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Anti-Fibrinogen gamma chain抗体[5A6] (ab119948)

Reviews (1) Submit a question References (9)
Western blot - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
  • ELISA - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)

Key features and details

  • Mouse monoclonal [5A6] to Fibrinogen gamma chain
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

概述

  • 产品名称

    Anti-Fibrinogen gamma chain抗体[5A6]
    参阅全部 Fibrinogen gamma chain 一抗

  • 描述

    小鼠单克隆抗体[5A6] to Fibrinogen gamma chain

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ELISA, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Recombinant fragment, corresponding to amino acids 210-437 of Human Fibrinogen gamma chain

  • 阳性对照

    • Recombinant Fibrinogen gamma chain protein, NIH3T3 cells

  • 常规说明

    This product was changed from ascites to supernatant. Lot no’s high than GR240839-12 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 纯化说明

    Purified from tissue culture supernatant.

  • 克隆

    单克隆

  • 克隆编号

    5A6

  • 同种型

    IgG2b

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab119948于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA
1/10000.
ICC/IF
1/200 - 1/1000.
说明
WB
1/500 - 1/2000. Predicted molecular weight: 52 kDa.
ELISA
1/10000.
ICC/IF
1/200 - 1/1000.

靶标

  • 功能

    Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

  • 疾病相关

    Defects in FGG are a cause of thrombophilia.
    Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.

  • 序列相似性

    Contains 1 fibrinogen C-terminal domain.

  • 结构域

    A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

  • 翻译后修饰

    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Sulfation of C-terminal tyrosines increases affinity for thrombin.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • FGG antibody
      • FIBG_HUMAN antibody
      • Fibrinogen gamma chain antibody
      • Fibrinogen gamma polypeptide antibody
      • fibrinogen gamma-b chain antibody
      see all

    图片

    • Western blot - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Western blot - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Anti-Fibrinogen gamma chain antibody [5A6] (ab119948) at 1/500 dilution + Recombinant Fibrinogen gamma chain protein

      Predicted band size: 52 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Immunocytochemistry/ Immunofluorescence - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      Immunofluorescence analysis of NIH3T3 cells using ab119948 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
    • ELISA - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      ELISA - Anti-Fibrinogen gamma chain antibody [5A6] (ab119948)
      ELISA, using ab119948 at a dilution of 1/10000.

    文献 (9)

    发表研究结果有使用 ab119948？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab119948 被引用在 9 文献中.

    • Liu D  et al. GRSF1 antagonizes age-associated hypercoagulability via modulation of fibrinogen mRNA stability. Cell Death Dis 14:717 (2023). PubMed: 37923734
    • Gustafson KT  et al. cyc-DEP: Cyclic immunofluorescence profiling of particles collected using dielectrophoresis. Electrophoresis 43:1784-1798 (2022). PubMed: 35753078
    • Hao X  et al. The Role of Neutrophil Extracellular Traps in Early Microthrombosis and Brain Injury After Subarachnoid Hemorrhage in Mice. Transl Stroke Res N/A:1-14 (2022). PubMed: 35962915
    • Jiang C  et al. Fibrinogen promotes gallbladder cancer cell metastasis and extravasation by inducing ICAM1 expression. Med Oncol 40:10 (2022). PubMed: 36352295
    • Kuang M  et al. FGB and FGG derived from plasma exosomes as potential biomarkers to distinguish benign from malignant pulmonary nodules. Clin Exp Med 19:557-564 (2019). PubMed: 31576477
    • Wu L  et al. A proteomic analysis identifies candidate early biomarkers to predict ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients. Mol Med Rep 16:272-280 (2017). PubMed: 28534980
    • Benito-Jardón M  et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. Elife 6:N/A (2017). PubMed: 28092265
    • Schunke KJ  et al. A novel atherothrombotic model of ischemic stroke induced by injection of collagen into the cerebral vasculature. J Neurosci Methods 239:65-74 (2015). PubMed: 25314906
    • Liu T  et al. Immobilization of heparin/poly-l-lysine nanoparticles on dopamine-coated surface to create a heparin density gradient for selective direction of platelet and vascular cells behavior. Acta Biomater 10:1940-54 (2014). PubMed: 24342042

    客户评价及客户问答

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    Western blot abreview for Anti-Fibrinogen gamma chain antibody [5A6]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Loading amount
    20 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20%)
    Blocking step
    Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    提交于 Dec 06 2012

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