AB109373

重组Anti-Ferritin Light Chain抗体[EPR5260]

Anti-Ferritin Light Chain antibody [EPR5260]

RabMAb
Recombinant
KO Validated
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(43 Publications)

Rabbit Recombinant Monoclonal Ferritin Light Chain antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 43 publications.

查看别名

Ferritin light chain, Ferritin L subunit, FTL

3 Images

Lab

Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (AB109373)

Lanes 1 - 2 : Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109373 was shown to recognize Ferritin Light Chain in wild-type HAP1 cells as signal was lost at the expected MW in FTL (Ferritin Light Chain) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FTL (Ferritin Light Chain) knockout samples were subjected to SDS-PAGE. ab109373 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

FTL (Ferritin Light Chain) knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 20 kDa,49 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (AB109373)

Lanes 1-2 : Merged signal (red and green). Green - ab109373 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.

ab109373 Anti-FTL was shown to specifically react with Ferritin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265533 (knockout cell lysate ab256926) was used. Wild-type and FTL knockout samples were subjected to SDS-PAGE. ab109373 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Ferritin knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FTL knockout HeLa cell line (<a href='/products/cell-lines/human-ftl-knockout-hela-cell-line-ab265533'>ab265533</a>)

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Unknown

Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (AB109373)

All lanes:

Western blot - Anti-Ferritin Light Chain antibody [EPR5260] (ab109373) at 1/10000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 20 kDa

Observed band size: 19 kDa

false

不同偶联物与剂型 (5)

Carrier free

Anti-Ferritin Light Chain antibody [EPR5260] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Ferritin Light Chain antibody [EPR5260]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Ferritin Light Chain antibody [EPR5260]
578 PE

PE Anti-Ferritin Light Chain antibody [EPR5260]
660 APC

APC Anti-Ferritin Light Chain antibody [EPR5260]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5260

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody recognizes the light chain of Ferritin only.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed : 24695223).

See full target information FTL

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文献 (43)

Recent publications for all applications. Explore the full list and refine your search

eLife 14: PubMed41037321

2025

Lipid peroxidation and type I interferon coupling fuels pathogenic macrophage activation causing tuberculosis susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

Shivraj M Yabaji,Vadim Zhernovkov,Prasanna Babu Araveti,Suruchi Lata,Oleksii S Rukhlenko,Salam Al Abdullatif,Arthur Vanvalkenburg,Yuriy O Alekseyev,Qicheng Ma,Gargi Dayama,Nelson C Lau,W Evan Johnson,William R Bishai,Nicholas A Crossland,Joshua D Campbell,Boris N Kholodenko,Alexander A Gimelbrant,Lester Kobzik,Igor Kramnik

International journal of nanomedicine 20:7533-7548 PubMed40546801

2025

Platelet Membrane-Based Nanoparticles for Targeted Delivery of Deferoxamine to Alleviate Brain Injury Induced by Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Peina Wang,Xin Lv,Siyu Tian,Wen Yang,Mudi Feng,Shiyang Chang,Linhao You,Yan-Zhong Chang

Cell death & disease 16:366 PubMed40328750

2025

ZIP8 modulates ferroptosis to drive esophageal carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaojie Yang,Kexin Zhao,Xiangping Li,Ruoping Yanzhang,Huijun Zhang,Yin Yu,Mingyang Yan,Shaobo Fang,Tao Li,Hao Li,Xiao Chu,Siyuan Han,Ziliang Zhang,Junyan Teng,Guoguo Jin,Zhiping Guo

CNS neuroscience & therapeutics 31:e70369 PubMed40202153

2025

Sevoflurane Inhibits the Proliferation of Neural Precursor Cells and Neural Migration of Mice by Inducing Iron Metabolism Disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Xincheng Li,Runjiao Cheng,Mahammad Naeem,Xiaoou Nie,Jiaqi Wang,Liqiang Zhao,Xiaopeng Liu,Zhenhua Shi,Jianhua Zhang

Redox biology 82:103611 PubMed40184642

2025

Absence of astrocytic ceruloplasmin reverses the senescence process with aging of learning and memory abilities.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Da Li,Shaomeng Kang,Haiyan Li,Peng Yu,Ruikun Xie,Chenchen Li,Qi Jing,Zhengzheng Gong,Li Li,Zhengning Li,Mengyu Geng,Zihan Zhang,Yang Li,Yan-Zhong Chang

Kidney international reports 9:1473-1483 PubMed38707804

2024

Kidney Tissue Proteome Profiles in Short Versus Long Duration of Delayed Graft Function - A Pilot Study in Donation After Circulatory Death Donors.

Applications

Unspecified application

Species

Unspecified reactive species

M Letizia Lo Faro,Kaithlyn Rozenberg,Honglei Huang,Sergei Maslau,Sarah Bonham,Roman Fischer,Benedikt Kessler,Henri Leuvenink,Edward Sharples,Jan H Lindeman,Rutger Ploeg

Clinical and translational medicine 14:e1653 PubMed38616702

2024

Mitochondrial dysfunction induced by HIF-1α under hypoxia contributes to the development of gastric mucosal lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Yuelin Xiao,Xianzhi Liu,Kaiduan Xie,Jiajie Luo,Yiwang Zhang,Xiaoli Huang,Jinni Luo,Siwei Tan

Scientific reports 14:7889 PubMed38570541

2024

Nobiletin alleviates cisplatin-induced ototoxicity via activating autophagy and inhibiting NRF2/GPX4-mediated ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenao Song,Li Zhang,Xiaolin Cui,Rongrong Wang,Jingyu Ma,Yue Xu,Yan Jin,Dawei Wang,Zhiming Lu

iScience 27:109219 PubMed38469561

2024

Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mary E Mohr,Shuang Li,Allison M Trouten,Rebecca A Stairley,Patrick L Roddy,Chun Liu,Min Zhang,Henry M Sucov,Ge Tao

Cell stem cell 31:378-397.e12 PubMed38402617

2024

An iron rheostat controls hematopoietic stem cell fate.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Ruei Kao,Jiahao Chen,Rajni Kumari,Anita Ng,Aliona Zintiridou,Madhuri Tatiparthy,Yuhong Ma,Maria M Aivalioti,Deeposree Moulik,Sriram Sundaravel,Daqian Sun,Julie A Reisz,Juliane Grimm,Nuria Martinez-Lopez,Stephanie Stransky,Simone Sidoli,Ulrich Steidl,Rajat Singh,Angelo D'Alessandro,Britta Will

View all publications

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