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重组RabMAb

重组Anti-FGF2抗体[EP1735] (ab92337)

Reviews (2) Submit a question References (22)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
  • Immunoprecipitation - Anti-FGF2 antibody [EP1735] (ab92337)
  • Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
  • Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
  • Sandwich ELISA - Anti-FGF2 antibody [EP1735] (ab92337)
  • Anti-FGF2 antibody [EP1735] (ab92337)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1735] to FGF2
  • Suitable for: WB, IP, IHC-P, Sandwich ELISA
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

概述

  • 产品名称

    Anti-FGF2抗体[EP1735]
    参阅全部 FGF2 一抗

  • 描述

    兔单克隆抗体[EP1735] to FGF2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-P, Sandwich ELISAmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human FGF2 aa 100-200. The exact sequence is proprietary.

  • 阳性对照

    • WB: K-562, and HeLa lysates. IHC-P: human breast, prostate and cerebrum tissue. IP: HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab92337于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IP
1/10 - 1/100.
IHC-P (1)
1/2400.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AS24] to FGF basic (ab17279) as Capture.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IP
1/10 - 1/100.
IHC-P
1/2400.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AS24] to FGF basic (ab17279) as Capture.

靶标

  • 功能

    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).

  • 组织特异性

    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

  • 序列相似性

    Belongs to the heparin-binding growth factors family.

  • 翻译后修饰

    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

  • 细胞定位

    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Basic fibroblast growth factor antibody
      • Basic fibroblast growth factor bFGF antibody
      • BFGF antibody
      • FGF 2 antibody
      • FGF B antibody
      • FGF-2 antibody
      • Fgf2 antibody
      • FGF2 basic antibody
      • FGF2_HUMAN antibody
      • FGFB antibody
      • Fibroblast growth factor 2 (basic) antibody
      • Fibroblast growth factor 2 antibody
      • Fibroblast growth factor, basic antibody
      • HBGF 2 antibody
      • HBGF-2 antibody
      • HBGF2 antibody
      • HBGH 2 antibody
      • HBGH2 antibody
      • Heparin binding growth factor 2 precursor antibody
      • Heparin-binding growth factor 2 antibody
      • Prostatropin antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FGF2 with Purified ab92337 at 1:2400 dilution (0.51 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunoprecipitation - Anti-FGF2 antibody [EP1735] (ab92337)
      Immunoprecipitation - Anti-FGF2 antibody [EP1735] (ab92337)
      ab92337 (Purified) at 1:70 dilution (2 µg) immunoprecipitating FGF2 in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
      Lane 2 (+): ab92337 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92337 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.
    • Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
      Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
      Anti-FGF2 antibody [EP1735] (ab92337) at 1/5000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 17-30 kDa why is the actual band size different from the predicted?



      ab92337 recoginzes 3 isoforms of FGF2

    • Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
      Western blot - Anti-FGF2 antibody [EP1735] (ab92337)
      Anti-FGF2 antibody [EP1735] (ab92337) at 1/1000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 17-30 kDa why is the actual band size different from the predicted?



      ab92337 recoginzes 3 isoforms of FGF2

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

      Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

      Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Sandwich ELISA - Anti-FGF2 antibody [EP1735] (ab92337)
      Sandwich ELISA - Anti-FGF2 antibody [EP1735] (ab92337)

      Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1µg/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5µg/ml.

    • Anti-FGF2 antibody [EP1735] (ab92337)
      Anti-FGF2 antibody [EP1735] (ab92337)

    文献 (22)

    发表研究结果有使用 ab92337？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab92337 被引用在 22 文献中.

    • Jia X  et al. XBP1-elicited environment by chemotherapy potentiates repopulation of tongue cancer cells by enhancing miR-22/lncRNA/KAT6B-dependent NF-κB signalling. Clin Transl Med 13:e1166 (2023). PubMed: 36639835
    • Durgun C  et al. Investigation of the histopathological level of Ki-67, caspase-3 expressions of the effects of hesperidin on wound healing in the rat esophagus. Acta Cir Bras 38:e381723 (2023). PubMed: 37098927
    • Goudreau AD  et al. Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors. Int J Environ Res Public Health 20:N/A (2023). PubMed: 37444145
    • Shi Y  et al. Development and validation of a combined metabolism and immune prognostic model in lung adenocarcinoma. J Thorac Dis 14:4983-4997 (2022). PubMed: 36647508
    • Nirwana I  et al. Ellagic acid and hydroxyapatite promote angiogenesis marker in bone defect. J Oral Biol Craniofac Res 12:116-120 (2022). PubMed: 34840942
    View all Publications for this product

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FGF2 antibody [EP1735]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Flex TRS High pH
    Specification
    Kidney
    Blocking step
    (agent) for 5 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Mark Butters

    Verified customer

    提交于 Feb 02 2021

    Western blot abreview for Anti-FGF basic antibody [EP1735]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (HepG2)
    Specification
    HepG2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 19 2014

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