Anti-Fibrinogen alpha chain抗体[UC45] (ab19079)
![Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)](https://www.abcam.cn/ps/products/19/ab19079/Images/ab19079-28598-anti-fibrinogen-alpha-chain-antibody-uc45-flow-cytometry.jpg "Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)")
Key features and details
- Mouse monoclonal [UC45] to Fibrinogen alpha chain
- Suitable for: RIA, ICC, Flow Cyt, ELISA
- Reacts with: Mouse, Rat, Human
- Isotype: IgM
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Fibrinogen alpha chain抗体[UC45]
参阅全部 Fibrinogen Alpha Chain 一抗 -
描述
小鼠单克隆抗体[UC45] to Fibrinogen alpha chain -
宿主
Mouse -
特异性
This antibody reacts very strongly with the alpha chain of human fibrinogen, for further information please see Hogg 1983 paper quoted. -
经测试应用
适用于: RIA, ICC, Flow Cyt, ELISAmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Tissue, cells or virus corresponding to Human Fibrinogen alpha chain. Human acute monoblastic leukemia cells.
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常规说明
A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from tissue culture supernatant. -
Primary antibody说明
A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation. -
克隆
单克隆 -
克隆编号
UC45 -
骨髓瘤
P3-NS1/1-Ag4-1 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab19079于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| RIA |
Use at an assay dependent concentration.
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| ICC |
Use at an assay dependent concentration.
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| Flow Cyt |
Use 1µg for 106 cells.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
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| ELISA |
Use at an assay dependent concentration. PubMed: 24959311
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| 说明 |
|---|
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RIA
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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Flow Cyt
Use 1µg for 106 cells. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
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ELISA
Use at an assay dependent concentration. PubMed: 24959311 |
靶标
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功能
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
组织特异性
Plasma. -
疾病相关
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
序列相似性
Contains 1 fibrinogen C-terminal domain. -
结构域
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
翻译后修饰
The alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2243 Human
- Entrez Gene: 14161 Mouse
- Entrez Gene: 361969 Rat
- Omim: 134820 Human
- SwissProt: P02671 Human
- SwissProt: E9PV24 Mouse
- SwissProt: P06399 Rat
- Unigene: 351593 Human
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别名
- Ac1873 antibody
- Fba5e antibody
- FGA antibody
see all
图片
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Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)Overlay histogram showing THP1 cells stained with ab19079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19079, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab19079 被引用在 9 文献中.
- Lv LL et al. Effect of haptoglobin on the treatment of chronic hepatitis B with interferon. Exp Ther Med 18:1417-1425 (2019). PubMed: 31316628
- Matsuoka A et al. Novel strategy of ovarian cancer implantation: Pre-invasive growth of fibrin-anchored cells with neovascularization. Cancer Sci 110:2658-2666 (2019). PubMed: 31199029
- Yeung AM et al. Fibrin glue inhibits migration of ocular surface epithelial cells. Eye (Lond) 30:1389-1394 (2016). PubMed: 27367746
- Wu X et al. Peptide targeted high-resolution molecular imaging of prostate cancer with MRI. Am J Nucl Med Mol Imaging 4:525-36 (2014). PubMed: 25250202
- Richens JL et al. Practical detection of a definitive biomarker panel for Alzheimer's disease; comparisons between matched plasma and cerebrospinal fluid. Int J Mol Epidemiol Genet 5:53-70 (2014). ELISA ; Human . PubMed: 24959311
- Wu X et al. Molecular MRI of atherosclerotic plaque progression in an ApoE(-/-) mouse model with a CLT1 peptide targeted macrocyclic Gd(III) chelate. Am J Nucl Med Mol Imaging 3:446-55 (2013). PubMed: 24116353
- Linch DC et al. Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML. Blood 63:566-73 (1984). PubMed: 6365201
- Hogg N Human monocytes are associated with the formation of fibrin. J Exp Med 157:473-85 (1983). PubMed: 6822786
- Hogg N et al. Monoclonal antibody with specificity for monocytes and neurons. Cell 24:875-84 (1981). PubMed: 6788378