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使用敲除细胞株进行验证重组RabMAb

重组Anti-FGF21抗体[EPR8314(2)] - BSA and Azide free (ab271925)

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Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
  • Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
  • Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8314(2)] to FGF21 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC PE Unconjugated

概述

  • 产品名称

    Anti-FGF21抗体[EPR8314(2)] - BSA and Azide free
    参阅全部 FGF21 一抗

  • 描述

    兔单克隆抗体[EPR8314(2)] to FGF21 - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    The immunogen used for this product shares 6 continuous identical amino acids with SIKE1. Cross-reactivity with this protein has not been confirmed experimentally.

    Expression levels of the target protein vary with sample type and some optimisation may be required (PMID: 27285327).  For western blot using cell lines, it may be necessary to collect cell culture supernatant for endogenous FGF21 detection as the target protein is readily secreted (PMID: 24041694; PMID: 26691139).

    ab271925 is not recommended for mouse WB or human IHC-P.

  • 经测试应用

    适用于: IHC-P, WBmore details
    不适用于: ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556) can be used as a positive control in WB. WB: Human fetal liver lysate, rat spleen.

  • 常规说明

    ab271925 is the carrier-free version of ab171941.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271925于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
应用说明
Is unsuitable for ICC/IF.

靶标

图片

  • Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
    Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
    Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution + Human fetal liver tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171941).

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
    Western blot - Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
    Anti-FGF21 antibody [EPR8314(2)] (ab171941) at 1/5000 dilution + Rat spleen tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171941).

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)
    Anti-FGF21 antibody [EPR8314(2)] - BSA and Azide free (ab271925)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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