Anti-FIP1L1抗体(ab80271)
Key features and details
- Rabbit polyclonal to FIP1L1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FIP1L1抗体
参阅全部 FIP1L1 一抗 -
描述
兔多克隆抗体to FIP1L1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Cow, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
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免疫原
Synthetic peptide within Human FIP1L1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_112179.2
Database link: Q6UN15 -
阳性对照
- Whole cell lysate from HeLa and 293T cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80271于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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相关性
FIP1L1 is an integral subunit of the cleavage and polyadenylation specificity factor (CPSF) complex. CPSF is a multisubunit complex that binds to the canonical AAUAAA hexamer and to U-rich upstream sequence elements on the pre-mRNA and interacts with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 binds to U-rich RNA sequence elements surrounding the poly(A) site and contributes to poly(A) site recognition and stimulates poly(A) addition. FIP1L1 may act to tether poly(A) polymerase to the CPSF complex. A fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA) is a recurrent molecular lesion in eosinophilia-associated myeloproliferative disorders. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 81608 Human
- Entrez Gene: 100172699 Orangutan
- Omim: 607686 Human
- SwissProt: Q6UN15 Human
- SwissProt: Q5RAA7 Orangutan
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别名
- DKFZp586K0717 antibody
- Factor interacting with PAP antibody
- FIP1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIP1L1 antibody (ab80271)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic islet cell tumor tissue labelling FIP1L1 with ab80271 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-FIP1L1 antibody (ab80271)All lanes : Anti-FIP1L1 antibody (ab80271) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 67 kDa
Observed band size: 67 kDa -
Immunoprecipitation - Anti-FIP1L1 antibody (ab80271)Detection of FIP1L1 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80271 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab80271 尚未被引用在任何文献中。