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重组RabMAb

重组Anti-FGF2抗体[EPR20145-219] - Low endotoxin，Azide free (ab222932)

Submit a review Submit a question References (1)
Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
  • Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
  • Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
  • Flow Cytometry (Intracellular) - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
  • Indirect ELISA - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
  • Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20145-219] to FGF2 - Low endotoxin, Azide free
  • Suitable for: Flow Cyt (Intra), Indirect ELISA, ICC/IF, IP, WB
  • Reacts with: Mouse, Human

概述

  • 产品名称

    Anti-FGF2抗体[EPR20145-219] - Low endotoxin，Azide free
    参阅全部 FGF2 一抗

  • 描述

    兔单克隆抗体[EPR20145-219] to FGF2 - Low endotoxin，Azide free

  • 宿主

    Rabbit

  • 特异性

    Mouse is only guaranteed in ELISA when detecting recombinant protein. We do not guarantee other applications and ELISA materials.

  • 经测试应用

    适用于: Flow Cyt (Intra), Indirect ELISA, ICC/IF, IP, WBmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Recombinant human FGF2 active protein (aa143-288); K562, U-87 MG and SK-OV-3 whole cell lysates; Human fetal kidney, prostate, fetal heart and testis lysates. ICC/IF: U-87 MG and SK-OV-3 cells. Flow Cyt (intra): K562 cells. IP: K562 whole cell lysate.

  • 常规说明

    ab222932 is the carrier-free version of ab208687.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab222932于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Indirect ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 24, 22, 18 kDa (predicted molecular weight: 30 kDa).
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Indirect ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 24, 22, 18 kDa (predicted molecular weight: 30 kDa).

靶标

  • 功能

    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).

  • 组织特异性

    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

  • 序列相似性

    Belongs to the heparin-binding growth factors family.

  • 翻译后修饰

    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

  • 细胞定位

    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Basic fibroblast growth factor antibody
      • Basic fibroblast growth factor bFGF antibody
      • BFGF antibody
      • FGF 2 antibody
      • FGF B antibody
      • FGF-2 antibody
      • Fgf2 antibody
      • FGF2 basic antibody
      • FGF2_HUMAN antibody
      • FGFB antibody
      • Fibroblast growth factor 2 (basic) antibody
      • Fibroblast growth factor 2 antibody
      • Fibroblast growth factor, basic antibody
      • HBGF 2 antibody
      • HBGF-2 antibody
      • HBGF2 antibody
      • HBGH 2 antibody
      • HBGH2 antibody
      • Heparin binding growth factor 2 precursor antibody
      • Heparin-binding growth factor 2 antibody
      • Prostatropin antibody
      see all

    图片

    • Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
      Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

      FGF2 was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab208687 at 1/30 dilution.

      Western blot was performed from the immunoprecipitate using ab208687 at 1/500 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: K562 whole cell lysate 10µg (Input).

      Lane 2: ab208687 IP in K562 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208687 in K562 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687).

    • Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
      Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing nuclear and weak cytoplasmic staining on U-87 MG cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687).

    • Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
      Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing nuclear weak cytoplasmic staining on SK-OV-3 cell line.

      The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687).

    • Flow Cytometry (Intracellular) - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
      Flow Cytometry (Intracellular) - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling  FGF2 with ab208687 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687). 

       

       

    • Indirect ELISA - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
      Indirect ELISA - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

      This data was developed using ab208687, the same antibody clone in a different buffer formulation.

      ELISA analysis of Mouse FGF2 recombinant protein at 1000 ng/ml with ab208687. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
    • Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
      Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

    文献 (1)

    发表研究结果有使用 ab222932？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab222932 被引用在 1 文献中.

    • Wang L  et al. Flavin containing monooxygenase 2 regulates renal tubular cell fibrosis and paracrine secretion via SMURF2 in AKI‑CKD transformation. Int J Mol Med 52:N/A (2023). PubMed: 37800598

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