Anti-FHOD1抗体(ab73443)
Key features and details
- Mouse polyclonal to FHOD1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FHOD1抗体
参阅全部 FHOD1 一抗 -
描述
小鼠多克隆抗体to FHOD1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human FHOD1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- K-562 cell lysate and FHOD1 transfected 293T cell lysate human spleen
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73443于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 127 kDa.
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| IHC-P |
Use a concentration of 13 µg/ml.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 127 kDa. |
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IHC-P
Use a concentration of 13 µg/ml. |
靶标
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功能
Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. -
组织特异性
Ubiquitous. Highly expressed in spleen. -
序列相似性
Belongs to the formin homology family.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. -
结构域
Regulated by intramolecular binding to a C-terminal auto-inhibitory domain. Effector binding abolishes this interaction and activates the protein. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Predominantly cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 29109 Human
- Omim: 606881 Human
- SwissProt: Q9Y613 Human
- Unigene: 95231 Human
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别名
- FH1/FH2 domain containing protein antibody
- FH1/FH2 domain containing protein 1 antibody
- FH1/FH2 domain-containing protein 1 antibody
see all
图片
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Western blot - Anti-FHOD1 antibody (ab73443)All lanes : Anti-FHOD1 antibody (ab73443) at 1/500 dilution
Lane 1 : FHOD1 transfected 293T cell lysate
Lane 2 : non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : goat anti-mouse IgG(H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 127 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-FHOD1 antibody (ab73443)Anti-FHOD1 antibody (ab73443) at 1/500 dilution + K-562 cell lysate at 25 µg
Secondary
goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 127 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHOD1 antibody (ab73443)Immunoperoxidase staining of formalin-fixed paraffin-embedded human spleen using ab73443, at 3 ug/ml of purified version of the antibody.
数据表及文件
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Datasheet download
文献 (7)
ab73443 被引用在 7 文献中.
- Jiang C et al. FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells. Oncol Lett 22:712 (2021). PubMed: 34457067
- Zuidscherwoude M et al. Loss of mDia1 and Fhod1 impacts platelet formation but not platelet function. Platelets N/A:1-12 (2020). PubMed: 32981398
- Shi X et al. Active FHOD1 promotes the formation of functional actin stress fibers. Biochem J 476:2953-2963 (2019). PubMed: 31657439
- Schwartz C et al. Lamins and nesprin-1 mediate inside-out mechanical coupling in muscle cell precursors through FHOD1. Sci Rep 7:1253 (2017). WB . PubMed: 28455503
- Dwyer J et al. The formin FHOD1 in cardiomyocytes. Anat Rec (Hoboken) 297:1560-70 (2014). PubMed: 25125170
- Viswanatha R et al. Interactome analysis reveals ezrin can adopt multiple conformational states. J Biol Chem 288:35437-51 (2013). WB ; Human . PubMed: 24151071
- Ogembo JG et al. SIRPa/CD172a and FHOD1 are unique markers of littoral cells, a recently evolved major cell population of red pulp of human spleen. J Immunol 188:4496-505 (2012). IHC-P ; Human . PubMed: 22490440
