重组Anti-FBP2抗体[EPR7436] (ab131253)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7436] to FBP2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-FBP2抗体[EPR7436]
参阅全部 FBP2 一抗 -
描述
兔单克隆抗体[EPR7436] to FBP2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human FBP2 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
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阳性对照
- Human skeletal muscle, Rat muscle and Mouse muscle lysates; Human muscle tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.90 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7436 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131253于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 37 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 37 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations and probably participates in glycogen synthesis from carbohydrate precursors, such as lactate. -
组织特异性
Expressed in skeletal muscle (at protein level). -
通路
Carbohydrate biosynthesis; gluconeogenesis. -
序列相似性
Belongs to the FBPase class 1 family. -
细胞定位
Cell junction. Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line. In neonatal cardiomyocytes, distributed throughout the cytosol, accumulating in the intercalated disks which occur at the Z line of cardiomyocytes and connect adjacent cells, and also located in the nucleus; dissociates from the Z line following an increase in cytosolic Ca(2+) concentration (By similarity). In muscle precursor cells, localizes predominantly to the nucleus and to a lesser extent to the cytoplasm at the proliferative phase, while mainly localizing to the cytoplasm at the differentiation phase (By similarity). Colocalizes with ALDOA and alpha-actinin on both sides of the Z line of skeletal muscle; dissociates rapidly from the Z line following an increase in cytosolic Ca(2+) concentration. - Information by UniProt
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数据库链接
- Entrez Gene: 8789 Human
- Entrez Gene: 14120 Mouse
- Entrez Gene: 114508 Rat
- Omim: 603027 Human
- SwissProt: O00757 Human
- SwissProt: P70695 Mouse
- SwissProt: Q9Z1N1 Rat
- Unigene: 61255 Human
see all -
别名
- 6-bisphosphatase isozyme 2 antibody
- 6-bisphosphate 1-phosphohydrolase 2 antibody
- D fructose 1 6 bisphosphate 1 phosphohydrolase 2 antibody
see all
图片
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All lanes : Anti-FBP2 antibody [EPR7436] (ab131253) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Rat muscle tissue lysate
Lane 3 : Mouse muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 37 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBP2 antibody [EPR7436] (ab131253)
Immunohistochemical analysis of paraffin embedded Human muscle tissue labelling FBP2 with ab131253 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab131253 被引用在 3 文献中.
- Huangyang P et al. Fructose-1,6-Bisphosphatase 2 Inhibits Sarcoma Progression by Restraining Mitochondrial Biogenesis. Cell Metab 31:174-188.e7 (2020). PubMed: 31761563
- Takahashi K et al. Effect of post-exercise lactate administration on glycogen repletion and signaling activation in different types of mouse skeletal muscle. Curr Res Physiol 3:34-43 (2020). PubMed: 34746818
- Wang C et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One 9:e101315 (2014). WB . PubMed: 24983750