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Cardiovascular Angiogenesis Growth Factors FGF FGF Receptors

Anti-FGFR1 alpha抗体[M2F12] (ab829)

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Reviews (2)Q&A (3)References (12)

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Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 alpha antibody [M2F12] (ab829)

    Key features and details

    • Mouse monoclonal [M2F12] to FGFR1 alpha
    • Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, IP
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG2a

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    概述

    • 产品名称

      Anti-FGFR1 alpha抗体[M2F12]
      参阅全部 FGFR1 alpha 一抗
    • 描述

      小鼠单克隆抗体[M2F12] to FGFR1 alpha
    • 宿主

      Mouse
    • 特异性

      Reacts with alpha isoform of FRFR1.
    • 经测试应用

      适用于: IHC-P, WB, IHC-Fr, ICC/IF, IPmore details
    • 种属反应性

      与反应: Mouse, Rat, Human
    • 免疫原

      Recombinant fragment corresponding to Human FGFR1 alpha. Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

    • 表位

      NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a.
    • 阳性对照

      • IHC: Rat prostate tissue.
    • 常规说明

      This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.40
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein G purified
    • 纯化说明

      Purified from TCS
    • 克隆

      单克隆
    • 克隆编号

      M2F12
    • 同种型

      IgG2a
    • 研究领域

      • Cardiovascular
      • Angiogenesis
      • Growth Factors
      • FGF
      • FGF Receptors
      • Signal Transduction
      • Growth Factors/Hormones
      • FGF
      • Cancer
      • Growth factors
      • FGF
      • Neuroscience
      • Development

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant human FGFR1 alpha protein (Fc Chimera) (ab55758)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab829于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    Use at an assay dependent concentration. PubMed: 19728793
    WB (1)
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    Use a concentration of 10 µg/ml.
    IP
    Use at an assay dependent concentration.
    说明
    IHC-P
    Use at an assay dependent concentration. PubMed: 19728793
    WB
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    Use a concentration of 10 µg/ml.
    IP
    Use at an assay dependent concentration.

    靶标

    • 相关性

      FGFR1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family and contains an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGFR1 is widely expressed, with distinct isoforms expressed in specific tissues. FGFR1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGFR1 result in Pfeiffer syndrome associated with craniosynostosis. FGFR1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGFR1 has been shown to interact with N-cadherin and NCAM.
    • 细胞定位

      Cell Membrane
    • 数据库链接

      • Entrez Gene: 2260 Human
      • Entrez Gene: 14182 Mouse
      • Entrez Gene: 79114 Rat
      • Omim: 136350 Human
      • SwissProt: P11362 Human
      • SwissProt: P16092 Mouse
      • SwissProt: Q04589 Rat
      • 别名

        • BFGFR antibody
        • CD331 antibody
        • CEK antibody
        • FGFBR antibody
        • FGFR1 antibody
        • FLG antibody
        • FLT2 antibody
        • FMS like tyrosine kinase 2 antibody
        • HBGFR antibody
        • KAL2 antibody
        • N SAM antibody
        • OGD antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 alpha antibody [M2F12] (ab829)
        Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 alpha antibody [M2F12] (ab829)

        ICC/IF image of ab829 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab829, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

        This image was generated using the ascites version of the product. 

      实验方案

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (12)

      发表研究结果有使用 ab829?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab829 被引用在 12 文献中.

      • Formisano L  et al. Aberrant FGFR signaling mediates resistance to CDK4/6 inhibitors in ER+ breast cancer. Nat Commun 10:1373 (2019). PubMed: 30914635
      • Zhang GY  et al. Thyroxine (T4) may promote re-epithelialisation and angiogenesis in wounded human skin ex vivo. PLoS One 14:e0212659 (2019). PubMed: 30925152
      • Voytyuk I  et al. BACE2 distribution in major brain cell types and identification of novel substrates. Life Sci Alliance 1:e201800026 (2018). PubMed: 30456346
      • Mao Y  et al. VEGF-A/VEGFR-2 and FGF-2/FGFR-1 but not PDGF-BB/PDGFR-ß play important roles in promoting immature and inflammatory intraplaque angiogenesis. PLoS One 13:e0201395 (2018). PubMed: 30125282
      • Nakamichi M  et al. Basic Fibroblast Growth Factor Induces Angiogenic Properties of Fibrocytes to Stimulate Vascular Formation during Wound Healing. Am J Pathol 186:3203-3216 (2016). PubMed: 27773739
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-5 of 5 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FGFR1 alpha antibody [M2F12]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Formalin Fixed Paraffin Embedded)
      Permeabilization
      No
      Specification
      Formalin Fixed Paraffin Embedded
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Nov 12 2018

      Western blot abreview for Anti-FGFR1 alpha antibody [M2F12]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Neuronal cells)
      Gel Running Conditions
      Non-reduced Denaturing (4-12%)
      Loading amount
      25 µg
      Specification
      Neuronal cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Dec 19 2016

      Question

      Customer would like to know if this antibody binds to FGFR4 as well as FGFR1?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 23 2004

      Answer

      No - this antibody is specific for FGFR1.

