Anti-FGFR1抗体[M5G10] (ab824)

Reviews (1)Q&A (14)References (17)
Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody [M5G10] (ab824)
  • Flow Cytometry - Anti-FGFR1 antibody [M5G10] (ab824)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody [M5G10] (ab824)

Key features and details

  • Mouse monoclonal [M5G10] to FGFR1
  • Suitable for: ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-FGFR1抗体[M5G10]
    参阅全部 FGFR1 一抗

  • 描述

    小鼠单克隆抗体[M5G10] to FGFR1

  • 宿主

    Mouse

  • 特异性

    Reacts with both alpha and beta isoforms.

  • 经测试应用

    适用于: ICC/IF, IHC-P, Flow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment corresponding to Human FGFR1. Recombinant human ectodomain of FGFr1 beta expressed in E. coli beginning with proline 23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

  • 表位

    Epitope is within the sequence His 241 and Val 267 between Ig loops II and III. Epitope is masked in undenatured FGFr1a.

  • 阳性对照

    • This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: SKNSH.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab824于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
说明
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Receptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL (By similarity). A shorter form of the receptor could be a receptor for FGF1 (aFGF).

  • 组织特异性

    Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.

  • 疾病相关

    Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
    Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
    Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
    Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
    Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • 翻译后修饰

    Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.

  • 细胞定位

    Membrane. Nucleus. Cytoplasm. Cytoplasmic vesicle
  • Information by UniProt

  • 数据库链接

    • 别名

      • Basic fibroblast growth factor receptor 1 antibody
      • bFGF-R-1 antibody
      • BFGFR antibody
      • CD331 antibody
      • CEK antibody
      • FGFBR antibody
      • FGFR 1 antibody
      • FGFR-1 antibody
      • FGFR1 antibody
      • FGFR1/PLAG1 fusion antibody
      • FGFR1_HUMAN antibody
      • fibroblast growth factor receptor 1 antibody
      • FLG antibody
      • FLT-2 antibody
      • FLT2 antibody
      • Fms-like gene antibody
      • Fms-like tyrosine kinase 2 antibody
      • fms-related tyrosine kinase 2 antibody
      • HBGFR antibody
      • heparin-binding growth factor receptor antibody
      • HH2 antibody
      • HRTFDS antibody
      • hydroxyaryl-protein kinase antibody
      • KAL2 antibody
      • N-SAM antibody
      • OGD antibody
      • Proto-oncogene c-Fgr antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody [M5G10] (ab824)
      Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 antibody [M5G10] (ab824)

      ICC/IF image of ab824 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab824 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      This image was generated using the ascites version of the product. 

    • Flow Cytometry - Anti-FGFR1 antibody [M5G10] (ab824)
      Flow Cytometry - Anti-FGFR1 antibody [M5G10] (ab824)

      Overlay histogram showing MCF7 cells stained with ab824 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab824, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

      This image was generated using the ascites version of the product. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody [M5G10] (ab824)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 antibody [M5G10] (ab824)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal salivary gland labelling FGFR1 with ab824 at 10µg/ml.

      This image was generated using the ascites version of the product. 

    文献 (17)

    发表研究结果有使用 ab824？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab824 被引用在 17 文献中.

    • Cimmino F  et al. FGFR1 is a potential therapeutic target in neuroblastoma. Cancer Cell Int 22:174 (2022). PubMed: 35488346
    • Zhou DW  et al. Force-FAK signaling coupling at individual focal adhesions coordinates mechanosensing and microtissue repair. Nat Commun 12:2359 (2021). PubMed: 33883558
    • Liu H  et al. A novel multikinase inhibitor SKLB-YTH-60 ameliorates inflammation and fibrosis in bleomycin-induced lung fibrosis mouse models. Cell Prolif 54:e13081 (2021). PubMed: 34121240
    • Sheng L  et al. Atractylenolide III predisposes miR-195-5p/FGFR1 signaling axis to exert tumor-suppressive functions in liver cancer. J Food Biochem 45:e13582 (2021). PubMed: 33768570
    • Tang J  et al. microRNA-199a counteracts glucocorticoid inhibition of bone marrow mesenchymal stem cell osteogenic differentiation through regulation of Klotho expression in vitro. Cell Biol Int 44:2532-2540 (2020). PubMed: 32869899
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 15 Abreviews or Q&A

    Western blot abreview for Anti-FGFR1 antibody [M5G10]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cancer cell lines)
    Loading amount
    15 µg
    Specification
    Cancer cell lines
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Valentina André

    Verified customer

    提交于 Sep 28 2012

    Question

    Customer kindly contacted us regarding FC graph on ds (lists ab79976), customer has not been seeing the same peak shift as indicated in image. WB is turning up negative as well, but the antibody is working in ELISA. The epitope is listed as being masked in undenatured FGFr1a, customer checking that this is correctly stated.

