Anti-FBH1抗体[2353C1a] (ab58881)
![Western blot - Anti-FBH1 antibody [2353C1a] (ab58881)](https://www.abcam.cn/ps/products/58/ab58881/Images/ab58881-72760-anti-fbh1-antibody-2353c1a-western-blot.gif "Western blot - Anti-FBH1 antibody [2353C1a] (ab58881)")
Key features and details
- Mouse monoclonal [2353C1a] to FBH1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-FBH1抗体[2353C1a] -
描述
小鼠单克隆抗体[2353C1a] to FBH1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human FBH1.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
克隆
单克隆 -
克隆编号
2353C1a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58881于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 118 kDa.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 118 kDa. |
靶标
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功能
DNA-dependent ATPase. Unwinds double-stranded DNA in a 3' to 5' direction.
Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. -
序列相似性
Belongs to the helicase family. UvrD subfamily.
Contains 1 F-box domain. -
细胞定位
Nucleus. - Information by UniProt
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别名
- F box DNA helicase 1 antibody
- F box only protein 18 antibody
- F box only protein helicase 18 antibody
see all
图片
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Western blot - Anti-FBH1 antibody [2353C1a] (ab58881)Anti-FBH1 antibody [2353C1a] (ab58881) + immunizing recombinant fragment
Predicted band size: 118 kDa
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
数据表及文件
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Datasheet download
文献 (4)
ab58881 被引用在 4 文献中.
- Liu G et al. Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases. Nat Commun 14:3024 (2023). PubMed: 37230987
- Kang JW et al. PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells. Signal Transduct Target Ther 6:129 (2021). PubMed: 33785736
- Mason JM et al. Non-enzymatic roles of human RAD51 at stalled replication forks. Nat Commun 10:4410 (2019). PubMed: 31562309
- Ronson GE et al. PARP1 and PARP2 stabilise replication forks at base excision repair intermediates through Fbh1-dependent Rad51 regulation. Nat Commun 9:746 (2018). PubMed: 29467415
