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重组RabMAb

重组Anti-FBXO21抗体[EPR13163] (ab179818)

Submit a review Submit a question References (3)
Western blot - Anti-FBXO21 antibody [EPR13163] (ab179818)
  • Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] (ab179818)
  • Flow Cytometry (Intracellular) - Anti-FBXO21 antibody [EPR13163] (ab179818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (ab179818)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (ab179818)
  • Anti-FBXO21 antibody [EPR13163] (ab179818)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13163] to FBXO21
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-FBXO21抗体[EPR13163]
    参阅全部 FBXO21 一抗

  • 描述

    兔单克隆抗体[EPR13163] to FBXO21

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-P, WB, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Recombinant fragment within Human FBXO21. The exact sequence is proprietary.
    Database link: O94952

  • 阳性对照

    • MCF-7, HeLa, HepG2 and U87-MG cell lysates; Human brain and kidney tissues; Hela cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab179818于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Predicted molecular weight: 72 kDa.
IP
1/10 - 1/100.
说明
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Predicted molecular weight: 72 kDa.
IP
1/10 - 1/100.

靶标

图片

  • Western blot - Anti-FBXO21 antibody [EPR13163] (ab179818)
    Western blot - Anti-FBXO21 antibody [EPR13163] (ab179818)
    All lanes : Anti-FBXO21 antibody [EPR13163] (ab179818) at 1/10000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : U-87 MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 72 kDa

  • Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] (ab179818)
    Immunoprecipitation - Anti-FBXO21 antibody [EPR13163] (ab179818)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling FBXO21 with ab179818.

  • Flow Cytometry (Intracellular) - Anti-FBXO21 antibody [EPR13163] (ab179818)
    Flow Cytometry (Intracellular) - Anti-FBXO21 antibody [EPR13163] (ab179818)

    Intracellular flow cytometric analysis of permeabilized HeLa cells labeling FBXO21 with ab179818 at 1/10 dilution (red),compared toa rabbit IgG negative control (green). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (ab179818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (ab179818)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling FBXO21 with ab179818 at 1/50 dilution.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (ab179818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXO21 antibody [EPR13163] (ab179818)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling FBXO21 with ab179818 at 1/50 dilution.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-FBXO21 antibody [EPR13163] (ab179818)
    Anti-FBXO21 antibody [EPR13163] (ab179818)

文献 (3)

发表研究结果有使用 ab179818？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab179818 被引用在 3 文献中.

  • Lin Z  et al. JUNB-FBXO21-ERK axis promotes cartilage degeneration in osteoarthritis by inhibiting autophagy. Aging Cell 20:e13306 (2021). PubMed: 33450132
  • Yu Z  et al. Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response. Elife 5:N/A (2016). WB . PubMed: 27063938
  • Herr P  et al. A genome-wide IR-induced RAD51 foci RNAi screen identifies CDC73 involved in chromatin remodeling for DNA repair. Cell Discov 1:15034 (2015). Human . PubMed: 27462432

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