AB109007

重组Anti-FDPS/FPS抗体[EPR4628]

Anti-FDPS/FPS antibody [EPR4628]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal FDPS/FPS antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

FPS, KIAA1293, FDPS, Farnesyl pyrophosphate synthase, FPP synthase, Dimethylallyltranstransferase, Farnesyl diphosphate synthase, Geranyltranstransferase

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDPS/FPS antibody [EPR4628] (AB109007)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDPS/FPS antibody [EPR4628] (AB109007)

ab109007 at 1/100 dilution staining FDPS/FPS in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-FDPS/FPS antibody [EPR4628] (AB109007)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-FDPS/FPS antibody [EPR4628] (AB109007)

Flow Cytometry analysis of THP-1 (Human monocytic leukemia monocyte) cells labeling FDPS/FPS with purified ab109007 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Unknown

Western blot - Anti-FDPS/FPS antibody [EPR4628] (AB109007)

All lanes:

Western blot - Anti-FDPS/FPS antibody [EPR4628] (ab109007) at 1/1000 dilution

Lane 1:

THP1 cell lysates

Lane 2:

HepG2 cell lysates

Lane 3:

Human fetal liver lysates

Predicted band size: 48 kDa

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4628

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Farnesyl diphosphate synthase (FDPS) also known as farnesyl pyrophosphate synthase (FPS) is an important enzyme that catalyzes the synthesis of farnesyl diphosphate (FPP) from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). This enzyme plays an important role in the mevalonate pathway which is essential for the biosynthesis of sterols including cholesterol and other isoprenoids. FDPS is a protein with a molecular mass of approximately 42 kDa. Its expression occurs in various tissues with significant levels in liver brain and bone where it contributes to different cellular metabolic processes.

Biological function summary

Farnesyl diphosphate synthase facilitates the production of FPP a common precursor for the synthesis of isoprenoid compounds such as cholesterol ubiquinones and dolichol. FDPS does not form a complex but works in coordination with other enzymes in the mevalonate pathway. Its activity supports numerous cellular functions including membrane assembly protein prenylation and cell signaling. The enzyme’s role is important for normal cellular growth and development influencing processes like cell differentiation and proliferation.

Pathways

FDPS plays a significant role in the cholesterol biosynthesis and protein prenylation pathways. These pathways are critical for cellular homeostasis and involve several enzymes including HMG-CoA reductase and squalene synthase. The cholesterol biosynthesis pathway includes multiple steps where FPP serves as a lipid intermediate. Meanwhile in protein prenylation FPP contributes to the post-translational modification of proteins such as small GTPases which impacts cell signaling and cytoskeletal organization.

FDPS has a connection to bone diseases such as osteoporosis and also links to cardiovascular disorders. The relationship to osteoporosis arises from its role in bone resorption and osteoclast activity mediated by proteins like RANKL and osteoprotegerin. Cardiovascular disorders may link to altered cholesterol metabolism with FDPS influencing cholesterol biosynthesis and regulation. Inhibition of FDPS activity targets these pathways leading to therapeutic strategies that may address both metabolic and bone health issues.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.

See full target information FDPS

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical immunology 45:139 PubMed41026257

2025

Homozygous Loss of Function PIK3CD Mutation in Multiple Siblings Leading To B Cell Dysregulation and Autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Huda Alajlan,Amer Al-Mazrou,Hibah Alruwaili,Safia Sumayli,Ali Almehaidib,Khalid Alsaleem,Sawsan Abu Awwad,Hazem Ghebeh,Monther Al-Alwan,Anas M Alazami,Hamoud Al-Mousa

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-FDPS/FPS抗体[EPR4628]

Anti-FDPS/FPS antibody [EPR4628]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FDPS/FPS antibody [EPR4628] (AB109007)

Flow Cytometry (Intracellular) - Anti-FDPS/FPS antibody [EPR4628] (AB109007)

Western blot - Anti-FDPS/FPS antibody [EPR4628] (AB109007)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原