重组Anti-FEN1抗体[EPR4460(2)] (ab109132)
![Western blot - Anti-FEN1 antibody [EPR4460(2)] (ab109132)](https://www.abcam.cn/ps/products/109/ab109132/Images/ab109132-121735-anti-fen1-antibody-epr44602-western-blot.jpg "Western blot - Anti-FEN1 antibody [EPR4460(2)] (ab109132)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4460(2)] to FEN1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FEN1抗体[EPR4460(2)]
参阅全部 FEN1 一抗 -
描述
兔单克隆抗体[EPR4460(2)] to FEN1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab95382) -
阳性对照
- Jurkat, HeLa, Y79, and Daudi cell lysates, Human testis, Human tonsil, and Human urothelial carcinoma tissues
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4460(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109132于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Recombinant Human FEN1 protein (ab95382).
|
|
| IHC-P |
1/500 - 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Recombinant Human FEN1 protein (ab95382). |
|
IHC-P
1/500 - 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. -
序列相似性
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily. -
翻译后修饰
Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. -
细胞定位
Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 2237 Human
- Entrez Gene: 14156 Mouse
- Entrez Gene: 84490 Rat
- Omim: 600393 Human
- SwissProt: P39748 Human
- SwissProt: P39749 Mouse
- SwissProt: Q5XIP6 Rat
- SwissProt: Q9JHW7 Rat
see all -
别名
- DNase IV antibody
- FEN-1 antibody
- FEN1 antibody
see all
图片
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Western blot - Anti-FEN1 antibody [EPR4460(2)] (ab109132)All lanes : Anti-FEN1 antibody [EPR4460(2)] (ab109132) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Y79 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)](https://www.abcam.cn/ps/products/109/ab109132/Images/ab109132-121734-anti-fen1-antibody-epr44602-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109132 at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)](https://www.abcam.cn/ps/products/109/ab109132/Images/ab109132-121733-anti-fen1-antibody-epr44602-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109132 at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)](https://www.abcam.cn/ps/products/109/ab109132/Images/ab109132-121732-anti-fen1-antibody-epr44602-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (ab109132)Immunohistochemical analysis of paraffin-embedded Human urothelial carcinoma tissue using ab109132 at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-FEN1 antibody [EPR4460(2)] (ab109132)](https://www.abcam.cn/ps/products/109/ab109132/Images/ab109132-5-benefits-of-recombinant-antibodies.png)
Anti-FEN1 antibody [EPR4460(2)] (ab109132)
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab109132 被引用在 11 文献中.
- Gerdtsson AS et al. Overexpression of the key metabolic protein CPT1A defines mantle cell lymphoma patients with poor response to standard high-dose chemotherapy independent of MIPI and complement established highrisk factors. Haematologica 108:1092-1104 (2023). PubMed: 36519324
- Bona N & Crossan GP Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development. Nat Struct Mol Biol 30:1434-1445 (2023). PubMed: 37580626
- Zhou J et al. Proteomic Analyses Identify Differentially Expressed Proteins and Pathways Between Low-Risk and High-Risk Subtypes of Early-Stage Lung Adenocarcinoma and Their Prognostic Impacts. Mol Cell Proteomics 20:100015 (2021). PubMed: 33508502
- Cong K et al. Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency. Mol Cell 81:3128-3144.e7 (2021). PubMed: 34216544
- Huang W et al. Jianpi-yangwei decoction inhibits DNA damage repair in the drug resistance of gastric cancer by reducing FEN1 expression. BMC Complement Med Ther 20:196 (2020). PubMed: 32586310