Anti-Fibronectin抗体[Fn-3] (ab18265)
![Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (ab18265)](https://www.abcam.cn/ps/products/18/ab18265/Images/ab18265-27924-anti-fibronectin-antibody-fn-3-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (ab18265)")
Key features and details
- Mouse monoclonal [Fn-3] to Fibronectin
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Fibronectin抗体[Fn-3]
参阅全部 Fibronectin 一抗 -
描述
小鼠单克隆抗体[Fn-3] to Fibronectin -
宿主
Mouse -
特异性
Reacts with human cellular fibronectin, but not plasma fibronectin. -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Fibronectin. FR5 cells, derived by SV40 transformation of human mammary epithelial cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
Fn-3 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18265于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
组织特异性
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
疾病相关
Glomerulopathy with fibronectin deposits 2 -
序列相似性
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
发展阶段
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
翻译后修饰
Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
细胞定位
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 2335 Human
- Omim: 135600 Human
- SwissProt: P02751 Human
- Unigene: 203717 Human
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别名
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (ab18265)
ICC/IF image of ab18265 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab18265 被引用在 10 文献中.
- Hu J et al. Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy. J Immunother Cancer 10:N/A (2022). PubMed: 35236741
- Shih CC et al. Protective Effects of One 2,4-Dihydro-3H-Pyrazol-3-one Derivative against Posterior Capsular Opacification by Regulation of TGF-β2/SMADs and Non-SMAD Signaling, Collagen I, and Fibronectin Proteins. Curr Issues Mol Biol 44:5048-5066 (2022). PubMed: 36286058
- Wei M et al. Genipin Induces Autophagy and Suppresses Cell Growth of Oral Squamous Cell Carcinoma via PI3K/AKT/MTOR Pathway. Drug Des Devel Ther 14:395-405 (2020). PubMed: 32099325
- Li Y et al. Retracted Article: Long non-coding RNA TUG1 alleviates high glucose induced podocyte inflammation, fibrosis and apoptosis in diabetic nephropathy via targeting the miR-27a-3p/E2F3 axis. RSC Adv 9:37620-37629 (2019). PubMed: 35542278
- Wang J et al. Long noncoding RNA homeobox A11 antisense promotes transforming growth factor ß1-induced fibrogenesis in cardiac fibroblasts. Mol Med Rep 19:2817-2824 (2019). PubMed: 30720066
- Gan L et al. Long non-coding RNA H19 regulates cell growth and metastasis via the miR-22-3p/Snail1 axis in gastric cancer. Int J Oncol 54:2157-2168 (2019). PubMed: 31081061
- Wong CH et al. Bio-functionalized magnetic nanoparticles for the immunoassay of fetal fibronectin: a feasibility study for the prediction of preterm birth. Sci Rep 7:42461 (2017). PubMed: 28198381
- Bambang IF et al. Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells. Oncogene : (2012). WB . PubMed: 22543584
- Cseh B et al. Autocrine fibronectin directs matrix assembly and crosstalk between cell-matrix and cell-cell adhesion in vascular endothelial cells. J Cell Sci 123:3989-99 (2010). ICC/IF ; Cow . PubMed: 20980391
- Keen J et al. Monoclonal antibodies that distinguish between human cellular and plasma fibronectin. Mol Biol Med 2:15-27 (1984). PubMed: 6083431