintaining cellular energy levels and adaptions to metabolic stress. FIP200's regulatory activities within these pathways impact various cellular functions aligning cell metabolism and survival mechanisms.

FIP200's dysfunction has connections to cancer and neurodegenerative diseases. In cancer alterations in FIP200 expression can affect cell proliferation and tumorigenesis possibly through associations with proteins like p53. In neurodegenerative diseases improper autophagy due to malfunctioning FIP200 can lead to the accumulation of damaged proteins and cellular stress forging a link with disorders such as Alzheimer's. Understanding FIP200’s role in these conditions aids the exploration of therapeutic targets for disease treatment.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Involved in autophagy (PubMed : 21775823). Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1 (PubMed : 23392225). Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (By similarity). Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways (PubMed : 10769033, PubMed : 12221124). Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (By similarity). Functions as a DNA-binding transcription factor (PubMed : 12095676). Is a potent regulator of the RB1 pathway through induction of RB1 expression (PubMed : 14533007). Plays a crucial role in muscular differentiation (PubMed : 12163359). Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity).
See full target information RB1CC1
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搭配使用产品

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

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Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

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Primary Antibodies

AB109364

Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB109364)

  • 4
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Primary Antibodies

AB105459

Anti-WIPI2 antibody [2A2]

BOND RX™ Validated|KO Validated|Trial Size|Trial Size

Western blot - Anti-WIPI2 antibody [2A2] (AB105459)

  • 5
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