重组Anti-FGF1抗体[EPR19989] - BSA and Azide free (ab271978)
![Western blot - Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)](https://www.abcam.cn/ps/products/271/ab271978/Images/ab271978-386692-antifgf1antibodyepr19989westernblot-2.jpg "Western blot - Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19989] to FGF1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-FGF1抗体[EPR19989] - BSA and Azide free
参阅全部 FGF1 一抗 -
描述
兔单克隆抗体[EPR19989] to FGF1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal kidney, fetal brain and glioma lysates; U-87 MG and Neuro-2a whole cell lysates; Mouse spinal cord, heart and kidney lysates; Rat heart lysate.
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常规说明
ab271978 is the carrier-free version of ab207321.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19989 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271978于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa. |
靶标
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功能
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
序列相似性
Belongs to the heparin-binding growth factors family. -
细胞定位
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
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数据库链接
- Entrez Gene: 2246 Human
- Entrez Gene: 14164 Mouse
- Entrez Gene: 25317 Rat
- Omim: 131220 Human
- SwissProt: P05230 Human
- SwissProt: P61148 Mouse
- SwissProt: P61149 Rat
- Unigene: 483635 Human
see all -
别名
- Acidic fibroblast growth factor antibody
- aFGF antibody
- Beta endothelial cell growth factor antibody
see all
图片
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Western blot - Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)All lanes : Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution
Lane 1 : Human glioma lysate
Lane 2 : Mouse spinal cord lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2/5: 3 minutes; Lane 3: 10 seconds; Lane 4: 15 seconds.
The observed molecular weight is consistent with the literature (PMID: 16091423).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207321).
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![Western blot - Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)](https://www.abcam.cn/ps/products/271/ab271978/Images/ab271978-386691-antifgf1antibodyepr19989westernblot.jpg)
Western blot - Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)All lanes : Anti-FGF1 antibody [EPR19989] (ab207321) at 1/1000 dilution
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1/2/5: 3 minutes; Lane 3: 10 seconds; Lane 4: 15 seconds.
The observed molecular weight is consistent with the literature (PMID: 16091423).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207321).
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![Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)](https://www.abcam.cn/ps/products/271/ab271978/Images/ab271978-3-benefits-of-recombinant-antibodies.png)
Anti-FGF1 antibody [EPR19989] - BSA and Azide free (ab271978)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab271978 尚未被引用在任何文献中。
