重组Anti-Ferritin Light Chain抗体[EPR5260] (ab109373)
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.cn/ps/products/109/ab109373/Images/ab109373-389695-anti-ferritin-light-chain-antibody-epr5260-western-blot-human-lysate.jpg "Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5260] to Ferritin Light Chain
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Ferritin Light Chain抗体[EPR5260]
参阅全部 Ferritin Light Chain 一抗 -
描述
兔单克隆抗体[EPR5260] to Ferritin Light Chain -
宿主
Rabbit -
特异性
This antibody recognizes the light chain of Ferritin only.
-
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Human fetal liver, Hap1, HepG2 and HeLa cell lysates. Flow Cyt (intra): HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5260 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free (ab239971)
- Alexa Fluor® 488 Anti-Ferritin Light Chain antibody [EPR5260] (ab320378)
- Alexa Fluor® 647 Anti-Ferritin Light Chain antibody [EPR5260] (ab320379)
- PE Anti-Ferritin Light Chain antibody [EPR5260] (ab320380)
- APC Anti-Ferritin Light Chain antibody [EPR5260] (ab320381)
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109373于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 20 kDa). |
靶标
-
功能
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
疾病相关
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
序列相似性
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 2512 Human
- Entrez Gene: 14325 Mouse
- Entrez Gene: 29292 Rat
- Omim: 134790 Human
- SwissProt: P02792 Human
- SwissProt: P29391 Mouse
- SwissProt: P02793 Rat
- Unigene: 433670 Human
see all -
别名
- Ferritin L chain antibody
- Ferritin L subunit antibody
- Ferritin light chain antibody
see all
图片
-
Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/2000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : Ferritin knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDaLanes 1-2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109373 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265533 (knockout cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. ab109373 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.cn/ps/products/109/ab109373/Images/ab109373-319298-anti-ferritin-light-chain-antibody-epr5260-western-blot.jpg)
Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 20 kDaLanes 1 - 2: Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab109373 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. Ab109373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.cn/ps/products/109/ab109373/Images/ab109373-122150-anti-ferritin-light-chain-antibody-epr5260-western-blot.jpg)
Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)All lanes : Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 20 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.cn/ps/products/109/ab109373/Images/ab109373-122149-anti-ferritin-light-chain-antibody-epr5260-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)Intracellular flow cytometric analysis of permeabilized HeLa cells using ab109373 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
-
![Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)](https://www.abcam.cn/ps/products/109/ab109373/Images/ab109373-6-benefits-of-recombinant-antibodies.png)
Anti-Ferritin Light Chain antibody [EPR5260] (ab109373)
数据表及文件
-
SDS download
-
Datasheet download
文献 (22)
ab109373 被引用在 22 文献中.
- Yang XY et al. Huperzine A-Liposomes Efficiently Improve Neural Injury in the Hippocampus of Mice with Chronic Intermittent Hypoxia. Int J Nanomedicine 18:843-859 (2023). PubMed: 36824413
- Han Y et al. CHIR99021 Maintenance of the Cell Stemness by Regulating Cellular Iron Metabolism. Antioxidants (Basel) 12:N/A (2023). PubMed: 36829936
- Zheng H et al. Cdh5-mediated Fpn1 deletion exerts neuroprotective effects during the acute phase and inhibitory effects during the recovery phase of ischemic stroke. Cell Death Dis 14:161 (2023). PubMed: 36841833
- An JR et al. Effects of liraglutide on astrocyte polarization and neuroinflammation in db/db mice: focus on iron overload and oxidative stress. Front Cell Neurosci 17:1136070 (2023). PubMed: 37323581
- Li H et al. Ferritin light chain promotes the reprogramming of glioma immune microenvironment and facilitates glioma progression. Theranostics 13:3794-3813 (2023). PubMed: 37441589