Anti-FATS抗体(ab122497)
Key features and details
- Rabbit polyclonal to FATS
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-FATS抗体 -
描述
兔多克隆抗体to FATS -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human FATS aa 169-262.
Sequence:AEDTLFQAPPALANGAHPGRHQRSFACTEFSRNSSVVRLKVPEAHTGLCE RRKYWVTHADDKETSFSPDTPLSGKSPLVFSSCVHLRVSQQCPD
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阳性对照
- Human kidney tissue; RT-4 and U-251 MG cell lysate; Human Liver and Tonsil tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122497于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/250 - 1/500.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/250 - 1/500. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 118611 Human
- SwissProt: Q96M02 Human
- Unigene: 587663 Human
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别名
- bA422P15.2 antibody
- C10orf90 antibody
- Chromosome 10 open reading frame 90 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FATS antibody (ab122497)
ab122497 at 1/425 staining FATS in Paraffin Embedded Human kidney tissue.
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Immunocytochemistry/ Immunofluorescence - Anti-FATS antibody (ab122497)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122497 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Western blot - Anti-FATS antibody (ab122497)All lanes : Anti-FATS antibody (ab122497) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122497 被引用在 1 文献中.
- Song F et al. A functional genetic variant in fragile-site gene FATS modulates the risk of breast cancer in triparous women. BMC Cancer 15:559 (2015). IHC . PubMed: 26223354