重组Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107)抗体[EP2310AY] (ab75978)

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure time 5.5 seconds

Immunohistochemistry analysis of Paraffin-embedded mouse kidney sections labeling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/200 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Positive staining on mouse kidney without alkaline phosphatase treatment (image A); No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab75978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunohistochemistry analysis of Paraffin-embedded rat kidney sections labeling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/200 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Positive staining on rat kidney without alkaline phosphatase treatment (image A); No signal was detected when tissues were treated with alkaline phosphatase (image B).
The section was incubated with ab75978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Dot blot analysis of Filamin A (phospho S2152) + Filamin B (phospho S2107) using ab75978 at 1/1000 followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Lane 1: Filamin A non-phospho peptide
Lane 2: Filamin A (phospho S2152) peptide a
Lane 3: Filamin A (phospho S2158) peptide b
Lane 4: Filamin B non-phospho peptide
Lane 5: Filamin B (phospho S2107) peptide a
Lane 6: Filamin B (phospho S2113) peptide b
Lane 7: Filamin C non-phospho peptide
Lane 8: Filamin C (phospho S2146) peptide a
Lane 9: Filamin C (phospho S2152) peptide b

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This antibody does cross-react with Filamin A (phospho S2152) and Filamin B (phospho S2107).

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Immunohistochemistry analysis of Paraffin-embedded human cardiac muscle sections labeling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/200 dilution followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Negative control: No staining on the intercalated disks in human cardiac muscle, which shows ab75978 doesn't react with Filamin C.
The section was incubated with ab75978 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/1200 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human kidney tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/1200 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse kidney tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/1200 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Rat kidney tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.