重组Anti-FGF9/GAF抗体[EPR19937] - BSA and Azide free (ab223543)
![Western blot - Anti-FGF9/GAF antibody [EPR19937] - BSA and Azide free (ab223543)](https://www.abcam.cn/ps/products/223/ab223543/Images/ab223543-282797-ab206408WB.jpg "Western blot - Anti-FGF9/GAF antibody [EPR19937] - BSA and Azide free (ab223543)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19937] to FGF9/GAF - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-FGF9/GAF抗体[EPR19937] - BSA and Azide free
参阅全部 FGF9/GAF 一抗 -
描述
兔单克隆抗体[EPR19937] to FGF9/GAF - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human FGF9/GAF active protein; human fetal brain, fetal kidney and ovary cancer lysates; mouse kidney and brain lysates; rat brain lysate; C6, PC-12, NIH/3T3 and U-87 MG whole cell lysates.
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常规说明
ab223543 is the carrier-free version of ab206408.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19937 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223543于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). |
靶标
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功能
May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. -
组织特异性
Glial cells. -
疾病相关
Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961]. Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness. -
序列相似性
Belongs to the heparin-binding growth factors family. -
翻译后修饰
Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.
N-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2254 Human
- Entrez Gene: 14180 Mouse
- Entrez Gene: 25444 Rat
- Omim: 600921 Human
- SwissProt: P31371 Human
- SwissProt: P54130 Mouse
- SwissProt: P36364 Rat
- Unigene: 111 Human
see all -
别名
- FGF 9 antibody
- FGF-9 antibody
- FGF9 antibody
see all
图片
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Western blot - Anti-FGF9/GAF antibody [EPR19937] - BSA and Azide free (ab223543)All lanes : Anti-FGF9/GAF antibody [EPR19937] (ab206408) at 1/1000 dilution
Lane 1 : Human FGF9/GAF active protein
Lane 2 : Human FGF16 recombinant protein
Lane 3 : Human FGF20 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaThis WB data was generated using the same anti-FGF9/GAF antibody clone [EPR19937] in a different buffer formulation (cat# ab206408).
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2: 1 seconds; Lane 3: 15 seconds.
Human FGF9/GAF active protein contains aa1-208; FGF16 and FGF20 recombinant protein contain aa1-203 and aa4-208 respectively, both with His-Tag®.
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![Anti-FGF9/GAF antibody [EPR19937] - BSA and Azide free (ab223543)](https://www.abcam.cn/ps/products/223/ab223543/Images/ab223543-2-benefits-of-recombinant-antibodies.png)
Anti-FGF9/GAF antibody [EPR19937] - BSA and Azide free (ab223543)
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab223543 尚未被引用在任何文献中。
