Anti-Fibroblast activation蛋白，alpha抗体(ab28244)

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, I would like to collect a few more details from you in regards to the samples and protocol used with the antibody. To this end, I have attached aquestionnaire. This should only take 5-10 minutes to complete and if you could include any images of the staining that would be very helpful.

OnceI havereceive the completed questionnaire,I will look at the protocol and see if there are any suggestionsI can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary.

If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Thank you for contacting Abcam.

I would recommend using ab28244 or ab28246 as each of these is guaranteed to work in IF on rat tissues under our Abpromise.

The immunogen for ab28244 Fibroblast activation protein, alpha antibody starts at the beginning of the stalk region.This region starts at amino acid 30 of the 760 amino acid sequence of human FAP-alpha, and follows the transmembrane domain. Cleavage of the stalk region releases the shed form of FAP-alpha.

ab28246(Fibroblast activation protein, alpha antibody - Catalytic insert) will recognize the 759 amino acid sequence of human FAP-alpha which has an insert in the catalytic domain.

These products are covered by our Abpromise guarantee. If you experience any issues with these products we offer scientific support, replacement or refund under our Abpromise guarantee, for a period of 6 months from purchase, if used according to specifications listed on our datasheet.

Thank you for contacting Abcam.
We do not know of any publications which have used this product in IHC-P in mouse tissues.
A good starting point for determining the optimal concentration is to use the dilution used in the earlier referenced paper, 1:600. Of course you will need to titer out the best concentration but using this reference for your experiments will give you an excellent start.
I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for contacting us.

This product was used in IHC-P in the following publication: Bhati R et al. Molecular characterization of human breast tumor vascular cells. Am J Pathol 172:1381-90 (2008). IHC-P; Human. (PubMed: 18403594) (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2329846/?tool=pubmed)
Where it is referenced as Rabbit polyclonal antibody to fibroblast activation protein (FAP), α-Stalk region (Abcam, Cambridge, MA) and was used at 1:600 dilution.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry and your interest in our products.

The tested applications and the species are normally listed on the on-line product datasheets. Generally, we test our antibodies in human, mouse and rat samples - very few antibodies are characterized in specifically in porcine. However, it does not necessary mean that they are unsuitable in this species but we have no preliminary data yet to support the cross-reactivity.

I have conducted a search for you and found some antibodies (ab28244, ab44971, ab51824) which recognize fibroblasts and are suitable for Flow cytometry but no cross-reactivity data with porcine so far. We can certainly offer a discount code for testings and the Terms and Conditions of this offer can be found at the following site: https://www.abcam.com/collaborationdiscount.

If you are interested in any particular fibroblast markers, you may wish to conduct a search using our website and check some publications using PubMed websites.

I hope this helps and if I can assist further, please do not hesitate to contact me.

Thank you for contacting Abcam regarding ab28244. Unfortunately the immunogen information for ab28244 is considered proprietary and I do not have access to that information. Please accept my apologies for not being able to provide you with the information you required. If there is anything else I can do help you, please do not hesitate to contact me.

Thank you for your reply. I can confirm that only ab28244 (Anti-Fibroblast activation protein, alpha antibody - Stalk region) is tested and guaranteed for flow cytometry and I therefore recommend this antibody for your experiments. Please find a publication that used this antibody in flow cytometry on the datasheet: datasheet=28244.https://www.abcam.com/Fibroblast-activation-protein-alpha-antibody-ab28244.html https://www.abcam.com/Fibroblast-activation-protein-alpha-antibody-ab28244.html. The descriptive terms for these antibodies are based on comparison with the dipeptidyl peptidase family, especially DPP-4. FAP-alpha starts with a short cytoplasmic domain followed by a transmembrane domain. The stalk region, which starts at amino acid 30 of the 760 amino acid sequence of human FAP-alpha, follows the transmembrane domain and is extracellular. Cleavage of the stalk region releases the shed form of FAP-alpha. RP1-FAP-alpha (ab28244 Fibroblast activation protein, alpha antibody - Stalk region) starts at the beginning of the stalk region. This antibody recognizes the Stalk Region of both long isoforms of Fibroblast activation protein-alpha. Between the stalk region and the catalytic domain is the spacer region. RP2-FAP-alpha ab28245 (Fibroblast activation protein, alpha antibody - Spacer region) is in the middle of this spacer region, it starts shortly after amino acid 250. I hope this will be helpful to you. Please fell for to contact me again in case you have further questions.

Thank you for contacting us. I can confirm that ab28244 is tested and guaranteed for FACS and IHC-P in mouse, human and rat by our Abpromise. and therefore should be suitable to isolate cells that express FAP. This antibody recognizes the Stalk Region of both long isoforms of Fibroblast activation protein-alpha. The stalk region, which starts at amino acid 30 of the 760 amino acid sequence of human FAP-alpha, follows the transmembrane domain and is extracellular. Cleavage of the stalk region releases the shed form of FAP-alpha. We have no data concerning the recognition of the cleaved FAP by ab28244. We are not specialized in the field of pancreatic stellate cells and do not know if this antibody is suitable to isolate these cells specifically. We do not want to recommend a product that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests. I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.