Anti-FILGAP抗体(ab203874)
Key features and details
- Rabbit polyclonal to FILGAP
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-FILGAP抗体
参阅全部 FILGAP 一抗 -
描述
兔多克隆抗体to FILGAP -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse
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免疫原
Synthetic peptide within Human FILGAP aa 600-700 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8N264 -
阳性对照
- Human lung carcinoma tissue; Rat kidney lysate; Human colon carcinoma lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab203874于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/500.
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| WB |
1/100 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
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| 说明 |
|---|
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IHC-P
1/100 - 1/500. |
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WB
1/100 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa). |
靶标
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相关性
ARHGAP24 is a rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. It acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. It controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. There are five different isoforms.Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells and is involved in modulation of angiogenesis. -
细胞定位
Cytoplasmic, cytoskeleton. Cell junction: adherens junction, focal adhesion. Cell projection. -
数据库链接
- Entrez Gene: 83478 Human
- Entrez Gene: 231532 Mouse
- Entrez Gene: 305156 Rat
- Omim: 610586 Human
- SwissProt: Q8N264 Human
- SwissProt: Q8C4V1 Mouse
- SwissProt: Q5U2Z7 Rat
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别名
- ARHGAP24 antibody
- DKFZP564B1162 antibody
- Filamin A associated RhoGAP antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FILGAP antibody (ab203874)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling FILGAP with ab203874 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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Western blot - Anti-FILGAP antibody (ab203874)All lanes : Anti-FILGAP antibody (ab203874) at 1/200 dilution
Lane 1 : Human colon carcinoma lysate
Lane 2 : Rat kidney lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 84 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab203874 被引用在 6 文献中.
- Yokoyama T et al. Smad2Δexon3 and Smad3 have distinct properties in signal transmission leading to TGF-β-induced cell motility. J Biol Chem 299:102820 (2023). PubMed: 36549646
- Yang C et al. Transcriptomic Analysis Identified ARHGAP Family as a Novel Biomarker Associated With Tumor-Promoting Immune Infiltration and Nanomechanical Characteristics in Bladder Cancer. Front Cell Dev Biol 9:657219 (2021). PubMed: 34307347
- Wang L et al. Rho GTPase Activating Protein 24 (ARHGAP24) Silencing Promotes Lung Cancer Cell Migration and Invasion by Activating ß-Catenin Signaling. Med Sci Monit 25:21-31 (2019). PubMed: 30599132
- Wang L et al. ARHGAP24 inhibits cell proliferation and cell cycle progression and induces apoptosis of lung cancer via a STAT6-WWP2-P27 axis. Carcinogenesis N/A:N/A (2019). PubMed: 31430374
- Zhang S et al. ARHGAP24 regulates cell ability and apoptosis of colorectal cancer cells via the regulation of P53. Oncol Lett 16:3517-3524 (2018). PubMed: 30127956
- Dai X et al. Rho GTPase Activating Protein 24 (ARHGAP24) Regulates the Anti-Cancer Activity of Sorafenib Against Breast Cancer MDA-MB-231 Cells via the Signal Transducer and Activator of Transcription 3 (STAT3) Signaling Pathway. Med Sci Monit 24:8669-8677 (2018). PubMed: 30499465