重组Anti-Ferritin Heavy Chain抗体[EPR3005Y] (ab75972)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3005Y] to Ferritin Heavy Chain
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Ferritin Heavy Chain抗体[EPR3005Y]
参阅全部 Ferritin Heavy Chain 一抗 -
描述
兔单克隆抗体[EPR3005Y] to Ferritin Heavy Chain -
宿主
Rabbit -
特异性
This antibody recognizes only the Ferritin heavy chain. -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Ferritin Heavy Chain aa 150-250 (C terminal). The exact sequence is proprietary.
Database link: P02794 -
阳性对照
- WB: Human fetal liver, SH-SY5Y, human brain and HeLa cell lysates. IHC: Human kidney, breast and tonsil tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3005Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Anti-Ferritin Heavy Chain antibody [EPR3005Y] - BSA and Azide free (ab247418)
- APC Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab319237)
- PE Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab319385)
- Alexa Fluor® 488 Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab319519)
- Alexa Fluor® 647 Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab319632)
- Alexa Fluor® 594 Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab319736)
- Alexa Fluor® 555 Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab319878)
- Alexa Fluor® 750 Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab321705)
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75972于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
靶标
- Information by UniProt
-
数据库链接
- Entrez Gene: 2495 Human
- Omim: 134770 Human
- SwissProt: P02794 Human
- Unigene: 524910 Human
- Unigene: 645560 Human
-
别名
- Apoferritin antibody
- Cell proliferation inducing gene 15 protein antibody
- Cell proliferation-inducing gene 15 protein antibody
see all
图片
-
All lanes : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/1000 dilution
Lane 1 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysates prepared from 1% SDS Hot lysis method.
Lane 2 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysates prepared from RIPA lysis method
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates prepared from 1% SDS Hot lysis method
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates prepared from RIPA lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
Ab75972 works better in some lysates prepared from 1% SDS Hot lysis method.
For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). -
ab75972 staining Ferritin Heavy Chain in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
-
Lanes 1-3 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/500 dilution (unpurified)
Lane 4 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution (unpurified)
Lane 5 : Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution (unpurified)
Lane 1 : Human liver lysate
Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate
Lanes 3-4 : Human brain lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/5000 dilution + Human fetal liver at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 21 kDa -
Anti-Ferritin Heavy Chain antibody [EPR3005Y] (ab75972) at 1/2000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa -
Unpurified ab75972 at 1/100 dilution staining Ferritin Heavy Chain in Human kidney tissue, using a HRP/AP polymerized secondary antibody.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
Unpurified ab75972 showing positive staining in normal liver tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
Unpurified ab75972 showing positive staining in normal breast tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
Unpurified ab75972 showing positive staining in normal tonsil tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
数据表及文件
-
SDS download
-
Datasheet download
文献 (39)
ab75972 被引用在 39 文献中.
- Wei X et al. Defective NCOA4-dependent ferroptosis in senescent fibroblasts retards diabetic wound healing. Cell Death Discov 9:138 (2023). PubMed: 37117222
- Lv H et al. A static magnetic field improves bone quality and balances the function of bone cells with regulation on iron metabolism and redox status in type 1 diabetes. FASEB J 37:e22985 (2023). PubMed: 37249350
- Lu ZL et al. Hydroxycitric Acid Alleviated Lung Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress and Ferroptosis through the Hif-1α Pathway. Curr Issues Mol Biol 45:9868-9886 (2023). PubMed: 38132462
- Li H et al. Combining single-cell RNA sequencing and population-based studies reveals hand osteoarthritis-associated chondrocyte subpopulations and pathways. Bone Res 11:58 (2023). PubMed: 37914703
- Tong W et al. Sensitive magnetic particle imaging of haemoglobin degradation for the detection and monitoring of intraplaque haemorrhage in atherosclerosis. EBioMedicine 90:104509 (2023). PubMed: 36905783