      Read More

      Abcam Scientific Support

      回复于 Jun 25 2004

      Question

      Concerning the epitope - does ab829 bind on the cell surface or on the inside?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 29 2004

      Answer

      Ab829 binds on the cell surface.

      Read More

      Abcam Scientific Support

      回复于 Mar 30 2004

      Question

      I will try to answer all your questions below, and I also enclose pictures of staining results of frozen sections. Hope to hear from you. 1. Please describe the problem (high background, no staining etc).High background and weak signal from the antibody 2. On what material are you testing the antibody in IHC? Human pancreas tumors, grwon in SCID mice. ï Species? Human and maybe mouse (ingrowth of mouce cells into the tumor) ï Cell line? ï Tissue? Tumors 3. How did you fix the samples? Acetone Ethanol, methanol Acetone: Acetone Paraformaldehyde Other 4. Did you apply antigen retrieval step? No >ï Enzymatic method >ï Heat mediated technique >ï Other 5. How did you block the unspecific binding sites? 3% bovine serum albumine 6. Primary antibody Species: Human, mouse, rat Dilutions: 1:100, 1:1000 Incubation time: over night at 4 degrees. Washes: 3x5 minutes ï Specification (in which species was it raised against)? ï At what dilution(s) have you tested this antibody? ï Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 7. Secondary antibody ï What secondary antibody are you using? ï Specification (in which species was it raised against)? ï At what dilution(s) have you tested this antibody? ï Incubation, wash steps? ï Do you know whether the problems you are experiencing come from the >secondary? ï What detection method are you using? Secondary: Alexa goat anti-mouse, HCA (highly cross adsorbed); Molecular Probes. Dilutions: 1:1000 Incubation: 1 hour room temp. Wash: 3x10 min I daubt that the problems are due to the secondary as I use these type of secondary antibodies every week. Detection: immunoflourescence 8. Background staining ï Please provide an image of your staining It is included. 9. Which detection system did you use? Belive I have answered this question in panel 7. 10. Did you apply positive and negative controls along with the samples? Please specify. I always try primary alone, and secondary alone. 11. Optimization attempts ï How many times have you tried the IHC? ï Do you obtain the same results every time? ï What steps have you altered? Times with this antibody: 2 times Yes, the same result but at two different concentrations, which means weaker signal/background with a more diluted primary antibody. I have altered the dilutions. My positive control is the double staining of CD31 in both cases. I also include a picture of FGFR-1 and CD31 staining of FGFR-1 from Santa Cruz. Finally, we would like to reassure you that Abcam policy dictates that if a product does not performed as stated on the datasheet; we will offer a full reimbursement or provide a replacement of equivalent value. We are looking forward to hearing from you soon.

      Read More

      Abcam community

      Verified customer

      Asked on Mar 01 2004

      Answer

      Thank you for your patience. I have been discussing this complaint with my colleagues. Unfortunately, we do not really know why this antibody did not perform well for you. We haven't had any complaints about this one. In fact, most of our customers for this antibody use it for immunohistochemistry. A couple of things stand out in your protocol: (1) You pretreat the tissue sections with bovine serum albumin to block non-specific binding. Because BSA doesn't contain any immunoglobulin that can occupy Fc receptors that might be present on cells in tissues, we always recommend blocking tissue sections with an unrelated normal serum, such as goat or rabbit. We realize, though, that you feel that blocking with BSA worked well for the Santa Cruz antibody, so you did probably not inclined to try blocking with normal serum. (2) It was difficult to compare the photos of the tissue staining that you provided because each photo appeared to be a different tissue section than the other photos. Where your felt that you saw very high background staining (with Abcam antibody at 1:100), we think you used too high a concentration of primary antibody. According to our records, the batch that you purchased was at a concentration of 1.65 mg/ml. Therefore, a 1:100 dilution of this would give a working solution of 16.5 ug/ml. This is a rather high antibody concentration for IHC. Most of our antibodies work in the range of 3-5 ug/ml. Of course, the 1:1000 dilution gives a working solution of 1.65 ug/ml, and this might be too low. We could send you a replacement vial, but it would be the same lot as what you already received (it is the only lot we have at this time), and we would guess that you wouldn't be eager to try this one again. Perhaps the most expedient solution is for you a credit note or refund. Please do let me know what you would like to do.

      Read More

      Abcam Scientific Support

      回复于 Mar 11 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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