Anti-FGFR1 alpha抗体[M2F12] (ab829)

No - this antibody is specific for FGFR1.

Ab829 binds on the cell surface.

Thank you for your patience. I have been discussing this complaint with my colleagues. Unfortunately, we do not really know why this antibody did not perform well for you. We haven't had any complaints about this one. In fact, most of our customers for this antibody use it for immunohistochemistry. A couple of things stand out in your protocol: (1) You pretreat the tissue sections with bovine serum albumin to block non-specific binding. Because BSA doesn't contain any immunoglobulin that can occupy Fc receptors that might be present on cells in tissues, we always recommend blocking tissue sections with an unrelated normal serum, such as goat or rabbit. We realize, though, that you feel that blocking with BSA worked well for the Santa Cruz antibody, so you did probably not inclined to try blocking with normal serum. (2) It was difficult to compare the photos of the tissue staining that you provided because each photo appeared to be a different tissue section than the other photos. Where your felt that you saw very high background staining (with Abcam antibody at 1:100), we think you used too high a concentration of primary antibody. According to our records, the batch that you purchased was at a concentration of 1.65 mg/ml. Therefore, a 1:100 dilution of this would give a working solution of 16.5 ug/ml. This is a rather high antibody concentration for IHC. Most of our antibodies work in the range of 3-5 ug/ml. Of course, the 1:1000 dilution gives a working solution of 1.65 ug/ml, and this might be too low. We could send you a replacement vial, but it would be the same lot as what you already received (it is the only lot we have at this time), and we would guess that you wouldn't be eager to try this one again. Perhaps the most expedient solution is for you a credit note or refund. Please do let me know what you would like to do.