    Read More
    Answer

    Thank you for contacting Abcam.

    Thank you for pointing out the error in figure legends for ab824 and ab79976. The flow cytometry figure legends for the two antibodies have been switched on the online datasheet to correspond with the correct antibody.
    ab824: (https://www.abcam.com/fgfr1-antibody-m5g10-ab824.html)

    ab824 is specifically raised against the ectodomain FGFR1 beta isoform. Thus, the statement in the epitope description that the "epitope is masked in undenatured FGFr1a" refers to the specificity of this antibody for the FGFR1 beta isoform.

    Below are some general troubleshooting tips for flow cytometry, if you are experiencing issues with ab824 please reply with further sample, protocol and troubleshooting steps that have been taken. These will not only help me to fully understand the problem but are essential should further testing of this product be needed as well.
    https://www.abcam.com/index.html?pageconfig=resource&rid=11443

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Question

    AbID: 824, Anti-FGFR1 antibody [M5G10] Rating: Inconclusive Image: Anti-FGFR1 antibody [M5G10] for Western blot (Human) Sample: Species: Human Type: Cell lysate - whole cell Loading amount: 15 µg Specification: Cancer cell lines Application: Application: Gel Running Conditions: Reduced Denaturing Blocking step: Milk as blocking agent for 30 mins at RT°C Blocking Concentration: 5% Other detail: Dilution: 1/200 Incubation time: 18 hours at 4°C Diluent: TBS-T 5% milk Secondary Antibody: Name: Non-Abcam Antibody was used: goat anti-mouse IgG-HRP Conjugation: HRP Dilution: 1/5000 Detection: Detection method: ECL Exposure: 30 minutes and 0 seconds Positive Control: DU145 (human prostate cancer cell lines), MCF7 (human breast adenocarcinoma cell line) and GN11 (murine cell line of immortalized gonadotropin-releasing hormone-expressing neurons) stably transfected with the human FGFR1 gene Negative Control: GN11 non transfected and GN11 stably transfected with the empty vector Additional Data: Additional Notes: Lane 1: GN11 cell lysates (non transfected) Lane 2: GN11 cell lysates stably transfected with the empty vector (mock) Lane 3: GN11 cell lysates stably transfected with the human FGFR1 gene Lane 4: DU145 cell lysate Lane 5: MCF7 cell lysate

    Read More
    Answer

    Thanks for submitting your Abreview.

    As yourated your experience with this antibody as 'Inconclusive' I just wanted to let you know that if you feel the antibody is not working as expected I can offer a replacement of any primary antibody, credit or refund. Alternatively, I'm happy to help you troubleshoot this issue as well.

    Please let me know if you have any questions. If you would like a replacement, please pass along the new product number you are interested in as well as the PO or Abcan order reference number associated with this purchase of ab824.

    Thanks very much. I look forward to hearing from you.

    Read More

    Question

    I am writing in regards to order# 1044955.We just receivedab7192 to replace ab824 butwe actuallywanted AB71928. Due tomy error in listing the incorrect cat#, we got something that we don't need. Would it be possible to exchage AB7192 for AB71928? I apologize for all of the inconvenience this may cause...

    Read More
    Answer

    Thanks for your email.
    Earlier today I sent you and email containing instructions on returning an antibody to Abcam. Below, please find those same instructions:
    ===========
    Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:
    - Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
    - Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet)
    - Please return via an overnight courier such as UPS, DHL, or FEDEX.
    - Ship to the following address:
    Attn: Returns ORDER REF: (_____)
    Abcam Inc
    One Kendall Square
    Bldg 200, Suite B2304
    Cambridge, MA 02139
    Please be advised that a 20% restock fee will apply to this order. The most up-to-date information regarding your order, invoice, and credit note status is available online in your Abcam account information. If your order was placed by credit card, your refund will be issued to your credit card. If your order was placed by Purchase Order, all customers must submit a credit note with their invoice to their Accounts Payable department to be handled. The Accounts Payable must have a copy of the credit note so the original invoice can be updated to reflect the correct amount owed.
    If you have any other questions please do not hesitate to contact us!
    Email: US.orders@Abcam.com
    Phone: 888-77-ABCAM(22226)
    Fax: 866 739 9884
    Customer Service hours of operation:
    Monday-Friday 8:30 AM- 9:00PM EST

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    Question

    Dear Abcam,
    Thank you for replacing these items. Unfortunately, I made a mistake in the replacement request. For AB824, I wanted AB71928 (Anti-FGFR1 antibody) not AB7192. (I left the 8 out at the end)
    It is my fault. I have a question. Would it possible for us to return AB7192 and get AB71928? If we cannot return AB7192, can you please send AB71928 and we will pay for AB7192.
    You can also ship AB71928 with the backordered item. Thanks!

