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AB17018

Anti-FBXL2 抗体

Anti-FBXL2 antibody

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(2 Publications)

Goat Polyclonal FBXL2 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FBXL2 aa 1-50.

查看别名

FBL2, FBL3, FBXL2, F-box/LRR-repeat protein 2, F-box and leucine-rich repeat protein 2, F-box protein FBL2/FBL3

2 Images
Western blot - Anti-FBXL2 antibody (AB17018)
  • WB

Supplier Data

Western blot - Anti-FBXL2 antibody (AB17018)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-FBXL2 antibody (ab17018) at 0.3 µg/mL

Lane 1:

Human brain tissue lysate at 35 µg

Lane 2:

Rat brain tissue lysate at 35 µg

Predicted band size: 47 kDa

false

Western blot - Anti-FBXL2 antibody (AB17018)
  • WB

Unknown

Western blot - Anti-FBXL2 antibody (AB17018)

ab17018 at 0.5μg/ml detecting FBXL2 from Human kidney lysate (35μg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence. ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence.

All lanes:

Western blot - Anti-FBXL2 antibody (ab17018)

Predicted band size: 47 kDa

false

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Rat, Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human FBXL2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UKC9

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5.00000-10.00000 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.30000-1.00000 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.30000-1.00000 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

FBXL2 also known as F-box and leucine-rich repeat protein 2 is involved in protein degradation mechanisms. It is part of a SCF (SKP1-Cullin-F-box) complex and functions as a ubiquitin ligase. FBXL2 targets proteins for ubiquitination tagging them for proteasomal degradation. Weighing approximately 49 kDa FBXL2 shows expression in various tissues including the liver heart and brain. Its role in maintaining protein homeostasis highlights its importance in cellular processes.
Biological function summary

The FBXL2 protein maintains cellular protein quality by regulating the turnover of specific substrates. It forms a complex with SKP1 and Cullin-2 in the ubiquitin-proteasome pathway. FBXL2 mediates the degradation of important regulatory proteins influencing the cell cycle and signal transduction. This activity impacts cellular responses to stress and environmental changes ensuring proper cellular adaptation.

Pathways

FBXL2 participates in the cell cycle and apoptosis regulation pathways. It intersects with the PI3K/Akt signaling pathway where it interacts with proteins like Cyclin D1 and Cdk inhibitors playing a part in cell proliferation and survival. These interactions demonstrate FBXL2's important role in controlling cell growth and division affecting how cells respond to intrinsic and extrinsic signals.

FBXL2 connects to cancer and neurodegeneration. In cancer FBXL2 may impact tumor progression due to its role in modulating oncogenic pathways interacting with proteins like mTOR which regulate cell growth. Additionally in neurodegenerative diseases FBXL2 affects protein aggregation processes which are relevant in conditions like Alzheimer's disease by targeting specific substrates for degradation. This connection to key disease mechanisms highlights FBXL2 as a significant focus for therapeutic research.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 22020328, PubMed : 22323446). Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin (PubMed : 22020328, PubMed : 22323446). This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin (PubMed : 22020328, PubMed : 22323446). Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0) (PubMed : 22020328, PubMed : 22323446). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy (PubMed : 23604317). PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (By similarity). The SCF(FBXL2) complex acts as a regulator of inflammation by mediating ubiquitination and degradation of TRAF proteins (TRAF1, TRAF2, TRAF3, TRAF4, TRAF5 and TRAF6) (By similarity). The SCF(FBXL2) complex acts as a negative regulator of the NLRP3 inflammasome by mediating ubiquitination and degradation of NLRP3 (PubMed : 26037928).
See full target information FBXL2
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 10:1706-1721 PubMed32690540

2020

Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chengheng Liao,Yang Zhang,Cheng Fan,Laura E Herring,Juan Liu,Jason W Locasale,Mamoru Takada,Jin Zhou,Giada Zurlo,Lianxin Hu,Jeremy M Simon,Travis S Ptacek,Victor G Andrianov,Einars Loza,Yan Peng,Huanghe Yang,Charles M Perou,Qing Zhang

Oncology letters 14:3255-3260 PubMed28927074

2017

miRNA-346 promotes proliferation, migration and invasion in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Yu,Xia Yang,Weidong Duan,Chonghui Li,Ying Luo,Shichun Lu
View all publications
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