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重组RabMAb

重组Anti-Fibroblast activation蛋白，alpha抗体[EPR27358-9] - BSA and Azide free (ab317556)

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Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)
  • Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)
  • Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)
  • Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR27358-9] to Fibroblast activation protein, alpha - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Fibroblast activation蛋白，alpha抗体[EPR27358-9] - BSA and Azide free
    参阅全部 Fibroblast activation protein, alpha 一抗

  • 描述

    兔单克隆抗体[EPR27358-9] to Fibroblast activation蛋白，alpha - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cytmore details
    不适用于: ICC/IF,IHC-P,IP or WB

  • 种属反应性

    与反应: Mouse, Human
    不与反应: Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Flow Cyt: MEF, C2C12, WI-38 cells.

  • 常规说明

    ab317556 is the carrier-free version of ab317555.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317556于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use at an assay dependent concentration.
说明
Flow Cyt
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF,IHC-P,IP or WB.

靶标

  • 功能

    In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

  • 组织特异性

    Fibroblast specific.

  • 序列相似性

    Belongs to the peptidase S9B family.

  • 翻译后修饰

    N-glycosylated.
    The N-terminus may be blocked.

  • 细胞定位

    Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 170 kDa melanoma membrane bound gelatinase antibody
      • 170 kDa melanoma membrane-bound gelatinase antibody
      • DPPIV antibody
      • FAP antibody
      • FAPA antibody
      • Fibroblast activation protein alpha antibody
      • Integral membrane serine protease antibody
      • SEPR_HUMAN antibody
      • Seprase antibody
      see all

    图片

    • Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)
      Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)

      This data was developed using ab317555, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of MEF(mouse embryo fibroblast, Top left) / C2C12(mouse myoblast, Top right) / RAW 264.7(mouse Abelson murine leukemia virus-induced tumor macrophage, Bottom left) / EL4(mouse lymphoma T lymphocyte, Bottom right) cells labelling Fibroblast activation protein, alpha with ab317555 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Gated on viable cell.
      Negative control: RAW 264.7(PMID: 512331) and EL4(PMID: 16322266).

    • Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)
      Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)

      This data was developed using ab317555, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Jurkat(human T cell leukemia T lymphocyte from peripheral blood, Left) / WI-38(human fetal lung fibroblast, Right) cells labelling Fibroblast activation protein, alpha with ab317555 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

      Gated on viable cell.
      Negative control: Jurkat.

    • Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)
      Flow Cytometry - Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)

      This data was developed using ab317555, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of Isotype (Left) / 293T cells transfected with a Fibroblast activation protein, alpha expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling Fibroblast activation protein, alpha with ab317555 at 1/5000 dilution (0.01 ug)/Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

      Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

    • Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)
      Anti-Fibroblast activation protein, alpha antibody [EPR27358-9] - BSA and Azide free (ab317556)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab317556？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab317556 尚未被引用在任何文献中。

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