Anti-FGF22抗体(ab74860)
Key features and details
- Rabbit polyclonal to FGF22
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FGF22抗体 -
描述
兔多克隆抗体to FGF22 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide derived from internal sequence of human FGF22.
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阳性对照
- Human ovary tissue. Extracts from Jurkat cells and HT-29 cells. MCF-7 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74860于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 19 kDa.
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| IHC-P |
1/50 - 1/100.
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| ICC/IF |
1/500 - 1/1000.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 19 kDa. |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/500 - 1/1000. |
靶标
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功能
May be involved in hair development. -
序列相似性
Belongs to the heparin-binding growth factors family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 27006 Human
- Entrez Gene: 67112 Mouse
- Entrez Gene: 170579 Rat
- Omim: 605831 Human
- SwissProt: Q9HCT0 Human
- SwissProt: Q9ESS2 Mouse
- Unigene: 248087 Human
- Unigene: 154211 Mouse
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别名
- FGF 22 antibody
- FGF-22 antibody
- FGF22 antibody
see all
图片
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Western blot - Anti-FGF22 antibody (ab74860)All lanes : Anti-FGF22 antibody (ab74860) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells
Lane 2 : Extracts from HT-29 cells
Lane 3 : Extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 19 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 85 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF22 antibody (ab74860)Immunohistochemistry analysis of FGF22 in paraffin-embedded human ovary tissue using ab74860, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-FGF22 antibody (ab74860)Immunofluorescence analysis of FGF22 in MCF-7 cells using ab74860, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab74860 被引用在 2 文献中.
- Castilho ACS et al. Expression of fibroblast growth factor 22 (FGF22) and its receptor, FGFR1B, during development and regression of bovine corpus luteum. Theriogenology 125:1-5 (2019). PubMed: 30366151
- Castilho ACS et al. Evidence that fibroblast growth factor 10 plays a role in follicle selection in cattle. Reprod Fertil Dev 29:234-243 (2017). PubMed: 26194863