重组Anti-FCGRT/FCRN抗体[EPR22627-25] (ab228975)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22627-25] to FCGRT/FCRN
- Suitable for: IHC-P, WB, IP, Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-FCGRT/FCRN抗体[EPR22627-25]
参阅全部 FCGRT/FCRN 一抗 -
描述
兔单克隆抗体[EPR22627-25] to FCGRT/FCRN -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IP, Flow Cytmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type HEK-293T (non-ATCC), HEK-293 (ATCC), HEK-293 and THP-1 cell lysates; Human placenta and placenta treated with PNGase F tissue lysates. IHC-P: Human spleen and liver tissue. Flow Cyt: THP-1 cells. IP: HEK-293 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22627-25 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab228975于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 40 kDa.
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IP |
1/30.
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Flow Cyt |
1/500.
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说明 |
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IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 40 kDa. |
IP
1/30. |
Flow Cyt
1/500. |
靶标
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功能
Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the uptake of IgG from milk. Possible role in transfer of immunoglobulin G from mother to fetus. -
序列相似性
Belongs to the immunoglobulin superfamily. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2217 Human
- Omim: 601437 Human
- SwissProt: P55899 Human
- Unigene: 111903 Human
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别名
- Alpha chain antibody
- Fc fragment of IgG, receptor transporter, alpha antibody
- FCGRN_HUMAN antibody
see all
图片
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All lanes : Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (non-ATCC) cell lysate
Lane 2 : Human FCGRT (FCRN) knockout HEK-293T (non-ATCC) cell lysate (ab257435)
Lane 3 : HEK293 (ATCC) cell lysate
Lane 4 : LNCaP cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDaLanes 1-4: Merged signal (red and green). Green - ab228975 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab228975 Anti-FCGRT/FCRN antibody [EPR22627-25] was shown to specifically react with FCGRT/FCRN in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab265075 (knockout cell lysate ab257435) was used. Wild-type and FCGRT/FCRN knockout samples were subjected to SDS-PAGE. ab228975 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human liver (PMID: 28402755) is observed. The section was incubated with ab228975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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All lanes : Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human placenta treated with PNGase F tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 40 kDa
Exposure time: 3 minutesBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
Human FCGRT/FCRN possesses one potential glycosylation site. The molecular weight of deglycosylated FCGRT/FCRN is consistent with the literature (PMID: 12163559).
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All lanes : Anti-FCGRT/FCRN antibody [EPR22627-25] (ab228975) at 1/1000 dilution
Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : LNCaP (human prostate carcinoma epithelial cell), whole cell lysate
Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lane 1: 37 secs; Lane 2-4: 3 mins.
Negative control: LNCaP (PMID: 27974681) and HeLa (PMID: 21216798).
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FCGRT/FCRN was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab228975 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab228975 1/1000 dilution (0.55 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2: ab228975 IP in HEK-293 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab228975 in HEK-293 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 100 seconds.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen, arrows indicate the macrophages (PMID: 28402755) is observed .The section was incubated with ab228975 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling FCGRT/FCRN with ab228975 at 1/4000 dilution (0.14 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of human spleen (PMID: 28402755) is observed. The section was incubated with ab228975 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / THP-1 (human monocytic leukemia monocyte, Right) cells labeling FCGRT/FCRN with ab228975 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Bright fluorophore (like AF647) conjugated secondary antibodies are recommended to get good separation.
Negative control: HeLa (PMID: 21216798).
Gated on viable cells.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab228975 被引用在 1 文献中.
- Guan X et al. Activation of FcRn Mediates a Primary Resistance Response to Sorafenib in Hepatocellular Carcinoma by Single-Cell RNA Sequencing. Front Pharmacol 12:709343 (2021). PubMed: 34421602