Anti-Fibrillarin抗体[38F3] - Nucleolar Marker

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (AB4566)

ICC analysis of HeLa cells stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to vimentin (red) and counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with DAPI(blue). The vimentin antibody was used at a dilution of 1/1000 and the fibrillarin monoclonal at 1/100. Cultures were processed using standard fixation and staining procedure (in protocol section).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (AB4566)

Human neuroblastoma line SH-SY5Y stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red) and counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using standard fixation and staining procedure (in protocol section).

• ICC/IF

AbReview14675****

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (AB4566)

ab staining Fibrillarin in Human melanoma A7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in 1% BSA) for 24 hours at 4°C. A FITC-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.

Image is courtesy of Cesar Camacho

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (AB4566)

High magnification view of human Hek293 cell nuclei stained with mouse monoclonal to fibrillarin (green), counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixation and staining procedure (in protocol section).

• Flow Cyt

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Flow Cytometry - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (AB4566)

Overlay histogram showing HEK293 cells stained with ab4566 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4566, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (AB4566)

Rat neurons and glial stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red). Cells were counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixation and staining procedure (in protocol section).

• WB

AbReview27713****

Western blot - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (AB4566)

Mouse embryonic fibroblast fractionation.

Cytopl - cytoplasmic fraction.
Nucl - nuclear fraction.
20 µg of each loaded.

ab4566 used at a 1/2000 dilution.
The secondary used was an Alexa-Fluor 680 conjugated goat anti-mouse polyclonal used at a 1/10000 dilution.

All lanes:

Western blot - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566)

Predicted band size: 33 kDa

false

Image courtesy of an anonymous Abreview.

• WB

AbReview19782****

Western blot - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (AB4566)

All lanes:

Western blot - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566) at 1/2000 dilution

Lane 1:

cytoplasmic protein fraction of HeLa cells at 20 µg with LI-COR® Odyssey® Blocking Buffer, 45 minutes at room temperature at 50 %

Lane 2:

nuclear protein fraction of HeLa cells at 20 µg with LI-COR® Odyssey® Blocking Buffer, 45 minutes at room temperature

Secondary

All lanes:

AlexaFluor 680 goat anti-mouse at 1/10000 dilution

Predicted band size: 33 kDa

Observed band size: 34 kDa

false

Exposure time: 1s

Image is courtesy of Dr Svetlana Khoronenkova

• WB

Supplier Data

Western blot - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (AB4566)

All lanes:

Western blot - Anti-Fibrillarin antibody [38F3] - Nucleolar Marker (ab4566) at 1/500 dilution

Lane 2:

C6 cytosol fraction

Lane 3:

C6 nuclear fraction

Lane 4:

HEK-293 cytosol fraction

Lane 5:

HEK-293 nuclear fraction

Lane 6:

NIH-3T3 cytosol fraction

Lane 7:

NIH-3T3 nuclear fraction

Predicted band size: 33 kDa

Observed band size: 37 kDa

false