AB92337

Anti-FGF2 抗体 [EP1735]

Name: FGF2抗体[EP1735] (ab92337)| Abcam中文官网
Brand: Abcam Limited
SKU: AB92337
Price: 3183 CNY
Availability: InStock
Rating: 4 (2 reviews)

Anti-FGF2 antibody [EP1735]

RabMAb
Recombinant
了解详情

(2 Reviews)

(37 Publications)

Rabbit Recombinant Monoclonal FGF2 antibody. Suitable for IP, WB, sELISA, IHC-P and reacts with Human samples. Cited in 37 publications.

查看别名

FGFB, FGF2, Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, Heparin-binding growth factor 2, bFGF, HBGF-2

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (AB92337)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (AB92337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (AB92337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FGF2 with Purified ab92337 at 1 : 2400 dilution (0.51 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (AB92337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3 ?g/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Lab

Immunoprecipitation - Anti-FGF2 antibody [EP1735] (AB92337)

ab92337 (Purified) at 1 : 70 dilution (2 µg) immunoprecipitating FGF2 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+) : ab92337 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab92337 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92337)
ab92337 recoginzes 3 isoforms of FGF2

All lanes:

Immunoprecipitation - Anti-FGF2 antibody [EP1735] (ab92337)

Predicted band size: 30 kDa

false

Sandwich ELISA - Anti-FGF2 antibody [EP1735] (AB92337)

sELISA

Unknown

Sandwich ELISA - Anti-FGF2 antibody [EP1735] (AB92337)

Standard Curve for FGF basic (Analyte : FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1μg/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5μg/ml.

Lab

Western blot - Anti-FGF2 antibody [EP1735] (AB92337)

ab92337 recoginzes 3 isoforms of FGF2

All lanes:

Western blot - Anti-FGF2 antibody [EP1735] (ab92337) at 1/5000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

Observed band size: 17-30 kDa

false

Lab

Western blot - Anti-FGF2 antibody [EP1735] (AB92337)

ab92337 recoginzes 3 isoforms of FGF2

All lanes:

Western blot - Anti-FGF2 antibody [EP1735] (ab92337) at 1/1000 dilution

All lanes:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

Observed band size: 17-30 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP1735

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, sELISA, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.5 µg/mL", "sELISA-species-notes": "For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [AS24] to FGF basic (<a href='/products/unavailable/fgf-basic-antibody-as24-ab17279'>ab17279</a>) as Capture.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2400", "IHCP-species-notes": "Perform heat mediated antigen retrieval using <a href='/products/buffers/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab93684'>ab93684</a> (Tris/EDTA buffer, pH 9.0)." } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

FGF2 also known as FGF-2 or basic fibroblast growth factor is a protein that plays important roles in various cellular processes. It is a member of the fibroblast growth factor family and has a molecular weight of approximately 18 kDa. Scientists find FGF2 protein in many tissues including brain myocardium and bone. The protein can exist in different forms including recombinant variants. It interacts with cell surface receptors to exert its effects significantly influencing cell behavior.

Biological function summary

Various functions characterize FGF2 such as cell proliferation differentiation and migration. It does not act alone but is part of a larger protein complex. This complex includes FGF receptors which help mediate the binding of the FGF2 peptide ensuring proper signaling within the cells. FGF2 is important for development and repair processes making it an essential factor in tissue maintenance and regeneration.

Pathways

FGF2 plays a big role in critical pathways like the MAPK/ERK signaling pathway and PI3K/Akt pathway. These pathways help regulate the processes of cell growth and survival. FGF2 interacts with other protein molecules such as FGFR1 and FGFR2 which serve as receptors to propagate the signaling cascade initiated by FGF2 binding. Its interaction in these pathways underlines its role in modulating important cellular responses.

Scientists connect FGF2 with conditions such as cancer and atherosclerosis. The protein's deregulation may lead to abnormal cell proliferation contributing to tumor growth and vascular diseases. FGF2's interaction with proteins like FGFR1 can influence cancer progression and plaque formation in blood vessels. By understanding FGF2's role in these diseases researchers aim to develop targeted therapies that can potentially alter disease outcomes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed : 8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed : 28302677). Binds to integrin ITGAV : ITGB3 (PubMed : 28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed : 28302677, PubMed : 8663044). Functions as a potent mitogen in vitro (PubMed : 1721615, PubMed : 3732516, PubMed : 3964259). Can induce angiogenesis (PubMed : 23469107, PubMed : 28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed : 29501879).

