Anti-FE65抗体

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FE65 antibody (AB5668)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized, 70% confluent log phase SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells, blocked with 1% BSA for 1 hour at room temperature. Labelling Fe65 with ab5668 at 2 μg/mL and incubated for 3 hours at room temperature and then labelled with Goat anti-Rabbit IgG secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (Blue). F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e shows the no primary antibody control. The images were captured at 60X magnification.

• WB

Unknown

Western blot - Anti-FE65 antibody (AB5668)

Western blot of Fe65-affinity pulldown materials from mouse brain lysate using ab5668. Lane 1 is an immunopurified sample from a full-length Fe65 Knockout mouse. Lane 2 is an immunopurified sample from an Fe65 wild type mouse.

All lanes:

Western blot - Anti-FE65 antibody (ab5668)

Predicted band size: 77 kDa

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• WB

Supplier Data

Western blot - Anti-FE65 antibody (AB5668)

Detection : chemiluminescence

All lanes:

Western blot - Anti-FE65 antibody (ab5668) at 2 µg/mL

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg with blocking with 5 % skimmed milk

Lane 2:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 30 µg with blocking with 5 % skimmed milk

Lane 3:

NTERA-2 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg with blocking with 5 % skimmed milk

Lane 4:

U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 30 µg with blocking with 5 % skimmed milk

Lane 5:

A549 (Human lung carcinoma cell line) whole cell lysate at 30 µg with blocking with 5 % skimmed milk

Lane 6:

Mouse Brain at 30 µg with blocking with 5 % skimmed milk

Lane 7:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg with blocking with 5 % skimmed milk

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit IgG at 1/2500 dilution

Predicted band size: 77 kDa

Observed band size: ~77 kDa

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