Anti-Filaggrin抗体(ab81468)
Key features and details
- Rabbit polyclonal to Filaggrin
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Filaggrin抗体
参阅全部 Filaggrin 一抗 -
描述
兔多克隆抗体to Filaggrin -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Filaggrin (N terminal).
Database link: P20930 -
常规说明
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR309720-5, GR3178284-1, GR309720-6, GR309720-2, GR309720-3, GR309720-4. Lot numbers other than GR309720-5, GR3178284-1, GR309720-6, GR309720-2, GR309720-3, GR309720-4 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81468于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
靶标
-
功能
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. -
组织特异性
Keratohyalin granules. -
疾病相关
Ichthyosis vulgaris
Dermatitis atopic 2 -
序列相似性
Belongs to the S100-fused protein family.
Contains 2 EF-hand domains.
Contains 23 filaggrin repeats. -
翻译后修饰
Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
Undergoes deimination of some arginine residues (citrullination). - Information by UniProt
-
数据库链接
- Entrez Gene: 2312 Human
- Omim: 135940 Human
- SwissProt: P20930 Human
- Unigene: 654510 Human
-
别名
- ATOD2 antibody
- Epidermal filaggrin antibody
- FILA_HUMAN antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-Filaggrin antibody (ab81468)
ICC/IF image of ab81468 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81468, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (35)
ab81468 被引用在 35 文献中.
- Chai RJ et al. Developing a bioink for single-step deposition and maturation of human epidermis. Int J Bioprint 9:738 (2023). PubMed: 37323493
- Inan S et al. Epicutaneous Sensitization to the Phytocannabinoid β-Caryophyllene Induces Pruritic Inflammation. Int J Mol Sci 24:N/A (2023). PubMed: 37762646
- Zhang J et al. Bioinspired polydopamine nanoparticles as efficient antioxidative and anti-inflammatory enhancers against UV-induced skin damage. J Nanobiotechnology 21:354 (2023). PubMed: 37775761
- Reuter C et al. Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms. Nat Commun 14:7660 (2023). PubMed: 37996412
- Kok JML et al. Macrocystis pyrifera Lipids Reduce Cytokine-Induced Pro-Inflammatory Signalling and Barrier Dysfunction in Human Keratinocyte Models. Int J Mol Sci 24:N/A (2023). PubMed: 38003573