Anti-FH/Fumarase抗体(ab113963)
Key features and details
- Goat polyclonal to FH/Fumarase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-FH/Fumarase抗体
参阅全部 FH/Fumarase 一抗 -
描述
山羊多克隆抗体to FH/Fumarase -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Zebrafish, Chinese hamster
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免疫原
Synthetic peptide corresponding to Human FH/Fumarase aa 150-250 (internal sequence) (Cysteine residue).
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阳性对照
- Human Heart and Human Skeletal Muscle tissue Human Kidney lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113963于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.01 µg/ml. Predicted molecular weight: 55 kDa.
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| IHC-P |
Use a concentration of 3.75 µg/ml.
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| 说明 |
|---|
|
WB
Use a concentration of 0.01 µg/ml. Predicted molecular weight: 55 kDa. |
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IHC-P
Use a concentration of 3.75 µg/ml. |
靶标
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功能
Also acts as a tumor suppressor. -
通路
Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. -
疾病相关
Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839]. -
序列相似性
Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. -
细胞定位
Cytoplasm and Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 520260 Cow
- Entrez Gene: 480092 Dog
- Entrez Gene: 2271 Human
- Entrez Gene: 14194 Mouse
- Entrez Gene: 100627128 Pig
- Entrez Gene: 24368 Rat
- Entrez Gene: 393938 Zebrafish
- Omim: 136850 Human
see all -
别名
- FH antibody
- Fumarase antibody
- Fumarate hydratase antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (ab113963)Immunohistochemical staining of Human Heart using ab113963 at 3.75µg/ml
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (ab113963)Immunohistochemical staining of Human Skeletal Muscle using ab113963 at 3.75µg/ml -
Western blot - Anti-FH/Fumarase antibody (ab113963)Anti-FH/Fumarase antibody (ab113963) at 0.01 µg/ml + Human Kidney lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Primary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab113963 被引用在 3 文献中.
- Zecchini V et al. Fumarate induces vesicular release of mtDNA to drive innate immunity. Nature 615:499-506 (2023). PubMed: 36890229
- Jiang Y et al. Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation. Nat Cell Biol 17:1158-68 (2015). WB ; Human . PubMed: 26237645
- Zecchini V et al. Nuclear ARRB1 induces pseudohypoxia and cellular metabolism reprogramming in prostate cancer. EMBO J 33:1365-82 (2014). WB, ICC/IF . PubMed: 24837709