    Read More
    Answer

    Thank you for contacting Abcam. Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:
    - Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
    - Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet)
    - Please return via an overnight courier such as UPS, DHL, or FEDEX.
    - Ship to the following address:
    Attn: Returns ORDER REF: (_____)
    Abcam Inc
    One Kendall Square
    Bldg 200, Suite B2304
    Cambridge, MA 02139
    Please be advised that a 20% restock fee will apply to this order. The most up-to-date information regarding your order, invoice, and credit note status is available online in your Abcam account information. If your order was placed by credit card, your refund will be issued to your credit card. If your order was placed by Purchase Order, all customers must submit a credit note with their invoice to their Accounts Payable department to be handled. The Accounts Payable must have a copy of the credit note so the original invoice can be updated to reflect the correct amount owed.
    If you have any other questions please do not hesitate to contact us!
    Email: US.orders@Abcam.com
    Phone: 888-77-ABCAM(22226)
    Fax: 866 739 9884
    Customer Service hours of operation:
    Monday-Friday 8:30 AM- 9:00PM EST

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    Question

    2) For AB824 --> ab7192
    For AB10648 -->ab119237

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    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements on the following order numbers:
    You will receive ab7192 to replace ab824
    . You should receive this on Friday March 2.
    You will receive ab119237 to replace ab10648.
    You should receive this on or around Tuesday March 7 (it is back-ordered).
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
    I wish you the best of luck with your research.

    Read More

    Question

    Yes, your assessment was correct. It was only ab10648 and ab824 that were not detecting protein in the expected subcellular region. We just need replacements for these two items. Thanks!

    Read More
    Answer

    Thanks for your reply.
    I'll just need to locate your order information for ab10648 and ab824 on our system so I can process the replacement order.
    If you could provide me with:
    1) The PO number or Abcam order reference number associated with this order.
    2) The catalog numbers of the two antibodies you would like as replacements.
    Thanks!

    Read More

    Question

    Dear Abcam,
    Thank you for your response. We have not tried staining these tissue sections with other antibodies... We would like try it again with these three abcamantibodies. Can you please send us replacements for these antibodies that didn't work as expected?

    Read More
    Answer

    I would be happy to send along replacement antibodies to replace those not working as expected.
    Based on your last reply, it seemed that ab10648 and ab824 were not detecting protein in the expected subcellular region. The report in your email also noted ab65973 was detecting protein in the cytoplasm and nucleus which is expected.
    Is this assessment correct?

    Read More

    Question

    Dear Abcam,
    Thanks for your reply. We have some concerns about the performance of these products. Please see attached document from one of our scientists. Can you please explain these results? Thanks!

    Read More
    Answer

    Thank you for the information.
    You are correct that ab10648 and ab824 should be staining the cell surface. ab65973, which stains FGF basic, is expected to be nuclear and/or cytoplasmic.
    Have you stained these same tissue sections successfully with any other antibodies? It is quite odd that 3 different antibodies would not be working as expected.

    Read More

    Question

    I have a question about three of your antibody products. 1)Anti-FGFR2 antibody (ab10648), 2)Anti-FGFR1 antibody [M5G10] (ab824), 3)Anti-FGF basic antibody - Aminoterminal end (ab65973))
    Where is the expression location? Is the positive result located in the membrane or the nucleus?

    Read More
    Answer

    Thank you for your enquiry.
    Anti-FGFR2 antibody (ab10648) = cell-surface receptor for fibroblast growth factors
    Anti-FGFR1 antibody [M5G10] (ab824) = cell-surface receptor for fibroblast growth factors
    Anti-FGF basic antibody - Aminoterminal end (ab65973) = can be nuclear or cytoplasmic, depending on isoform
    I hope this is helpful. Please contact us again if you have any further questions.

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    1-10 of 15 Abreviews or Q&A

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