Anti-FGFR1抗体[M5G10] (ab824)

Thank you for contacting Abcam.

Thank you for pointing out the error in figure legends for ab824 and ab79976. The flow cytometry figure legends for the two antibodies have been switched on the online datasheet to correspond with the correct antibody.
ab824: (https://www.abcam.com/fgfr1-antibody-m5g10-ab824.html)

ab824 is specifically raised against the ectodomain FGFR1 beta isoform. Thus, the statement in the epitope description that the "epitope is masked in undenatured FGFr1a" refers to the specificity of this antibody for the FGFR1 beta isoform.

Below are some general troubleshooting tips for flow cytometry, if you are experiencing issues with ab824 please reply with further sample, protocol and troubleshooting steps that have been taken. These will not only help me to fully understand the problem but are essential should further testing of this product be needed as well.
https://www.abcam.com/index.html?pageconfig=resource&rid=11443

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Thanks for submitting your Abreview.

As yourated your experience with this antibody as 'Inconclusive' I just wanted to let you know that if you feel the antibody is not working as expected I can offer a replacement of any primary antibody, credit or refund. Alternatively, I'm happy to help you troubleshoot this issue as well.

Please let me know if you have any questions. If you would like a replacement, please pass along the new product number you are interested in as well as the PO or Abcan order reference number associated with this purchase of ab824.

Thanks very much. I look forward to hearing from you.

Thanks for your email.
Earlier today I sent you and email containing instructions on returning an antibody to Abcam. Below, please find those same instructions:
===========
Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:
- Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
- Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet)
- Please return via an overnight courier such as UPS, DHL, or FEDEX.
- Ship to the following address:
Attn: Returns ORDER REF: (_____)
Abcam Inc
One Kendall Square
Bldg 200, Suite B2304
Cambridge, MA 02139
Please be advised that a 20% restock fee will apply to this order. The most up-to-date information regarding your order, invoice, and credit note status is available online in your Abcam account information. If your order was placed by credit card, your refund will be issued to your credit card. If your order was placed by Purchase Order, all customers must submit a credit note with their invoice to their Accounts Payable department to be handled. The Accounts Payable must have a copy of the credit note so the original invoice can be updated to reflect the correct amount owed.
If you have any other questions please do not hesitate to contact us!
Email: US.orders@Abcam.com
Phone: 888-77-ABCAM(22226)
Fax: 866 739 9884
Customer Service hours of operation:
Monday-Friday 8:30 AM- 9:00PM EST

Thank you for contacting Abcam. Included in this email are the instructions for returning your item/s to Abcam. When you return the goods, please ensure that you follow the procedures as below:
- Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today.
- Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet)
- Please return via an overnight courier such as UPS, DHL, or FEDEX.
- Ship to the following address:
Attn: Returns ORDER REF: (_____)
Abcam Inc
One Kendall Square
Bldg 200, Suite B2304
Cambridge, MA 02139
Please be advised that a 20% restock fee will apply to this order. The most up-to-date information regarding your order, invoice, and credit note status is available online in your Abcam account information. If your order was placed by credit card, your refund will be issued to your credit card. If your order was placed by Purchase Order, all customers must submit a credit note with their invoice to their Accounts Payable department to be handled. The Accounts Payable must have a copy of the credit note so the original invoice can be updated to reflect the correct amount owed.
If you have any other questions please do not hesitate to contact us!
Email: US.orders@Abcam.com
Phone: 888-77-ABCAM(22226)
Fax: 866 739 9884
Customer Service hours of operation:
Monday-Friday 8:30 AM- 9:00PM EST

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements on the following order numbers:
You will receive ab7192 to replace ab824
. You should receive this on Friday March 2.
You will receive ab119237 to replace ab10648.
You should receive this on or around Tuesday March 7 (it is back-ordered).
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

Thanks for your reply.
I'll just need to locate your order information for ab10648 and ab824 on our system so I can process the replacement order.
If you could provide me with:
1) The PO number or Abcam order reference number associated with this order.
2) The catalog numbers of the two antibodies you would like as replacements.
Thanks!

I would be happy to send along replacement antibodies to replace those not working as expected.
Based on your last reply, it seemed that ab10648 and ab824 were not detecting protein in the expected subcellular region. The report in your email also noted ab65973 was detecting protein in the cytoplasm and nucleus which is expected.
Is this assessment correct?

Thank you for the information.
You are correct that ab10648 and ab824 should be staining the cell surface. ab65973, which stains FGF basic, is expected to be nuclear and/or cytoplasmic.
Have you stained these same tissue sections successfully with any other antibodies? It is quite odd that 3 different antibodies would not be working as expected.

Thank you for your enquiry.
Anti-FGFR2 antibody (ab10648) = cell-surface receptor for fibroblast growth factors
Anti-FGFR1 antibody [M5G10] (ab824) = cell-surface receptor for fibroblast growth factors
Anti-FGF basic antibody - Aminoterminal end (ab65973) = can be nuclear or cytoplasmic, depending on isoform
I hope this is helpful. Please contact us again if you have any further questions.