Anti-FBXO3抗体(ab224603)
Key features and details
- Rabbit polyclonal to FBXO3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-FBXO3抗体 -
描述
兔多克隆抗体to FBXO3 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human FBXO3 aa 276-376.
Database link: Q9UK99 -
阳性对照
- IHC-P: Human skeletal muscle tissue. ICC/IF: HeLa cells. WB: Mouse kidney tissue lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224603于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/200.
|
|
| WB |
1/200 - 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
|
|
| IHC-P |
1/20 - 1/200.
|
| 说明 |
|---|
|
ICC/IF
1/50 - 1/200. |
|
WB
1/200 - 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
|
IHC-P
1/20 - 1/200. |
靶标
-
功能
Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. -
序列相似性
Contains 1 apaG domain.
Contains 1 F-box domain. -
细胞定位
Nucleus. Colocalizes with PML at the peripheries of nuclear bodies. - Information by UniProt
-
数据库链接
- Entrez Gene: 26273 Human
- Entrez Gene: 57443 Mouse
- Omim: 609089 Human
- SwissProt: Q9UK99 Human
- SwissProt: Q9DC63 Mouse
- Unigene: 406787 Human
- Unigene: 143768 Mouse
-
别名
- F Box G - Domain 3 antibody
- F box only protein 3 antibody
- F box protein 3 antibody
see all
图片
-
Western blot - Anti-FBXO3 antibody (ab224603)Anti-FBXO3 antibody (ab224603) at 1/200 dilution + Mouse kidney tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO3 antibody (ab224603)Paraffin-embedded human skeletal muscle tissue stained for FBXO3 using ab224603 at 1/100 dilution in immunohistochemical analysis.
-
Immunocytochemistry/ Immunofluorescence - Anti-FBXO3 antibody (ab224603)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FBXO3 (green) using ab224603 at 1/100 dilution in ICC/IF.
Secondary antibody: Alexa Fluor® 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab224603 尚未被引用在任何文献中。