See full target information FGF2

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文献 (37)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:9669 PubMed40113924

2025

CD206 and dust particles are prognostic biomarkers of progressive fibrosing interstitial lung disease associated with air pollutant exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Aliaksei Kadushkin,Olga Yudina,Nastassia Lukashevich,Elena Davidovskaya,Vasyl Filanyuk,Volha Dziadzichkina,Xiaoming Cai

Heliyon 10:e39843 PubMed39553576

2024

The effect of fibroblast growth factor 2 on neovascular vessels depends on the stage of angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Hattori,Haruhiko Yamada,Hidetsugu Mori,Shinpei Oba,Kaito Yokota,Masatoshi Omi,Yuichi Yamamoto,Keiko Toyama,Masayuki Ohnaka,Kanji Takahashi,Hisanori Imai

Heliyon 10:e34243 PubMed39091947

2024

Long non-coding RNA MIMT1 promotes retinoblastoma proliferation via sponging miR-153-5p to upregulate FGF2.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Ruyuan Cai,Tingting Sun,Zhufang Yang,Hongjie Zhang

Biomaterials research 28:0020 PubMed38715911

2024

Engineered Basic Fibroblast Growth Factor Specifically Bonded with Injectable Extracellular Matrix Hydrogel for the Functional Restoration of Cerebral Ischemia in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Chunying Shi,Qi Liu,Feng Sun,Guangyu Zhang,Mingru Deng,Bo Xu,Haicheng Yuan

Journal of biomedical materials research. Part B, Applied biomaterials 112:e35403 PubMed38520706

2024

Improving osseointegration and antimicrobial properties of titanium implants with black phosphorus nanosheets-hydroxyapatite composite coatings for vascularized bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shilong Ma,Ruize Sun,Yuhui Wang,Yan Wei,Haofeng Xu,Xuanyu Liu,Ziwei Liang,Liqin Zhao,Yinchun Hu,Xiaojie Lian,Meiqing Guo,Di Huang

Acta cirurgica brasileira 39:e391224 PubMed38477786

2024

Ellagic acid protected the gingival tissue via fibroblast and epidermal growth factors in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Gülüçağ Giray Tekin,Buşra Deveci,Engin Deveci

The Journal of clinical endocrinology and metabolism 109:2306-2316 PubMed38421044

2024

An Orally Efficacious Thyrotropin Receptor Ligand Inhibits Growth and Metastatic Activity of Thyroid Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Rhitajit Sarkar,Priyanka Bolel,Abhijeet Kapoor,Elena Eliseeva,Andrés E Dulcey,Jay S Templin,Amy Q Wang,Xin Xu,Noel Southall,Joanna Klubo-Gwiezdzinska,Susanne Neumann,Juan J Marugan,Marvin C Gershengorn

International journal of environmental research and public health 20: PubMed37444145

2023

Examining the Effects of Gestational Physical Activity and Hofbauer Cell Polarization on Angiogenic Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra D Goudreau,Layli Tanara,Velislava Tzaneva,Kristi B Adamo

Acta cirurgica brasileira 38:e381723 PubMed37098927

2023

Investigation of the histopathological level of Ki-67, caspase-3 expressions of the effects of hesperidin on wound healing in the rat esophagus.

Applications

Unspecified application

Species

Unspecified reactive species

Cemalettin Durgun,Gülsen Kirman,Engin Deveci

Pharmaceutical biology 61:581-589 PubMed36994813

2023

Vitexin suppresses the proliferation, angiogenesis and stemness of endometrial cancer through the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cuixia Liang,Yongjie Jiang,Lizhu Sun

View all publications

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For licensing inquiries, please contact partnerships@abcam.com

Anti-FGF2 抗体 [EP1735]

Anti-FGF2 antibody [EP1735]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (AB92337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (AB92337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (AB92337)

Immunoprecipitation - Anti-FGF2 antibody [EP1735] (AB92337)

Sandwich ELISA - Anti-FGF2 antibody [EP1735] (AB92337)

Western blot - Anti-FGF2 antibody [EP1735] (AB92337)

Western blot - Anti-FGF2 antibody [EP1735] (AB92337)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原