Anti-Fibronectin抗体[IST-9] - BSA and Azide free (ab6328)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [IST-9] - BSA and Azide free (ab6328)](https://www.abcam.cn/ps/products/6/ab6328/Images/ab6328-319652-anti-fibronectin-antibody-ist-9-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [IST-9] - BSA and Azide free (ab6328)")
Key features and details
- Mouse monoclonal [IST-9] to Fibronectin - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Rat, Dog, Human
- Isotype: IgG1
概述
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产品名称
Anti-Fibronectin抗体[IST-9] - BSA and Azide free
参阅全部 Fibronectin 一抗 -
描述
小鼠单克隆抗体[IST-9] to Fibronectin - BSA and Azide free -
宿主
Mouse -
特异性
This antibody reacts with an epitope (PEDGIHELFP) located in the ED-A sequence of cellular fibronectin (it therefore only detects the cFN and not extracellular FN (plasma FN)). (Liao et al.) The ED-A segment can be included or omitted from the molecule depending on the pattern of splicing of the mRNA precursors. In transformed or tumour derived cells the ED-A segment is about 10-times higher than in FN from normal fibroblasts, and it may therefore be a significant marker for malignancy (Borsi et al., and others). -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Rat, Dog, Human
预测可用于: Mouse, Rabbit
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免疫原
Recombinant fragment corresponding to Human Fibronectin aa 1631-1721. Sequence of the type III repeats termed EIIIA (or ED-A).
Sequence:NIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAP DGEEDTAELQ GLRPGSEYTVSVVALHDDMESQPLIGTQSTA
Database link: P02751 -
表位
Liao et al: Clone IST-9, binds to the Ile43 and His44 residues within ED-A in a conformationally dependent fashion, implicating the loop region encompassing both residues as critical for mediating ED-A function. The conformational domain C-C'-E of rat EIIIA encompassing the His44 residue is crucial for constituting the IST-9 epitope. -
阳性对照
- IHC-P: Canine myocardial infarct. tissue. Rat kidney tissue. Human small intestine tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
IST-9 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (18) |
1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
See AbreviewsConcentration of IST-9 in IHC-P is - depending on the system - 2-10 micrograms per ml. |
| 说明 |
|---|
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IHC-P
1/100 - 1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. See AbreviewsConcentration of IST-9 in IHC-P is - depending on the system - 2-10 micrograms per ml. |
靶标
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功能
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
组织特异性
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
疾病相关
Glomerulopathy with fibronectin deposits 2 -
序列相似性
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
发展阶段
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
翻译后修饰
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
细胞定位
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 2335 Human
- Entrez Gene: 14268 Mouse
- Entrez Gene: 25661 Rat
- Omim: 135600 Human
- SwissProt: P02751 Human
- SwissProt: P11276 Mouse
- SwissProt: P04937 Rat
- Unigene: 203717 Human
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别名
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [IST-9] - BSA and Azide free (ab6328)Amann et al PLoS One. 2014 Mar 24;9(3):e92511. doi: 10.1371/journal.pone.0092511. eCollection 2014. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Extracellular matrix expression pattern.
Fibronectin was displayed in microtissues after ten days. (Bar in A549/SV80 monocultures: 50 μm, bar in all other microtissues: 100 μm); Secretion of fibronectin could be observed in microtissues containing fibroblasts, whereas in all tumour cell monocultures no fibronectin secretion could be detected.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [IST-9] - BSA and Azide free (ab6328)](https://www.abcam.cn/ps/products/6/ab6328/Images/ab6328-12275-anti-fibronectin-antibody-ist-9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [IST-9] - BSA and Azide free (ab6328)This image is courtesy of an Abreview submitted by Marcin Dobaczewskiab6328 positively staining paraffin fixed canine myocardial infarct. tissue (1/200). The images demonstrate the time course of fibronectin deposition in reperfused canine myocaridal infarcts.
a: 24 hrs
b: 7 days
c: 28 days of reperfusion.
Secondary: Biotin conjugated goat anti mouse. Detection was achieved using DAB. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [IST-9] - BSA and Azide free (ab6328)](https://www.abcam.cn/ps/products/6/ab6328/Images/ab6328-12276-anti-fibronectin-antibody-ist-9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [IST-9] - BSA and Azide free (ab6328)This image is courtesy of an Abreview submitted by Elizabeth ChlipalaFormalin fixed paraffin embedded rat kidney stained with ab6328 at a dilution of 1:100 after proteinase K digestion.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [IST-9] - BSA and Azide free (ab6328)](https://www.abcam.cn/ps/products/6/ab6328/Images/ab6328-168803-anti-fibronectin-antibody-ist-9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [IST-9] - BSA and Azide free (ab6328)This image is courtesy of an Abreview submitted by Rudolf Jungab6328 staining Fibronectin in human small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a Tris-EDTA buffer pH 9.0. Samples were incubated with primary antibody (1/50 in blocking buffer) for 30 minutes at 20°C. An undiluted HRP-conjugated Goat anti-mouse polyclonal was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (244)
ab6328 被引用在 244 文献中.
- Borges-Vélez G et al. Zika virus infection of the placenta alters extracellular matrix proteome. J Mol Histol 53:199-214 (2022). PubMed: 34264436
- Zhu X et al. Fabrication of Size-Controllable and Arrangement-Orderly HepG2 Spheroids for Drug Screening via Decellularized Liver Matrix-Derived Micropattern Array Chips. ACS Omega 7:2364-2376 (2022). PubMed: 35071924
- Witt J et al. Cryopreservation in a Standard Freezer: -28 °C as Alternative Storage Temperature for Amniotic Membrane Transplantation. J Clin Med 11:N/A (2022). PubMed: 35207382
- Yuan D et al. Knockdown of RSPH14 inhibits proliferation, migration, and invasion and promotes apoptosis of hepatocellular carcinoma via RelA. Cancer Cell Int 22:129 (2022). PubMed: 35305640
- Zakharova I et al. Mitomycin-Treated Endothelial and Smooth Muscle Cells Suitable for Safe Tissue Engineering Approaches. Front Bioeng Biotechnol 10:772981 (2022). PubMed: 35360387
