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Anti-FBXL17抗体(ab111683)

Submit a review Q&A (1)References (1)
Western blot - Anti-FBXL17 antibody (ab111683)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL17 antibody (ab111683)

Key features and details

  • Rabbit polyclonal to FBXL17
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-FBXL17抗体

  • 描述

    兔多克隆抗体to FBXL17

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Recombinant fragment within Human FBXL17. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Recombinant fragment, corresponding to a region within Human FBXL17, centre region.

  • 阳性对照

    • MCF7 whole cell lysate; U373 xenograft tissue.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    ab111683 is purified by antigen-affinity chromatography.

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab111683于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

图片

  • Western blot - Anti-FBXL17 antibody (ab111683)
    Western blot - Anti-FBXL17 antibody (ab111683)
    Anti-FBXL17 antibody (ab111683) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 34 kDa



    10% SDS-PAGE.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL17 antibody (ab111683)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL17 antibody (ab111683)
    ab111683 at 1/500 dilution staining FBXL17 in paraffin-embedded U373 xenograft tissue by Immunohistochemistry.

文献 (1)

发表研究结果有使用 ab111683？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab111683 被引用在 1 文献中.

  • Lai Y  et al. Lipopolysaccharide modulates p300 and Sirt1 to promote PRMT1 stability via an SCFFbxl17-recognized acetyldegron. J Cell Sci 130:3578-3587 (2017). PubMed: 28883095

客户评价及客户问答

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Question

WB with Hela cells sees multiple bands but none at 72 kDa, a weak at around 35 kDa (isoforms) used also siRNA (4 different sequences) to reduce signal, but saw no reduction at all, sees however biological phenotype with siRNA (but not in WB) Ab: 1/1000 block: 5% milk secondary: OK, works well

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab68755. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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